Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,793,850	(C)6→7	coding (260/552 nt)	btuE ←	glutathione peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,793,844	1	.	C	95.2%	136.0 / ‑1.1	42	coding (266/552 nt)	btuE	glutathione peroxidase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (17/23);  total (18/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

ACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATT  >  NC_000913/1793695‑1793985                                                                                                                                                        |                                                                                                                                                aCAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑‑c                                                                                                                                               >  2:560493/1‑151 (MQ=255)  cAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑‑cc                                                                                                                                              >  2:640453/1‑151 (MQ=255)        tGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑‑ccc‑cccc                                                                                                                                        >  2:81619/1‑149 (MQ=255)          cACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑‑ccc‑ccccatg                                                                                                                                     <  1:666718/151‑5 (MQ=255)          cACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑‑ccc‑ccccatg                                                                                                                                     >  1:333971/1‑147 (MQ=255)          cACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑‑ccc‑ccccatg                                                                                                                                     >  2:458087/1‑147 (MQ=255)           aCGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑‑ccc‑ccccatgt                                                                                                                                    >  2:256230/1‑146 (MQ=255)           aCGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑‑ccc‑ccccatgt                                                                                                                                    >  1:672749/1‑146 (MQ=255)            cGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCAtgtg                                                                                                                                    <  1:345273/151‑1 (MQ=255)                 tttGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTggtggt                                                                                                                               >  1:141858/1‑151 (MQ=255)                    gCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑ACCCCC‑CCCATGTGGTGGTAc                                                                                                                             <  1:67011/151‑1 (MQ=255)                     cTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTAc                                                                                                                             >  2:433179/1‑149 (MQ=255)                        aCCGTACGGGCATAGAATCAGGTCTATTCCGGCGCGACGGCGGTCGGCACTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCAGTTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTagag                                                                                                                           <  1:146388/148‑1 (MQ=255)                         ccATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTa                                                                                                                         >  1:502786/1‑149 (MQ=255)                          cATGCGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGGC‑AC‑CCC‑CCCATGTGGTGGGACAGGAAGt                                                                                                                      <  1:22556/151‑1 (MQ=255)                              cGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCATCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTa                                                                                                                  <  1:631951/151‑1 (MQ=255)                                        aTCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCAt                                                                                                        <  2:239567/151‑1 (MQ=255)                                        aTCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCAt                                                                                                        >  2:207463/1‑151 (MQ=255)                                                   tCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGccc                                                                                                <  1:593199/148‑1 (MQ=255)                                                    ccGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAATATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGtt                                                                                            <  1:521293/151‑1 (MQ=255)                                                           gACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTc                                                                                     >  2:571512/1‑151 (MQ=255)                                                                      gcgcTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGt                                                                          <  2:141862/151‑1 (MQ=255)                                                                      gcgcTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGt                                                                          >  2:116932/1‑151 (MQ=255)                                                                          tGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGc                                                                       >  2:308544/1‑150 (MQ=255)                                                                               caatcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGa                                                                 <  1:81619/151‑1 (MQ=255)                                                                                   caatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATcc                                                             <  1:39048/151‑1 (MQ=255)                                                                                     atTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATGCCa                                                           <  1:206390/151‑1 (MQ=255)                                                                                        tttGATACAGCGGACGGCGTACTTCGCCATTATCTTCAATCTTACTGTACATCGGGCCCGTC‑AC‑CCC‑CCCATGTGGTGGTACGGTAAGTTGTAATCTCTGCACCGCTGCCCGGTTCTTGTTCCAGAAGCTGGTTGCACGGGAATCCCAGc                                                         <  1:472281/150‑1 (MQ=255)                                                                                             tACATCGGATAGCGTCCTTCGCCATTAGCTTCAAACTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCAt                                                    <  1:367032/150‑1 (MQ=255)                                                                                             tACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCAt                                                    >  1:246487/1‑150 (MQ=255)                                                                                                     aTGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcg                                           <  1:629913/151‑1 (MQ=255)                                                                                                       ggCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                                         >  1:174291/1‑151 (MQ=255)                                                                                                         cGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgatcg                                       >  2:452368/1‑151 (MQ=255)                                                                                                          gTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgatcg                                       >  1:387239/1‑150 (MQ=255)                                                                                                           tCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGAc                                     >  1:577671/1‑151 (MQ=255)                                                                                                               tCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGAccc                                   >  2:123779/1‑149 (MQ=255)                                                                                                                 gCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGc                               <  2:425675/151‑1 (MQ=255)                                                                                                                  ccATTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCt                              >  1:97786/1‑151 (MQ=255)                                                                                                                    aTTAACTTCAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCttt                            >  1:206819/1‑151 (MQ=255)                                                                                                                            cAATCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAata                     <  2:512218/150‑1 (MQ=255)                                                                                                                             aaTCTTACTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATAtt                   <  2:81490/151‑1 (MQ=255)                                                                                                                                  tACTGATCATCGGTAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGTTTCTTTTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAGCCGCGAGCGACCCAGGCTTTCTGAATATTCTcc               <  1:388163/150‑1 (MQ=255)                                                                                                                                   aCTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCa              <  2:382666/150‑1 (MQ=255)                                                                                                                                   aCTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCa              <  1:371342/150‑1 (MQ=255)                                                                                                                                    cTGAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAAc            >  2:594676/1‑151 (MQ=255)                                                                                                                                    cTGAACATCGGGAACGTC‑A‑‑CCCACCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCaa             >  1:99631/1‑150 (MQ=255)                                                                                                                                      gAACATCGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACt           <  2:83869/150‑1 (MQ=255)                                                                                                                                        aCATCGGGAACGTCAA‑‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCACTTCACCGCCGCCAGGTACTTGTTCCAGAAACTCGTTGCACCGGAATCCCAGCTCCTTAAACCCTCTATCGACCCAGGCTTTCTGAAGCTCCACCACCTGCt        <  1:199897/151‑1 (MQ=255)                                                                                                                                            cGGGAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCata    <  2:170915/151‑1 (MQ=255)                                                                                                                                              ggAACGTC‑AC‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATAtt  >  2:258877/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                ACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTC‑A‑‑CCC‑CCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATT  >  NC_000913/1793695‑1793985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑