Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,4550GT40.0% 178.7 / 59.1 110intergenic (+853/+170)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base G (14/52);  new base T (44/0);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 9.44e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 110 total aligned reads displayed.

GTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTAT  >  NC_000913/3270310‑3270604
                                                                                                                                                 |                                                                                                                                                         
gTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGctctc                                                                                                                                                      <  3:117758/151‑1 (MQ=255)
 tACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTctct                                                                                                                                                       >  1:230159/1‑149 (MQ=255)
    gggCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGGTCTTTGtt                                                                                                                                                  >  3:616893/1‑151 (MQ=255)
    gggCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGCTTTCTCTCTGt                                                                                                                                                   >  3:779906/1‑150 (MQ=255)
      gCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGc                                                                                                                                                <  3:27251/151‑1 (MQ=255)
       ccGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCa                                                                                                                                               <  1:1390080/151‑1 (MQ=255)
          tACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGccc                                                                                                                                              >  4:423408/1‑147 (MQ=255)
           aCCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCACt                                                                                                                                            >  4:326247/1‑149 (MQ=255)
            ccTTATGATCCCCGATTTGGCCTTGCTCCGGGTGGAGTTTACCTTTCCACGGACTTTTACCAGCCGCGCGGTGCGCTCTTACCGCTCCCTTTCGCCCTTACCTGTTCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTgg                                                                                                                                          <  1:928043/150‑1 (MQ=255)
            ccTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGt                                                                                                                                         <  1:379058/151‑1 (MQ=255)
             cTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGGTTGCTCCCtttt                                                                                                                                                  >  1:816536/1‑142 (MQ=255)
               tATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTTCCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTg                                                                                                                                           <  3:1065794/146‑1 (MQ=255)
               tATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTCGCAc                                                                                                                                              >  4:387659/1‑144 (MQ=255)
               tATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCg                                                                                                                                     <  1:1299037/150‑1 (MQ=255)
                aTGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCACACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGt                                                                                                                                         >  3:1039052/1‑147 (MQ=255)
                    aCCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCACTGGTCgcg                                                                                                                                   >  3:720906/1‑145 (MQ=255)
                    aCCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGtt                                                                                                                              <  2:423412/151‑1 (MQ=255)
                    aCCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGtt                                                                                                                              <  2:413260/151‑1 (MQ=255)
                      cccTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGCTTTCTCTCTGTTGCACTTGTTGTGGGgtt                                                                                                                             >  3:1012529/1‑150 (MQ=255)
                       ccTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCCCTTGGTCGGGGGtt                                                                                                                              >  4:431595/1‑149 (MQ=255)
                       ccTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCCCTGGTCGTggggt                                                                                                                              >  1:1080205/1‑146 (MQ=255)
                         tATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCCCTTTTGCACTTGTCGTGGGGTTcccc                                                                                                                         >  1:673730/1‑151 (MQ=255)
                          aTTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTGTCTCTGTTGCACTCGTCGTGGGTTTtcccc                                                                                                                        >  1:978006/1‑151 (MQ=255)
                          aTTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTcccc                                                                                                                         <  3:819579/150‑1 (MQ=255)
                          aTTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTccc                                                                                                                          <  1:370448/149‑1 (MQ=255)
                            ttGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTcccccc                                                                                                                       <  1:546316/150‑1 (MQ=255)
                             tGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCACTTGTCGTGGGTTTtcccccc                                                                                                                      >  2:178033/1‑150 (MQ=255)
                             tGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTccccccc                                                                                                                      <  1:235946/150‑1 (MQ=255)
                              ggCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCCCTGGGCGTGGGGTTtcccccc                                                                                                                      >  3:700537/1‑149 (MQ=255)
                              ggCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAg                                                                                                                    <  3:1113989/151‑1 (MQ=255)
                                ccTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCACTGGTCGTGGGGTTcccccccc                                                                                                                     >  2:156509/1‑147 (MQ=255)
                                ccTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGc                                                                                                                  <  4:385424/151‑1 (MQ=255)
                                ccTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGc                                                                                                                  <  4:487556/151‑1 (MQ=255)
                                    gCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCACTGGTCGGGGGGTTCCCCCCCCCGCGtt                                                                                                               >  3:836250/1‑150 (MQ=255)
                                    gCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGtt                                                                                                               <  3:822541/150‑1 (MQ=255)
                                     cTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTTTTTCACTGGTCGTGGGGTTTccccccccgcggtac                                                                                                              >  1:1206375/1‑143 (MQ=255)
                                     cTCCGGGAGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGCTTTCTCTCTGTTGCACTGGTAGTATGGTTCCCCCCCACGc                                                                                                                  >  1:1047010/1‑146 (MQ=255)
                                      tCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGTTCTCTGTTTCCCCGGTCGGGGGGTTCCCCCCCCGGCGGTAcc                                                                                                            >  1:1178358/1‑151 (MQ=255)
                                       ccGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTAcc                                                                                                            <  3:887751/150‑1 (MQ=255)
                                        cGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCACTTGGCGGGGGGTTTCCCCCCCGGCGCTAcc                                                                                                            >  3:87730/1‑149 (MQ=255)
                                             ggggTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGTACCGGTCCGGGGGTTTCCCCCCCGGCCGtacccggccc                                                                                                       >  3:620330/4‑141 (MQ=255)
                                             ggAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCAc                                                                                                      <  2:187220/150‑1 (MQ=255)
                                               aGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCt                                                                                                   <  4:285467/151‑1 (MQ=255)
                                               aGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCt                                                                                                   <  3:526687/151‑1 (MQ=255)
                                                     ccGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCCCTCGTCGTGGGTTTTCCCCCCAAGCGGTACCCCGCACCCCGccc                                                                                               >  2:370602/1‑149 (MQ=255)
                                                      cGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTAt                                                                                            <  3:926001/151‑1 (MQ=255)
                                                       gTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTGCCCGGTCGGGGGGTTTCCCCCCCCGGGGTACCCTGCACCCCGccc                                                                                               >  1:554476/1‑147 (MQ=255)
                                                          ccACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGGCGTGGGGTTTCCCCCCCGGCGGTACCCGGCCCCCCGccc                                                                                               >  3:377795/1‑144 (MQ=255)
                                                              ggACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCACTGGTCGTGGGGTTCCCCCCCCGGCGTTTCCCGGCACCCCGCCCCATTGAGccc                                                                                     >  2:223407/1‑150 (MQ=255)
                                                                aCTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGGTTCCCCCCCCGGCGGTACCTGGCACCCCGCCCTATTGAGccc                                                                                     >  2:177495/1‑148 (MQ=255)
                                                                       ccAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCACTGGTCGTGGGGTTTCCCCCCCCGCGTTACCTGGCACCCCCCCCCATGGAGCCCCGGCttttc                                                                            >  1:62479/1‑148 (MQ=255)
                                                                       ccAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTg                                                                                                  <  2:208788/128‑1 (MQ=255)
                                                                       ccAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTtcct                                                                           <  3:1377343/151‑1 (MQ=255)
                                                                        cAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCTCCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTtcctc                                                                          <  4:245516/151‑1 (MQ=255)
                                                                        cAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTtcctc                                                                          <  4:44130/151‑1 (MQ=255)
                                                                           ccGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctcccc                                                                       <  1:609362/151‑1 (MQ=255)
                                                                           ccGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctcccc                                                                       <  3:958500/151‑1 (MQ=255)
                                                                              cgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctcccctcc                                                                    <  3:835662/151‑1 (MQ=255)
                                                                               gcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCg                                                                   <  1:719772/151‑1 (MQ=255)
                                                                                cgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCACCGGTCGTGGGGTTTCCCCCCCCGCGGTACCTGGCACCCCGCCCCCTTGGGCCCGGGCTTTcctccccccccc                                                                  >  1:320982/1‑149 (MQ=255)
                                                                                cgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGGCATCGTCGTGGGTTTCCCCCCCCGGCGTTTCCTGGCACCCCGCCCCCTGGAGCCCGGCCTTTcctccccccc                                                                    >  4:410394/1‑149 (MQ=255)
                                                                                  gTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGCTTTCTCTCTTTTTCCCTGGTCGTGGGGTTTCCCCCCCGGCGGTTCCTGGCACCCTGCCCCATGGAGGCCGGACTTTcccccccccccccc                                                                >  1:413493/1‑151 (MQ=255)
                                                                                    gcgcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCCCTTTTGCAAAAGAAGTGGGGTTTCCCCCCCCGCGGTTCCTGGCACCCCTCCCTCTCGAGCCCGGGCTTTTCTCCCCCCCGcc                                                                 >  4:206226/1‑148 (MQ=255)
                                                                                     cgcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGtc                                                             <  4:196117/151‑1 (MQ=255)
                                                                                      gcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCACTGGTCGTGGGGTTTCCCCCCCGGCGTTACCCTGCACCCTGCCCCCTGGGGCCCCGGCTTTCCTCcccccccccc                                                                >  3:813857/1‑147 (MQ=255)
                                                                                        tctTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTGTTTTCCTGGGCTTGGGGTTTCCCCCCCGGCGTTTCCCGGCCCCCTGCCCCCTTGGGGCCGGGCTTTCCTCCCCCCCCCCCgccccc                                                          >  1:672025/1‑146 (MQ=255)
                                                                                           tACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTTTTTTACTGGCCGTGGGGTTCCCCCCCCCCCGGTTCCCGGCACCCCTCCCCATTGAGGCCGGGCTTTCCCCCCCCCCCCCCGCCTccccc                                                       >  1:1308188/1‑151 (MQ=255)
                                                                                           tACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTcc                                                          <  4:270408/148‑1 (MQ=255)
                                                                                             ccGCCCCCTTTCCCCCTTCCCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCg                                                      <  3:398409/150‑1 (MQ=255)
                                                                                               gCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAag                                                   <  3:528206/151‑1 (MQ=255)
                                                                                                 aCCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCCCTGGTCGTGGGGTTCCCCCCCCGGCGGTACCTGGCCCCCCTCCCCCTGGGGCCCCGGCTTTTCTCCCCCCCCCCCCCCTccccc                                                       >  1:1327890/1‑145 (MQ=255)
                                                                                                 aCCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCACTGGGCGTGGGGTTTCCCCCCCCGCGGTACCTGGCACCCCGCCCCATGGGGCCCGGGCTTTTCTCCCCTCCCCCCGCCTcccccc                                                      >  3:539440/1‑145 (MQ=255)
                                                                                                      ttCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgac                                              <  3:1307749/149‑1 (MQ=255)
                                                                                                       tCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCACTGGGCGTGGGGTTCCCCCCCCGGCGTTACCTGGCACCCCGCCCTATGGAGCCCGGACTTTCCTCCCCCCCGCCCCTCTCCCCCCAAGAgg                                                >  4:379124/1‑146 (MQ=255)
                                                                                                        cACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgacgac                                           <  4:314089/150‑1 (MQ=255)
                                                                                                        cACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgacgac                                           <  1:543512/150‑1 (MQ=255)
                                                                                                          cccTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCACTTGGCGCGGGGTTCCCCCCCCGGCGGTTACTCGCCCCCCGCCCCCTGGGGCCCGGGCCTTTCCCGCCCCCCCCCCCCTccccccgacagg                                                >  1:143381/1‑136 (MQ=255)
                                                                                                           ccTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGGTGCACTGGTCGTGGGGTTTCCCCCCCGGCGTTTCCCTGCACCCCCCCCCATTGAGGCCCGGCTTTTCTCCCCCCCCCCCCTCTCCCCCCCAGAgg                                                >  3:79803/1‑142 (MQ=255)
                                                                                                             ttACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCACTGGTCGTGGGGTTTCCCCCCCGGGGGTACCTGGCACCCCGCCCTATGGGGCCCGGGGCTTTCTCCCCCCCCCCCCCCTCCCCCCCAGAgg                                                >  1:993465/1‑140 (MQ=255)
                                                                                                             ttACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTTTCTAGTCGTGGGGTTTCCCCCCAAGCGTTACCCGGTACCCCTCCCTATGGTGGCCGGGCGTTTCTCCCCTCCCCCCCTCTCCCCCCCAGATGAcgaccacga                                      >  3:660195/1‑150 (MQ=255)
                                                                                                              tACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCACTGGGCGGGGGGTTTCCCCCCCGGCGGTACCCGGCACCCCGCCCCATTGGGCCCCGGCTTTTCTCCCCCCCCCCCGCCTCCCCCCAAGAgg                                                >  4:543426/1‑139 (MQ=255)
                                                                                                               aCCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCACTTGTCGGGGGGTTTCCCCCCCGGCGTTTCCTGGCACCCCGCCCCATGGGGCCCGGGCTTTTCTCCCCCCCCCCCCTCcccccc                                                       >  1:1188696/1‑131 (MQ=255)
                                                                                                               aCCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGGCGTGGGGTTTCCCCCCCGGCGTTAACTGGCAACCCGCCCTATGGAGCCCCGGCTTTCCTCCCCTCCCCCCCTCTCCCCCCCAGAGGACCACGACCaa                                     >  3:332521/1‑149 (MQ=255)
                                                                                                                ccTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAgcg                                  <  4:341576/151‑1 (MQ=255)
                                                                                                                  tGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCACTGGTCGTGGGGTTTCCCCCCCGGCGGTACCTGGCACCCCCCCCCATGGAGCCCCGGCCTTCCTCCCCCCCCCCCGGCTCCCCCCCAGAGGACGACGACGAAacgg                                 >  3:84911/1‑150 (MQ=255)
                                                                                                                       ccGCTTGCGCGGGCCATCGGCGGATTGCTTTCTCTTTTACTTGTCCGGGGTTTCCCCCCCCGGCGTTAGCCCGCCCCCCGCCCTATGGGGCCCCGACCTTTCTCCCCCCCCCCCCTCCCCCCCCCAGAGgacggcgcgggagcg                                  >  3:339648/1‑136 (MQ=255)
                                                                                                                        cGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGt                          <  4:16477/151‑1 (MQ=255)
                                                                                                                             gcgcGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTgg                      <  3:55792/150‑1 (MQ=255)
                                                                                                                             gcgcGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGt                     <  1:700438/151‑1 (MQ=255)
                                                                                                                               gcgGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCa                   <  3:191375/151‑1 (MQ=255)
                                                                                                                                  ggCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCt                <  2:431601/151‑1 (MQ=255)
                                                                                                                                   gCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCtt               <  3:1206510/151‑1 (MQ=255)
                                                                                                                                   gCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCtt               <  3:656229/151‑1 (MQ=255)
                                                                                                                                        cggcggTTTGCTCTCTGTTGCACTGGTCGCGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgc           >  2:152451/1‑150 (MQ=255)
                                                                                                                                           cggTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc       >  3:394464/1‑151 (MQ=255)
                                                                                                                                             gTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAg     <  1:1038922/151‑1 (MQ=255)
                                                                                                                                               ttGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGt    >  1:322534/1‑150 (MQ=255)
                                                                                                                                               ttGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGt    >  1:298126/1‑150 (MQ=255)
                                                                                                                                               ttGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGCCAGCTTCGGCGCGCa      >  1:1043869/1‑148 (MQ=255)
                                                                                                                                                tGCTCTCTGTTGCACTGGTCGTGGGTTTTCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGtat  >  2:496233/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                         
GTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTAT  >  NC_000913/3270310‑3270604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: