Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,350,8530AC41.5% 176.3 / 13.3 95S725A (TCA→GCA) arcBaerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base A (47/8);  new base C (0/39);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 1.50e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTT  >  NC_000913/3350704‑3351002
                                                                                                                                                       |                                                                                                                                                     
ttttGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAc                                                                                                                                                      >  3:19394/1‑151 (MQ=255)
   tGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                                                                                                                                                   >  3:1320689/1‑151 (MQ=255)
   tGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                                                                                                                                                   >  1:459535/1‑151 (MQ=255)
      cacccacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccgcc                                                                                                                                                <  1:1257553/151‑1 (MQ=255)
      cacccacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccgcc                                                                                                                                                <  1:73937/151‑1 (MQ=255)
         ccacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCcgccgc                                                                                                                                              >  2:236200/1‑150 (MQ=255)
           acGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCAcc                                                                                                                                           >  1:1211416/1‑151 (MQ=255)
           acGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCAcc                                                                                                                                           >  3:120402/1‑151 (MQ=255)
                ttCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTa                                                                                                                                       >  1:330016/1‑150 (MQ=255)
                      aCTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTAt                                                                                                                                >  1:1322933/1‑151 (MQ=255)
                      aCTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTAt                                                                                                                                >  3:331776/1‑151 (MQ=255)
                        ttCTCCGTCGTGCCCCCCCCCTTCTTTCTTCTCTTAAACCCTTCCCCCGATGTTTTTTCCCC‑AGGCCGGAAGGTCAGGAAACTTAATTTG‑CTCCCCTTGTTTTTGAAGTGGGGGTACCCCCCCGGCCCCCGCCGCACCTTTAATTTTAt                                                                                                                                <  4:77337/149‑1 (MQ=255)
                              gtcgtGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGtccttcc                                                                                                                        >  3:616430/1‑151 (MQ=255)
                               tcgtGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGtccttcct                                                                                                                       >  3:1075356/1‑151 (MQ=255)
                               tcgtGACCCCCCTCTTCTTTCATCTCTCCAACCCATCCACCGCTGTTATCTCCCC‑AGGCCGGAAGGTCAGGGAACTGATTTTT‑CTGACCCAGCTGTCGGAGTTGGCGAAACCCCTCGGTCCCCGCCGCACCTTTAATTTTATGtccttcc                                                                                                                        <  3:1317533/150‑1 (MQ=255)
                                  tGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTca                                                                                                                     >  1:797392/1‑150 (MQ=255)
                                  tGACCCCCCCCTTCTTCCACCTCTAAAACCCATCCCCCGACTTTTTCTCCCC‑GGCCCGGAGGTGAAGGGCACTAATTTTG‑CTCACCCAGCTTTTGCAGAGGGCGTACCCCCCCGGCCCCCGCCGCACCTTTAATTTTATGtccttc                                                                                                                         <  1:93368/146‑1 (MQ=255)
                                     cccccctctTTTTTCATCTCTTCAACCCATCCCCCGCCTTTATCTCCCC‑GGGCGGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGTTGTTGCAGAGGGCGTACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTcaaca                                                                                                                  <  3:510288/145‑1 (MQ=255)
                                     cccccctctTCTTCCATCTCTTAAACCCATCCCCCGACTTTATCTCCCC‑AGGCGGGAAGGTCGGGAGATTAATTTTC‑CTGCCCTATTTTTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTcaac                                                                                                                   <  4:301104/144‑1 (MQ=255)
                                      cccccttttcttcCATCTCTTCAACCCATCCCCCGACGTTATCTCCCA‑GGGCGGGAGGGTCAGGAGACTGAATTTG‑CTGACCCAGTTGTTGCAGAGGGCGAACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAAt                                                                                                                <  3:522050/144‑1 (MQ=255)
                                       cccctctTTTTTCATCTCTTCAATCCATCCCCCGCCGTTATCTTCCC‑AGGCCGGAGGGTCAGGAGACTAATTTTG‑CTGCCCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATg                                                                                                               <  3:187218/148‑1 (MQ=255)
                                       ccACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATg                                                                                                               >  1:151567/1‑151 (MQ=255)
                                            cttcttTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCttt                                                                                                          >  4:358009/1‑151 (MQ=255)
                                              tcttTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCttttt                                                                                                        >  4:111663/1‑151 (MQ=255)
                                                tttCATCTCTTCAATCCATTCCCCGACGTTATCTTCCC‑AGGCCGGAGGGTCAGGAGACTGAATTTG‑CTCACCCAGCTGTTGCAGAGGGCTTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtt                                                                                                           <  2:547233/146‑1 (MQ=255)
                                                tttCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtttttt                                                                                                       >  4:104228/1‑150 (MQ=255)
                                                tttCATCTCTTCAACCCATTCCCCGACTTTATCTCCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGCCCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCttt                                                                                                          <  3:568499/147‑1 (MQ=255)
                                                tttCATCTCTACATGCCTTCCCCCGACTTTATCTTCCC‑GGCCCGGAGGGTTGGGAGGCTGATTGGG‑CTGACCCAGTTGTTGCAGAGGGGGTACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCttttt                                                                                                        <  1:499069/149‑1 (MQ=255)
                                                 ttctcctgttCAACCCATCCCCCATCTTTATCTTCCA‑AGCCAGGGAGGTCGGGAAACAGATTTTT‑CTGCCCCAGTTGTTGCAGATGGCGAAACCCCCTTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTa                                                                                                      <  3:1378747/142‑1 (MQ=255)
                                                   cATCTCTTCAACCCATCCACCGACTTTATCTCCCC‑AGGCCGGAAGGCCAGGAGACTAATTTGG‑CTACCCCATTTGTTGCAGTGGGCGTACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCttttt                                                                                                        <  1:904760/146‑1 (MQ=255)
                                                      ctctCCAATCCATCCCCCGCCGTTATCTCCCC‑AGGCGGGAAGGTCAGGAGATTGAATTTG‑CTGACCCGGCTGTTGCAGATGGCGTACCCCCACTAACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATc                                                                                                    <  2:540767/147‑1 (MQ=255)
                                                      ctctCCAACCCATCCCCCGACTTTATCTCCCC‑AGGCGGGAGGGCCAGGAGACTGATTTGG‑CTCCCCCATCTTTTGAAGGGGGCGTACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATcc                                                                                                   <  1:166487/148‑1 (MQ=255)
                                                      ctcctcAACCCATCCGCCCACTTTTTCTCCCC‑AGGCCGGAAGGTCAGAAGAATGATTTTG‑CTAACCCTGCTGTTCCAAATGGCAAACCCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtt                                                                                                           <  3:1280970/140‑1 (MQ=255)
                                                        ctTCAACCCATCCCCCGACGTTATCTCCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTg                                                                                                 <  3:1202578/148‑1 (MQ=255)
                                                         ttCAATCCATCCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTCACCCAGTTGTTGCAGATGGCGTACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTgc                                                                                                <  4:540816/148‑1 (MQ=255)
                                                          tCAATCCATTCACCGACGTTATGTTCCA‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTgcg                                                                                               >  4:245116/1‑148 (MQ=255)
                                                           cAACCCATCCCCCGACGTTTTCTCCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGCCCCAGCTGTTGCAGATGGCGTAACCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCg                                                                                            <  2:93061/150‑1 (MQ=255)
                                                            aacccATCCACCGACGTTATCTCCCC‑AGGCGGGAAGGCCAGGAGACTAATTTTG‑CTGCCCCGGCTGTTGCAGAGGGCTTACCCCCACTGCCCCCGCCGCACCTTAAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCg                                                                                            <  3:505359/146‑1 (MQ=255)
                                                            aaTCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGt                                                                                           >  1:168163/1‑150 (MQ=255)
                                                              ccaatcccccGACGTTATCTTCCC‑AGGCCGGAGGGTCAGGAGACTAAATTTG‑CTGCCCCAGTTGTTGCAGAGGGCGTACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCa                                                                                         <  2:515250/142‑1 (MQ=255)
                                                               ccATTCCCCGACTTTATCTCCCC‑AGGCCGGAAGGTCAGGAGACTGATTTTG‑CTGACCCAGTTGTTGCAGAGGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCa                                                                                         <  3:330052/149‑1 (MQ=255)
                                                                 atcccccGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAg                                                                                        <  3:452526/143‑1 (MQ=255)
                                                                  ttCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTc                                                                                    >  1:849713/1‑151 (MQ=255)
                                                                    ccccgcctttATCTCCCC‑GGGCCGGAAGGTCAGGAGACTGATTTTG‑CTGCCCCAGTTGTTGAAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCg                                                                                   <  1:778167/142‑1 (MQ=255)
                                                                    ccccGACTTTATCTCCCC‑GGGCCGGAAGGTCGGGAGACTGAATTTG‑CTGCCCCATTTTTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCg                                                                                            <  1:570402/139‑1 (MQ=255)
                                                                       cGACTTTTTCTTCCC‑AGGCCGGGAGGGCAGGGGCTTGATTTTG‑CTGCCCCAGCTGTTGCAGAGGGCGTACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTc                                                                               <  1:159855/151‑1 (MQ=255)
                                                                       cGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTc                                                                               >  4:493184/1‑151 (MQ=255)
                                                                          cGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTcc                                                                              >  1:1344145/1‑149 (MQ=255)
                                                                           gTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGc                                                                           >  4:244342/1‑151 (MQ=255)
                                                                            ttATCTCCCC‑AGGCCGGAGGGTCGGGAGACTGATTTTG‑CTGCCCCAGTTTTTGCAGATGCCGAACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAg                                                                            <  4:311120/149‑1 (MQ=255)
                                                                             tATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCAc                                                                         >  1:1352844/1‑151 (MQ=255)
                                                                             tATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCAc                                                                         >  1:622524/1‑151 (MQ=255)
                                                                               tCTTCCC‑AGGCCGGAAGGTCGGGAGACTAAATTTG‑CTCACCCATCTTTTGCAGATGGCGTACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCa                                                                          <  1:705765/148‑1 (MQ=255)
                                                                               acTTCCCGAGGGCAGAGGGTCAGGAGACTTATTGTG‑CTGCCCGAGCTTTTGCAGACGGCTTACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAACGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCAc                                                                         <  1:477399/149‑1 (MQ=255)
                                                                                cTTCCC‑AGGCCGGAAGGTCAGGAGACTAAATTTG‑CTCCCCCATTTTTTGCAGGTGGCTTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCAc                                                                         <  1:1027873/148‑1 (MQ=255)
                                                                                 tcccc‑AGGCCGAAAGGTCAGGAGACT‑AATTTGCCTGCCCCAGTTGTTGCAGAGGGCTTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCa                                                                          <  1:835531/144‑1 (MQ=255)
                                                                                      cacgCCGGAAGGGCGGGAGATTGATTCTG‑CTGACCCACCTGTTGCAGATGCCATCCCCCCCCTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGa                                                                    <  1:788033/145‑1 (MQ=255)
                                                                                       aGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACAt                                                                 >  4:208486/1‑150 (MQ=255)
                                                                                       aGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACAt                                                                 >  4:234492/1‑150 (MQ=255)
                                                                                        ggCCGGAGGGTCAGGAGACTGAATTTC‑CTGCCCCAGTTGTTGAAGATGGCGAACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATa                                                                <  1:389558/150‑1 (MQ=255)
                                                                                        ggCCGGAAGGCCGGGAGACTAAATTTG‑CTCACCCAGCTTTTGCAGAGGGCGAACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACAt                                                                 <  1:704557/149‑1 (MQ=255)
                                                                                           cGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGccc                                                            >  2:46979/1‑151 (MQ=255)
                                                                                           cGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGccc                                                            >  3:349060/1‑151 (MQ=255)
                                                                                               aGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGc                                                         >  3:1262274/1‑150 (MQ=255)
                                                                                                ggccAGGAGACTGAATTTG‑CTGACCCAGCTGTGGCAGTGGGCGTACCCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGcat                                                       <  1:1011415/148‑1 (MQ=255)
                                                                                                  tCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGcatc                                                      >  1:1354504/1‑150 (MQ=255)
                                                                                                    aGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCatcatc                                                   >  3:1141993/1‑151 (MQ=255)
                                                                                                     ggactcgcattttC‑CTGCCCCAGCTGTTGCAGATGCCTAACCCCCCCTTCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGcatcat                                                    <  1:677700/139‑1 (MQ=255)
                                                                                                     ggAGCCTAATTTTG‑CGGCCCCATTTGTTGCAGAGGGCGTACCCCCCCCAACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtct                                                  <  4:380437/151‑1 (MQ=255)
                                                                                                     ggAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtct                                                  >  3:1363829/1‑151 (MQ=255)
                                                                                                      gagaCTGATTTTC‑CTGCCCCATCTTTTGCAGATGGCTTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtctt                                                 <  4:401302/151‑1 (MQ=255)
                                                                                                       agaCTGATTTTC‑CGCCCCTAGCTGTTGCAGTTGGCTTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtcttc                                                <  1:858768/151‑1 (MQ=255)
                                                                                                          cTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTtctc                                              >  4:421863/1‑150 (MQ=255)
                                                                                                            gAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCaa                                            >  1:317902/1‑150 (MQ=255)
                                                                                                             aaTTTG‑CTGACCCAGCTGTTACAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTGTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCAAGAACGCTGACATAGCACGGCATCATCTTCTCAAac                                          >  4:13340/1‑151 (MQ=255)
                                                                                                               tttG‑CTGCCCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAaca                                         <  3:1220010/150‑1 (MQ=255)
                                                                                                               attG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAaca                                         >  1:928896/2‑150 (MQ=255)
                                                                                                                     tGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCt                                    >  3:1413929/1‑150 (MQ=255)
                                                                                                                      gACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTa                                   >  1:1387840/1‑150 (MQ=255)
                                                                                                                       aCCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTaa                                  <  3:667367/150‑1 (MQ=255)
                                                                                                                        cccAGTTTTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCt                                    <  3:832629/147‑1 (MQ=255)
                                                                                                                        cccAGCTGTTGCAGAGGGCGTAACCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCt                                    <  3:624368/147‑1 (MQ=255)
                                                                                                                         ccAGTTTTTGAAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAAccc                               <  2:100346/151‑1 (MQ=255)
                                                                                                                         ccAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAAccc                               >  3:529021/1‑151 (MQ=255)
                                                                                                                            gCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTc                            >  1:564977/1‑151 (MQ=255)
                                                                                                                                 tGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTg                        >  1:1314598/1‑150 (MQ=255)
                                                                                                                                   cAGATGGCGTAACCCCACTAACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGAt                      <  2:432433/150‑1 (MQ=255)
                                                                                                                                          cGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGAGATCAGCTTCg              >  4:380451/1‑151 (MQ=255)
                                                                                                                                            tAACCCCACTCACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACCATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATCGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCgg             >  4:506055/1‑150 (MQ=255)
                                                                                                                                             acccccccTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGt            <  2:437047/148‑1 (MQ=255)
                                                                                                                                               ccccccTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCgg             <  1:616345/147‑1 (MQ=255)
                                                                                                                                                cccACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCt         >  3:149567/1‑150 (MQ=255)
                                                                                                                                                 ccACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTAc       >  4:442923/1‑151 (MQ=255)
                                                                                                                                                      gACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACa      >  4:29870/1‑147 (MQ=255)
                                                                                                                                                      gACCCCACCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGtt  >  1:769163/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
TTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCC‑AGGCCGGAAGGTCAGGAGACTGAATTTG‑CTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTT  >  NC_000913/3350704‑3351002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑