Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,143,684	(G)6→7	coding (419/3318 nt)	yegE →	putative diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,143,678	1	.	G	97.4%	131.4 / ‑3.4	38	coding (413/3318 nt)	yegE	putative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (16/21);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CGCTGAACATGACCTGGACGACCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TT‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGCGCCAT  >  NC_000913/2143528‑2143820                                                                                                                                                         |                                                                                                                                               cGCTGAACATGACCTGGACGACCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑tt                                                                                                                                                 <  2:80451/151‑1 (MQ=255)        cATGACCTGGACGACCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TT‑ggggggg                                                                                                                                         >  1:408598/1‑150 (MQ=255)          tGACCTGGACGACCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TT‑gggggggtg                                                                                                                                       >  2:213532/1‑148 (MQ=255)                 gacgacCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGt                                                                                                                                 >  2:247417/1‑151 (MQ=255)                  acgacCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGtt                                                                                                                                <  2:596681/151‑1 (MQ=255)                    gacCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGt                                                                                                                              >  2:60874/1‑151 (MQ=255)                              atTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACg                                                                                                                    <  2:333333/151‑1 (MQ=255)                                       gCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGAc                                                                                                           <  2:662836/151‑1 (MQ=255)                                          gTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGAt                                                                                                        <  2:522224/151‑1 (MQ=255)                                                ggggCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCtctc                                                                                                  >  2:142585/1‑151 (MQ=255)                                                            ctACGTAAATTGCAGCCGTGGTATACCCCCTTGCAAAATCTGGCAGACTCGCTGCGTCTGGCGTTTGGCATGGCCAATGTTCCGCCTCTGCTG‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCAtt                                                                                          <  1:359043/147‑1 (MQ=255)                                                               cGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGa                                                                                     <  1:49261/149‑1 (MQ=255)                                                                gTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGatat                                                                                  >  2:152686/1‑151 (MQ=255)                                                                      tgctgcCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTAc                                                                            >  1:110969/1‑151 (MQ=255)                                                                          gcCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGt                                                                         <  2:275215/150‑1 (MQ=255)                                                                              tGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGa                                                                     >  2:401048/1‑150 (MQ=255)                                                                               ggTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAAt                                                                   <  2:299137/151‑1 (MQ=255)                                                                               ggTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAAt                                                                   <  1:213643/151‑1 (MQ=255)                                                                                gTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATc                                                                  >  1:57418/1‑151 (MQ=255)                                                                                gTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATc                                                                  <  2:671498/151‑1 (MQ=255)                                                                                   taACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCAt                                                               >  1:84761/1‑151 (MQ=255)                                                                                    agccccGTGCAAAATCTGGCTGACTGGCTGCGTCTGGCTCTCGGCAGCGCCATTGTTCCGCCTCTC‑TTGGGGGGGTGTTCTGTTTGTCCTGCTGACGCCCGGAGACGATCCTCTCGCGGCATTTTCGATAAGGGCACTGTCCGAATCCa                                                                <  1:19285/147‑1 (MQ=255)                                                                                       cccTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGgc                                                           <  2:622087/151‑1 (MQ=255)                                                                                          ttGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGct                                                        >  2:415943/1‑151 (MQ=255)                                                                                              aaaaTCTGGCTGACTGGCTGCGTCTGGCACTCGGCTGCGCCATTGGTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTg                                                     <  1:467769/150‑1 (MQ=255)                                                                                               aaaTCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGc                                                   <  2:683228/151‑1 (MQ=255)                                                                                               aaaTCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGc                                                   <  2:201764/151‑1 (MQ=255)                                                                                               aaaTCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGc                                                   >  1:615993/1‑151 (MQ=255)                                                                                                     ggCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGt                                             <  2:20821/151‑1 (MQ=255)                                                                                                         gACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGc                                           <  1:665338/149‑1 (MQ=255)                                                                                                             ggCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTg                                      <  1:362492/150‑1 (MQ=255)                                                                                                                          aCTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTaa                        <  2:431121/151‑1 (MQ=255)                                                                                                                          aCTCGGCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTa                         >  1:318997/1‑150 (MQ=255)                                                                                                                              ggCAGCGCCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAAc                      <  1:358238/149‑1 (MQ=255)                                                                                                                                   cgcCATTGTTCCGCCTCTG‑TTGGGGGGGTGTTCTGCTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACCGGTGCCGCTGGGATTGTTATTTAAACCACACt                >  1:297841/1‑150 (MQ=255)                                                                                                                                     ccATTGTTCCGCATCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGAGCTCTGGCCCTGGTGCCGCTGGGATTGTTCTTTAAACCACATTa               >  1:623970/1‑149 (MQ=255)                                                                                                                                       aTTGTTCCGCCTCTG‑TTGGGGGGGCGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATctg           >  2:146275/1‑151 (MQ=255)                                                                                                                                           ttCCGCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCtgctgc       >  2:605291/1‑151 (MQ=255)                                                                                                                                               gCCTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGCGCCa   <  2:538976/151‑1 (MQ=255)                                                                                                                                                ccTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGCGCCa   <  2:212186/150‑1 (MQ=255)                                                                                                                                                ccTCTG‑TTGGGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGCGCCAt  >  2:331290/1‑151 (MQ=255)                                                                                                                                                         |                                                                                                                                               CGCTGAACATGACCTGGACGACCATCAATATTGTTGAAGCCGTGGTCGGGGCAGTGCTGCTACGTAAATTGCTGCCGTGGTATAACCCCTTGCAAAATCTGGCTGACTGGCTGCGTCTGGCACTCGGCAGCGCCATTGTTCCGCCTCTG‑TT‑GGGGGGTGTTCTGGTTGTCCTGCTGACGCCCGGAGACGATCCTCTCAGGGCATTTTTGATATGGGTACTGTCAGAATCCATCGGCGCTCTGGCACTGGTGCCGCTGGGATTGTTATTTAAACCACACTATCTGCTGCGCCAT  >  NC_000913/2143528‑2143820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑