Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,640,9810CT39.4% 123.7 / 50.5 94F40F (TTC→TTT) dtpBdipeptide and tripeptide permease B
Reads supporting (aligned to +/- strand):  ref base C (8/49);  new base T (37/0);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 1.11e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTT  >  NC_000913/3640832‑3641128
                                                                                                                                                     |                                                                                                                                                      
tttCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttc                                                                                                                                                        <  4:447318/150‑1 (MQ=255)
  tCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttctt                                                                                                                                                      <  3:1338289/150‑1 (MQ=255)
   cAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttcttc                                                                                                                                                     <  2:452030/150‑1 (MQ=255)
    aGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTttttttt                                                                                                                                                     >  2:287492/1‑148 (MQ=255)
      gATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTa                                                                                                                                                 <  4:369780/151‑1 (MQ=255)
       aTGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTaa                                                                                                                                                <  3:23207/151‑1 (MQ=255)
       aTGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTaa                                                                                                                                                <  1:1417905/151‑1 (MQ=255)
        tGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGCGCAGGGCGTACTGGCGGTtttttttttt                                                                                                                                                  >  1:949248/1‑144 (MQ=255)
         ggTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTaa                                                                                                                                                >  2:286229/1‑149 (MQ=255)
         ggTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAAc                                                                                                                                              <  3:1329067/151‑1 (MQ=255)
             ttGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTtaaaa                                                                                                                                             >  1:1041524/1‑146 (MQ=255)
               ggAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTtttttttttttaaaaactt                                                                                                                                         >  3:779094/1‑137 (MQ=255)
               ggAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑tt                                                                                                                                        <  1:833584/150‑1 (MQ=255)
                gAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTgg                                                                                                                                      <  3:270488/151‑1 (MQ=255)
                gAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTgg                                                                                                                                      <  2:198709/151‑1 (MQ=255)
                 aaGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTgg                                                                                                                                      <  2:239434/150‑1 (MQ=255)
                   ggTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTTAACAAC‑TTgtctt                                                                                                                                   >  1:751890/1‑147 (MQ=255)
                      taataaATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATtct                                                                                                                                 <  3:910438/150‑1 (MQ=255)
                             tatGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTTTAACAGC‑TTGGGtt                                                                                                                                   >  3:855235/1‑141 (MQ=255)
                              atGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑Gc                                                                                                                       <  3:1201917/151‑1 (MQ=255)
                                gAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAGC‑TTGGGTTTTCCCCaga                                                                                                                          >  3:681729/1‑147 (MQ=255)
                                gAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCa                                                                                                                      <  3:7683/150‑1 (MQ=255)
                                gAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCa                                                                                                                      <  1:1068988/150‑1 (MQ=255)
                                gAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑g                                                                                                                    <  1:403856/151‑1 (MQ=255)
                                  aTACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGc                                                                                                                  <  3:146995/151‑1 (MQ=255)
                                  aTACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGc                                                                                                                  <  3:1086027/151‑1 (MQ=255)
                                   tACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTAAAAAAC‑TTTGGTTTTCCCCAGA‑GAA‑g                                                                                                                    >  3:1313024/1‑148 (MQ=255)
                                      aacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCtttt                                                                                                              <  1:279600/151‑1 (MQ=255)
                                      aacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCtttt                                                                                                              <  2:139279/151‑1 (MQ=255)
                                      aacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTTAAAAGC‑TTGGGTTTTCCCCAGA‑GCA‑GGGtttt                                                                                                              >  1:29403/1‑151 (MQ=255)
                                       acaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAGC‑TTGGGTTCTCGCCAGA‑GCA‑GGCtttt                                                                                                              >  3:1022293/1‑150 (MQ=255)
                                       acaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTTTAAAAAC‑TTGGATTTTCGCAAAA‑GCA‑GGCtttt                                                                                                              >  1:529749/1‑150 (MQ=255)
                                       acaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTg                                                                                                             <  4:273875/151‑1 (MQ=255)
                                         aacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTc                                                                                                           <  4:483073/151‑1 (MQ=255)
                                            acCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCTTTAAAAAAC‑TTGGATTCTCGCAAGA‑GGA‑GGCtttttttac                                                                                                         >  2:241354/1‑145 (MQ=255)
                                             cccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAAC‑TTGGATTCTCCCCAGA‑GAA‑GGCTTTTTTTACtt                                                                                                       >  3:152376/1‑151 (MQ=255)
                                             cccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTTTAACAAC‑TTGGGTTTTTGCCAGA‑GGA‑GGCTTTTGTCACtt                                                                                                       >  3:94151/1‑151 (MQ=255)
                                              ccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTTTTAAAAC‑TTGGGTTTTCGCAAAA‑GCA‑GGGtttttttccctt                                                                                                      >  1:432898/1‑143 (MQ=255)
                                              ccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTTGTTAAAAAGC‑TTTGGTTCTCGCAAGA‑GCA‑GGCcttttttgcctt                                                                                                      >  3:998247/1‑143 (MQ=255)
                                              ccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTTGTTAAAAAGC‑CTTGGTTCTCCCAAAA‑AGA‑AGCTTTTTTTACttt                                                                                                      >  3:866506/1‑151 (MQ=255)
                                               cATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAACAGC‑CTGGGTTTTCGCCAGA‑GGA‑GGGTTTTTTTACtttt                                                                                                     >  2:39478/1‑151 (MQ=255)
                                               cATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACtttt                                                                                                     <  3:22631/151‑1 (MQ=255)
                                                aTGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTTTTTAAAAGC‑TTTGGTTTTCGCCAGA‑GCA‑GGGGTTTTTTACttttt                                                                                                    >  1:539905/1‑150 (MQ=255)
                                                 tGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAAG‑TTGGGTTCTTGCAAAA‑GGA‑GGCGTTTGGTACtttttg                                                                                                   >  3:735301/1‑149 (MQ=255)
                                                  ggggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAGC‑TTGGGTTCTCGCAAAA‑GCA‑GGGTTTTTTTACcttttg                                                                                                   >  3:963215/1‑148 (MQ=255)
                                                  ggggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGt                                                                                                  <  1:568421/151‑1 (MQ=255)
                                                   gggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAACAGC‑TTGGATTTTCGCCAGA‑GCC‑GGGTTTTTTCCCTTTTTGt                                                                                                  >  2:66841/1‑150 (MQ=255)
                                                   gggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTg                                                                                                 <  1:1089774/151‑1 (MQ=255)
                                                   gggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTg                                                                                                 <  3:658732/151‑1 (MQ=255)
                                                    ggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTTGTTAAAAAAA‑TTTGGTTTTTGCCAGA‑GCA‑ggggtttttctccttt                                                                                                     >  3:156950/1‑132 (MQ=255)
                                                         ctgcAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTTTTTAAAAAC‑TTTGGTTTTTGCAAGA‑GCA‑GGCCTTTTTTCCTTTTTGTGGttttt                                                                                           >  1:328623/1‑150 (MQ=255)
                                                           gcagcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTtgct                                                                                         <  3:1201331/151‑1 (MQ=255)
                                                                    tCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAGC‑CTTGGTTTTCCCCAAA‑GCC‑GGGTTTTTTTTCCTTTTGTGCTTTTTCTgcgccg                                                                                   >  1:222892/1‑146 (MQ=255)
                                                                     cGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTc                                                                                <  4:229047/150‑1 (MQ=255)
                                                                      gCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTTAACAGC‑TTTGGTTCTCGCAAGA‑GAC‑AGCTTTTTTTACTTTTTGTGGTTTTGTTTCGCTgg                                                                                  >  3:896444/1‑147 (MQ=255)
                                                                       cccATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAACC‑CTGGGGTTTCGCACGA‑GCA‑GGCTTTTTTTACTTTTTTTGTTTTTTCTGCGCTTGTCTAt                                                                             >  1:558896/1‑151 (MQ=255)
                                                                        ccATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATg                                                                            <  1:1252849/151‑1 (MQ=255)
                                                                          aTTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAACAAC‑CTTGGTTCTCGCAAAA‑GCA‑GGGTTTTTTCTCTTTTTGTGCTTTTTCTTCGCTGGTCTAt                                                                             >  1:283713/1‑148 (MQ=255)
                                                                            ttttCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTTGTTTAAAAAC‑TTTGGGTCCCGCACGAGGCA‑GG‑TTTTTTCTCTTTTGGGGGTTTTTTTGTGCTGGtgcat                                                                             >  4:423633/1‑142 (MQ=255)
                                                                             tttCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAACAGC‑TTTGGGTTTCGCCAGA‑GCA‑GGGGTTTTTTTCTTTTTGTGGTTTTTTTGTGCTGGTCTAttggc                                                                         >  1:174823/1‑145 (MQ=255)
                                                                             tttCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCt                                                                        <  2:114556/150‑1 (MQ=255)
                                                                              ttCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTc                                                                       <  2:258654/150‑1 (MQ=255)
                                                                                cATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCCTTAAACAGCTTTGGTTTTTCCAAAAA‑GAA‑GGATTTT‑TTACTTTTGGGGCTTTTTTTCACCTGGTGTATGGcttactt                                                                    >  3:1081971/1‑145 (MQ=255)
                                                                                   gATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTTAAAAAGC‑TTGGGTTTTCGCAAGA‑GGA‑GGCTTTTTTCACTTTTTGTGGTTTTTCTGCGCTGGGCTTTTGGCTCCttt                                                                   >  2:234352/1‑149 (MQ=255)
                                                                                   gATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTc                                                                  <  4:521290/150‑1 (MQ=255)
                                                                                   gATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGATTTTTTTCTTTAACAAA‑TTTGATTTTCGCAAGA‑GAA‑GGCTTTTTTCCATTTTTGTGCTTTTTTTCCGCTGGTCTAttggccccttt                                                                   >  1:1385779/1‑139 (MQ=255)
                                                                                    aTCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTTAAAAAGC‑TTTGGTTTTCGCAAGA‑GGA‑GGCTTTTTTCACTTTTTGTGGTTTTTCTGCGCTGGTCTATGGCCTCAttttc                                                                 >  2:457221/1‑148 (MQ=255)
                                                                                      cTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAACAGA‑TTTGGATCTCTGAAGA‑GGA‑GGCTTTTTTGACTTTTTTTGCGTTTTTTGCGCTGGGCTATTGGCTCCTTTCCAtt                                                              >  1:76092/1‑151 (MQ=255)
                                                                                      cTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTAAAAAGC‑CTGGGTTTTTGCAAAA‑GCC‑GGGTTTTTTTACTTTTTGTGGTTTTTCTTCGCTGGTCTATTGCCTCAttttcctt                                                              >  4:236578/1‑146 (MQ=255)
                                                                                        tttttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTcc                                                                 <  2:99326/146‑1 (MQ=255)
                                                                                        tttttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCAtt                                                              <  3:701754/149‑1 (MQ=255)
                                                                                         ttttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTgg                                                            <  3:367503/150‑1 (MQ=255)
                                                                                          tttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTggc                                                           <  1:1104753/150‑1 (MQ=255)
                                                                                            tGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTggcgg                                                         <  3:949374/150‑1 (MQ=255)
                                                                                             gTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCt                                                       <  3:326141/151‑1 (MQ=255)
                                                                                               cGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTAt                                                     <  3:1390833/151‑1 (MQ=255)
                                                                                                gAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATg                                                    <  1:594143/151‑1 (MQ=255)
                                                                                                   ttATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTTTAACAAC‑TTGGGTTTTCGCCAGA‑GCAGGGCTTTTTTC‑CTTTTTGTGCTTTTTCTTCGCTGGGCTTTGGCCTCATTTTCATTtgcgggtgt                                                     >  1:417205/1‑143 (MQ=255)
                                                                                                         gAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTAAACAGC‑CTGGGTTTTCGCAAAA‑ACA‑GGCTTTTTTCACTTTTTGGGGTTTTTCTGCGCTGGTCTTTTGGCTCATTTTCATTTGCGGCTTTGTTGGCGgcc                                           >  2:381876/1‑151 (MQ=255)
                                                                                                         gAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGacc                                           <  3:1372577/151‑1 (MQ=255)
                                                                                                            cGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTTAAAAAC‑TTTGGTTTTCGCAAAA‑GGA‑GGGTTTTTTTACTTTTTGGGGTTTTTTTGCGCTGGGCTATGGCCTCCTTTTCCTTTGGGGGTATGTTGGCGgccccc                                        >  3:1181989/1‑151 (MQ=255)
                                                                                                                 cGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACctgctg                                   <  3:1258549/151‑1 (MQ=255)
                                                                                                                     tactacGGCGTGCAGGGCGTACTGGCGGTTTTTTTCGTTTAACAGC‑TTTGGTTTTCGCAAGA‑GCA‑GGGGTTTTTTACTTTTTGTGCTTTTTTTGCGCTGGGCTATGGCCCCCTTTTCCTTTGCGGGTTTTTTGGGGGCCACCCGCTggggg                               >  3:719775/1‑150 (MQ=255)
                                                                                                                         acGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCa                            <  3:526030/150‑1 (MQ=255)
                                                                                                                            gCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTAAAAAGC‑TTGGATTCTCGCCAGA‑GCA‑GGCTTTTGTTACTTTTTGTGCTTTTTCTGCGCTGGGCTATGGCCTCATTTTCATTGGCGGCTCTGTCGGCGGCCACCCCCTGGGGGGCaaa                          >  3:1155526/1‑149 (MQ=255)
                                                                                                                            gCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACg                        <  1:416767/151‑1 (MQ=255)
                                                                                                                             cGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGc                       <  2:485121/151‑1 (MQ=255)
                                                                                                                              gTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCa                      <  2:407817/151‑1 (MQ=255)
                                                                                                                                  aGGGCGTACTGGCGGTTTTTTTTGTTTAACAGC‑TTGGGTTTTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTTGTGCTTTTGCTGCGCTGGGCTCTGGCCTCATTTCCCTTGGCGGCTATGTTGGCGACCCCCCTCTGGGGGCCCAACCCaac                    >  1:441572/1‑147 (MQ=255)
                                                                                                                                    ggCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTg                <  4:388101/151‑1 (MQ=255)
                                                                                                                                         aCTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCtt           <  1:694641/151‑1 (MQ=255)
                                                                                                                                          cTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGGCCAAACGCACCATTGTTCtt           >  1:825199/1‑150 (MQ=255)
                                                                                                                                          cTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCtt           >  1:409116/1‑150 (MQ=255)
                                                                                                                                           tGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTgg         >  2:288915/1‑151 (MQ=255)
                                                                                                                                           tGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCCTTTTCGGCGACCACCTTCTGGGGGACAAAAGCCCCAtt                 >  1:749566/1‑143 (MQ=255)
                                                                                                                                            ggCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTg          <  1:151961/149‑1 (MQ=255)
                                                                                                                                            ggCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGa        <  3:515461/151‑1 (MQ=255)
                                                                                                                                                gTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCAc    <  1:693230/151‑1 (MQ=255)
                                                                                                                                                   ttcttcGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGTCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGTGCAc    >  1:1307891/1‑148 (MQ=255)
                                                                                                                                                   ttcttcGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACtt  >  2:214091/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTTCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGC‑TTGGATTCTCGCAAGA‑GCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTT  >  NC_000913/3640832‑3641128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑