Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,912,634 T→G T340P (ACC→CCC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,912,6340TG100.0% 400.8 / NA 106T340P (ACC→CCC) relA(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (50/56);  total (50/56)

Only 100 of 106 total aligned reads displayed.

GCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGTGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGC  >  NC_000913/2912489‑2912781
                                                                                                                                                 |                                                                                                                                                   
gCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCgg                                                                                                                                                 <  1:237439/150‑1 (MQ=255)
 ccATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGAt                                                                                                                                               <  2:42681/151‑1 (MQ=255)
 ccATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGAt                                                                                                                                               >  1:809281/1‑151 (MQ=255)
  cATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGAtt                                                                                                                                              >  2:393559/1‑151 (MQ=255)
     ctctTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTgg                                                                                                                                           >  1:956862/1‑151 (MQ=255)
       ctTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTgg                                                                                                                                           <  1:83870/149‑1 (MQ=255)
       ctTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTgg                                                                                                                                           <  1:561037/149‑1 (MQ=255)
       ctTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTg                                                                                                                                            <  3:587992/148‑1 (MQ=255)
       ctTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTg                                                                                                                                            <  1:465806/148‑1 (MQ=255)
        ttCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGAtc                                                                                                                                        >  4:40043/1‑151 (MQ=255)
        ttCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGAt                                                                                                                                         >  1:1123728/1‑150 (MQ=255)
         tCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGAtct                                                                                                                                       >  2:397969/1‑151 (MQ=255)
         tCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGAtct                                                                                                                                       >  1:890559/1‑151 (MQ=255)
             gCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAAc                                                                                                                                   <  3:321795/151‑1 (MQ=255)
              ccACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACg                                                                                                                                  <  3:673879/151‑1 (MQ=255)
              ccACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACg                                                                                                                                  >  3:709825/1‑151 (MQ=255)
                 cGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGtt                                                                                                                               <  1:1081001/151‑1 (MQ=255)
                  gCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGttt                                                                                                                              >  1:387783/1‑151 (MQ=255)
                     aTCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGttttt                                                                                                                            >  1:74322/1‑150 (MQ=255)
                     aTCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTc                                                                                                                           <  4:160542/151‑1 (MQ=255)
                       cAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTcc                                                                                                                          <  3:56313/150‑1 (MQ=255)
                       cAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCa                                                                                                                         <  2:533434/151‑1 (MQ=255)
                        aGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCa                                                                                                                         <  1:763814/150‑1 (MQ=255)
                           ttACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCAccc                                                                                                                      <  3:1078761/150‑1 (MQ=255)
                            tACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCgg                                                                                                                    >  1:1248457/1‑151 (MQ=255)
                            tACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCg                                                                                                                     <  1:1246723/150‑1 (MQ=255)
                             aCGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTCTTTCCACCCGGc                                                                                                                   <  3:882382/151‑1 (MQ=255)
                                cAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGcccc                                                                                                                >  3:203597/1‑151 (MQ=255)
                                  gCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAg                                                                                                              <  2:259449/151‑1 (MQ=255)
                                   ccAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGcccc                                                                                                                >  1:402667/1‑148 (MQ=255)
                                    cAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGa                                                                                                             >  1:388421/1‑150 (MQ=255)
                                    cAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGa                                                                                                             >  2:54252/1‑150 (MQ=255)
                                        cAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCAc                                                                                                        <  4:399117/151‑1 (MQ=255)
                                           tCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCa                                                                                                         <  4:88966/147‑1 (MQ=255)
                                           tCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACCCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACgg                                                                                                      <  1:525455/150‑1 (MQ=255)
                                               gTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGa                                                                                                 <  4:125775/151‑1 (MQ=255)
                                                   tCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAAt                                                                                               >  1:1312151/1‑149 (MQ=255)
                                                    cATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAg                                                                                             <  1:1302289/150‑1 (MQ=255)
                                                    cATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAg                                                                                             <  4:523353/150‑1 (MQ=255)
                                                     aTGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGa                                                                                            <  4:161609/150‑1 (MQ=255)
                                                     aTGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGAc                                                                                           >  1:1099089/1‑151 (MQ=255)
                                                      tGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGAc                                                                                           <  4:345814/150‑1 (MQ=255)
                                                         cccGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGa                                                                                        <  4:562729/150‑1 (MQ=255)
                                                           cGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATaa                                                                                     <  4:540370/151‑1 (MQ=255)
                                                            gAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAAc                                                                                    >  1:1419533/1‑151 (MQ=255)
                                                            gAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAAc                                                                                    >  4:237153/1‑151 (MQ=255)
                                                              aCGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCg                                                                                  <  3:64451/151‑1 (MQ=255)
                                                              aCGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCg                                                                                  <  1:602707/151‑1 (MQ=255)
                                                              aCGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCg                                                                                  <  1:821909/151‑1 (MQ=255)
                                                                gTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGtt                                                                                >  1:1113571/1‑151 (MQ=255)
                                                                gTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGtt                                                                                >  2:293871/1‑151 (MQ=255)
                                                                gTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGtt                                                                                <  3:661186/151‑1 (MQ=255)
                                                                  gcgcCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTg                                                                              >  1:307602/1‑151 (MQ=255)
                                                                     ccgccAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGtt                                                                           >  4:398027/1‑151 (MQ=255)
                                                                        ccAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTc                                                                         <  3:1106576/150‑1 (MQ=255)
                                                                        ccAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCg                                                                        <  3:612687/151‑1 (MQ=255)
                                                                         cagcagCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCgg                                                                       >  1:251532/1‑151 (MQ=255)
                                                                         cagcagCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGAATGAATAGACTGATAACCGTTTGGTTTCgg                                                                       >  1:569768/1‑151 (MQ=255)
                                                                          agcagcCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCggg                                                                      <  3:1060644/151‑1 (MQ=255)
                                                                          agcagcCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCggg                                                                      >  3:837748/1‑151 (MQ=255)
                                                                             agcCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTa                                                                   <  4:442375/151‑1 (MQ=255)
                                                                             acccGCACCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGCACCACGGTATGAATAGACTGATAACCGCTTGGTTTCGGGtt                                                                    >  4:222247/3‑150 (MQ=255)
                                                                              gcCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAg                                                                  <  1:920853/151‑1 (MQ=255)
                                                                              gcCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAg                                                                  <  2:534202/151‑1 (MQ=255)
                                                                               ccGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGc                                                                 <  3:1264558/151‑1 (MQ=255)
                                                                               ccGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGc                                                                 >  1:1127598/1‑151 (MQ=255)
                                                                                 gcgcCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCg                                                                >  2:106022/1‑150 (MQ=255)
                                                                                         tttATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCg                                                       >  3:524805/1‑151 (MQ=255)
                                                                                            atatTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCa                                                    <  1:744090/151‑1 (MQ=255)
                                                                                               tttCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAAc                                                 >  3:806089/1‑151 (MQ=255)
                                                                                                  ccAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTc                                               <  4:222098/150‑1 (MQ=255)
                                                                                                   cAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCAt                                             <  1:359254/151‑1 (MQ=255)
                                                                                                     gTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATcc                                           <  3:1327790/151‑1 (MQ=255)
                                                                                                      tGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCg                                          >  3:1055019/1‑151 (MQ=255)
                                                                                                          cagcaACACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAg                                      <  2:109281/151‑1 (MQ=255)
                                                                                                             caACACCCAACTCTGCATCTTCATGCATCTGTTTCGGGCGGATATGGATCTCAACAGCTTTTCCACCCGGCCACAGAACCACGGCATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGGCGAAATCGTCAAACTCATCCGGCAg                                      >  4:568347/1‑148 (MQ=255)
                                                                                                              aaCACCCAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTgg                                  <  2:474824/151‑1 (MQ=255)
                                                                                                                    cAACTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAAGCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTGAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGAt                              >  1:22462/1‑149 (MQ=255)
                                                                                                                     aaCTCTGCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATagt                           >  1:1305960/1‑151 (MQ=255)
                                                                                                                           gCATCTTCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCCCCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATagtgagt                       <  3:890678/149‑1 (MQ=255)
                                                                                                                                ttCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACt                >  4:29296/1‑151 (MQ=255)
                                                                                                                                ttCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACt                >  3:223538/1‑151 (MQ=255)
                                                                                                                                 tCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGc                   <  3:1355723/147‑1 (MQ=255)
                                                                                                                                 tCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCa                  <  2:202800/148‑1 (MQ=255)
                                                                                                                                 tCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCAc                 <  2:233320/149‑1 (MQ=255)
                                                                                                                                 tCATGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCAc                 <  3:387840/149‑1 (MQ=255)
                                                                                                                                  cacgCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACt                >  2:174248/4‑149 (MQ=255)
                                                                                                                                   aTGCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATc             >  2:285285/1‑151 (MQ=255)
                                                                                                                                     gCATCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATccc           <  2:200266/151‑1 (MQ=255)
                                                                                                                                        tCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATcccc          >  1:227762/1‑149 (MQ=255)
                                                                                                                                        tCTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATcccc          >  3:1027291/1‑149 (MQ=255)
                                                                                                                                         cTGTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGt       >  1:635371/1‑151 (MQ=255)
                                                                                                                                           gTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATCCCCa         <  3:743852/147‑1 (MQ=255)
                                                                                                                                           gTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATCCCCAg        <  2:320297/148‑1 (MQ=255)
                                                                                                                                           gTTTGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGc     >  4:32469/1‑151 (MQ=255)
                                                                                                                                            tttGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCg    >  2:166224/1‑151 (MQ=255)
                                                                                                                                             ttGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCgg   >  3:1092271/1‑151 (MQ=255)
                                                                                                                                             ttGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCg    <  3:809393/150‑1 (MQ=255)
                                                                                                                                             ttGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCg    <  1:308452/150‑1 (MQ=255)
                                                                                                                                              tGGGGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACGACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGc  >  4:564312/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCCATCTCTTCCTGCCACGCAATCAGTTTACGCAGCCAGGCAATCCGGTCTTCATGTCCCGAACGTGCGCCGCCAGCAGCCGCGCCCTCTTTATATTTCCAGTGCGCAGCAACACCCAACTCTGCATCTTCATGCATCTGTTTGGTGCGGATTTGGATCTCAACGGTTTTTCCACCCGGCCCCAGAACCACGGTATGAATAGACTGATAACCGTTTGGTTTCGGGTTAGCGACGTAATCGTCAAACTCATCCGGCAGGTGGCGATAGTGAGTGTGCACTATCCCCAGTGCGGC  >  NC_000913/2912489‑2912781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: