Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,078 T→G *106Y (TAA→TAC metJ ← transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,0780TG99.1% 443.3 / NA 105*106Y (TAA→TACmetJtranscriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (54/50);  total (54/51)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACA  >  NC_000913/4127931‑4128223
                                                                                                                                                   |                                                                                                                                                 
gACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAg                                                                                                                                                <  4:179165/151‑1 (MQ=255)
   aaCGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTAt                                                                                                                                             >  3:727855/1‑151 (MQ=255)
    aCGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTAtt                                                                                                                                            <  2:566810/151‑1 (MQ=255)
           cGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACg                                                                                                                                      <  3:381721/150‑1 (MQ=255)
           cGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGt                                                                                                                                     <  3:935844/151‑1 (MQ=255)
            gttgttTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGtc                                                                                                                                    >  3:580920/1‑151 (MQ=255)
              tgtcTATGCCGGATGCCGCATGAACGCCGTATCCACCCTACAAGCACGTACAAACTCAATAAATTGCAATATGACGTAGGCCTTATAAGCGTAGCGCATCAGGAGATTCCACTCCGAGCCGCTCTTTTTTGCTTTAGTATTCCCActttt                                                                                                                                   <  1:677022/150‑5 (MQ=255)
                tttATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCg                                                                                                                                >  3:1307518/1‑151 (MQ=255)
                   aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGt                                                                                                                             <  2:269751/151‑1 (MQ=255)
                   aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGt                                                                                                                             >  2:289327/1‑151 (MQ=255)
                   aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGt                                                                                                                             <  1:960288/151‑1 (MQ=255)
                   aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGt                                                                                                                             >  1:865930/1‑151 (MQ=255)
                     gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGtt                                                                                                                            <  1:63790/150‑1 (MQ=255)
                     gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGtt                                                                                                                            <  3:27453/150‑1 (MQ=255)
                     gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGtt                                                                                                                            <  4:102938/150‑1 (MQ=255)
                     gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGtt                                                                                                                            <  2:332813/150‑1 (MQ=255)
                       cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAAt                                                                                                                         >  4:214821/1‑151 (MQ=255)
                       cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAAt                                                                                                                         <  3:39838/151‑1 (MQ=255)
                        ggATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATc                                                                                                                        >  3:90047/1‑151 (MQ=255)
                          aTGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATccc                                                                                                                      <  1:590422/151‑1 (MQ=255)
                            gcggcgTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATcccc                                                                                                                     >  1:471969/1‑150 (MQ=255)
                             cggcgTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCAt                                                                                                                   <  3:130887/151‑1 (MQ=255)
                             cggcgTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCAt                                                                                                                   <  3:687559/151‑1 (MQ=255)
                                cgTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCAtctc                                                                                                                >  2:213413/1‑151 (MQ=255)
                                cgTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCAtctc                                                                                                                >  4:357874/1‑151 (MQ=255)
                                cgTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCAtctc                                                                                                                <  3:1017994/151‑1 (MQ=255)
                                cgTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCAtctc                                                                                                                >  1:446816/1‑151 (MQ=255)
                                   gAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACg                                                                                                             >  2:422161/1‑151 (MQ=255)
                                    aaCGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGc                                                                                                            >  1:266732/1‑151 (MQ=255)
                                      cGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCAt                                                                                                          <  1:1085647/151‑1 (MQ=255)
                                       gCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATg                                                                                                         >  4:394595/1‑151 (MQ=255)
                                            aTCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGAtctc                                                                                                    <  4:256084/151‑1 (MQ=255)
                                            aTCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGAtctc                                                                                                    >  1:374630/1‑151 (MQ=255)
                                            aTCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGAtctc                                                                                                    >  3:435170/1‑151 (MQ=255)
                                            aTCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGAtctc                                                                                                    <  1:449287/151‑1 (MQ=255)
                                               cGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCttt                                                                                                 <  1:926020/151‑1 (MQ=255)
                                                 gCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCCTAGCGCATCAGGCGATTCCACTCCGCGCCTCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTg                                                                                               <  2:119856/151‑1 (MQ=255)
                                                  ccTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGc                                                                                              <  1:1085583/151‑1 (MQ=255)
                                                    tACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCg                                                                                            >  3:964712/1‑151 (MQ=255)
                                                          ttCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCg                                                                                      >  1:96197/1‑151 (MQ=255)
                                                           tCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCgg                                                                                     >  3:815493/1‑151 (MQ=255)
                                                                cAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGAttt                                                                                >  3:699529/1‑151 (MQ=255)
                                                                cAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGAttt                                                                                <  1:309395/151‑1 (MQ=255)
                                                                 aaaTTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTtc                                                                               >  2:368411/1‑151 (MQ=255)
                                                                 aaaTTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTtc                                                                               <  3:245602/151‑1 (MQ=255)
                                                                  aaTTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTtcg                                                                              >  1:986833/1‑151 (MQ=255)
                                                                  aaTTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTtcg                                                                              >  3:375119/1‑151 (MQ=255)
                                                                   aTTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTtcgt                                                                             <  3:200031/151‑1 (MQ=255)
                                                                   aTTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTtcgt                                                                             <  3:95703/151‑1 (MQ=255)
                                                                     tCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTtcgtc                                                                            <  3:555871/150‑1 (MQ=255)
                                                                      cAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCACGCTTTtcgtcta                                                                          <  1:711620/151‑3 (MQ=39)
                                                                       aaTAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCt                                                                         >  3:572020/1‑151 (MQ=255)
                                                                          aaaTTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTgcg                                                                      >  1:821118/1‑151 (MQ=255)
                                                                          aaaTTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTgcg                                                                      >  1:1138683/1‑151 (MQ=255)
                                                                           aaTTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTgcgc                                                                     >  3:718854/1‑151 (MQ=255)
                                                                            aTTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGct                                                                    <  1:964581/151‑1 (MQ=255)
                                                                                 aaTATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCttt                                                                >  1:401066/1‑150 (MQ=255)
                                                                                 aaTATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTa                                                               <  1:807752/151‑1 (MQ=255)
                                                                                  atatGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTAc                                                              >  3:1317055/1‑151 (MQ=255)
                                                                                      gACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAg                                                          <  3:1164438/151‑1 (MQ=255)
                                                                                          tAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGc                                                            >  3:578233/1‑145 (MQ=255)
                                                                                           aGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGc                                                            >  3:371599/1‑144 (MQ=255)
                                                                                           aGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCg                                                      <  1:673408/150‑1 (MQ=255)
                                                                                              ccTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGcat                                                  <  3:908204/151‑1 (MQ=255)
                                                                                               cTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGcatc                                                 >  1:104810/1‑151 (MQ=255)
                                                                                               cTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGcat                                                  <  3:400140/150‑1 (MQ=255)
                                                                                                  aTAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCatcatc                                              >  4:344801/1‑151 (MQ=255)
                                                                                                  aTAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCatcatc                                              >  1:203045/1‑151 (MQ=255)
                                                                                                  aTAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCatcatc                                              >  1:402700/1‑151 (MQ=255)
                                                                                                    aaGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCg                                            <  3:613184/151‑1 (MQ=255)
                                                                                                    aaGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCg                                            >  4:489173/1‑151 (MQ=255)
                                                                                                    aaGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCg                                            >  4:544314/1‑151 (MQ=255)
                                                                                                     aGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCgg                                           <  3:641382/151‑1 (MQ=255)
                                                                                                         tAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCaa                                        >  3:581548/1‑150 (MQ=255)
                                                                                                           gcgcATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAgg                                     <  3:725457/151‑1 (MQ=255)
                                                                                                            cgcATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGt                                    >  1:187091/1‑151 (MQ=255)
                                                                                                            cgcATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGt                                    >  3:905779/1‑151 (MQ=255)
                                                                                                             gcATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGtt                                   <  3:373429/151‑1 (MQ=255)
                                                                                                               aTCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGc                                 <  2:177429/151‑1 (MQ=255)
                                                                                                                 cAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGcc                                <  1:392625/150‑1 (MQ=255)
                                                                                                                  aGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCg                              >  3:364306/1‑151 (MQ=255)
                                                                                                                  aGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCg                              >  3:457980/1‑151 (MQ=255)
                                                                                                                      gATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCCCGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTa                           <  1:1392272/150‑1 (MQ=255)
                                                                                                                      gATTCCACTCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTaa                          <  3:1202280/151‑1 (MQ=255)
                                                                                                                              tCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGc                  >  3:1023640/1‑151 (MQ=255)
                                                                                                                              tCCGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGc                  >  1:885629/1‑151 (MQ=255)
                                                                                                                               ccGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGc                  <  3:716930/150‑1 (MQ=255)
                                                                                                                               ccGCGCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCGTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCa                 <  1:1127391/151‑1 (MQ=255)
                                                                                                                                cgcgCCGCTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGc                  >  1:1354063/1‑149 (MQ=255)
                                                                                                                                  cgccgcTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGaa              <  2:401287/151‑1 (MQ=255)
                                                                                                                                  cgccgcTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGa               >  3:873617/1‑150 (MQ=255)
                                                                                                                                      gcTCTTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGc          >  1:1417322/1‑151 (MQ=255)
                                                                                                                                       ctctTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCt         >  3:214958/1‑151 (MQ=255)
                                                                                                                                       ctctTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCt         >  3:303761/1‑151 (MQ=255)
                                                                                                                                        tctTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCtt        >  3:1090697/1‑151 (MQ=255)
                                                                                                                                         ctTTTTTGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCt         <  1:722995/149‑1 (MQ=255)
                                                                                                                                          ttttttGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCg      >  4:311997/1‑151 (MQ=255)
                                                                                                                                            ttttGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGc     <  3:207454/150‑1 (MQ=255)
                                                                                                                                             tttGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGca    <  1:98451/150‑1 (MQ=255)
                                                                                                                                               tGCTGTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGcaca  <  1:520983/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACA  >  NC_000913/4127931‑4128223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: