| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,890 | 0 | C | A | 58.3% | ‑4.8 / 25.5 | 25 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (7/3); new base A (0/14); total (7/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.47e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-02 | |||||||||||
CTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTAT‑CCAACGCCATGTGCAAAAATGC‑CTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGA‑AAGGGAAGTGCGGCGAATCAGCTCCAGAACCGC > NC_000913/357740‑358015 | cTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTAT‑CCAACGCCATGTGCAAAAATGC‑CTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaac > 2:30893/1‑151 (MQ=255) tAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTAT‑CCAACGCCATGTGCAAAAATGC‑CTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaa > 1:67965/1‑150 (MQ=255) cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTCCCGTGATTCATTCCCGCCAACAACCGCGCATTTAT‑CCAACGCCATGTGCAAAAATGC‑CTT‑G‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑ccgccg < 2:255887/148‑1 (MQ=255) gCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTAT‑CCAACGCCATGTGCAAAAATGC‑CTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCagt > 2:37167/1‑151 (MQ=255) cAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTAT‑CCAACGCCATGTGCAAAAATGC‑CTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCagt > 2:197865/1‑150 (MQ=255) tcccAGCCGCAGCCTTGTGTCAGGGGCTCCCGTGTTTCTTTCCCCCCAACACCCGCGCTTTTAC‑CAACCGCCTTGTGAAAAAATGC‑TTC‑C‑GCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAA‑CCGCCGTTCTCTCCagtagt < 2:124397/148‑1 (MQ=255) cagccttgtgtgGGCGGTTCCCGTTTTTCTTTCCCCCCAACACCCGCCCTTTTAC‑CCACCCCCGTGTAAAAAATTCC‑CTT‑C‑GCGGCGCCTGCCGCCCGGCTTTTTTCCCGCCGAAACAAAA‑CCGCCGTTCTCTCCAGTAGTgg < 1:428036/137‑1 (MQ=255) aGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTAT‑CCAACGCCATGTGCAAAAATGC‑CTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCggggg > 2:396836/1‑147 (MQ=255) aGACCTGTGTCAGCGGCTACCGAGATTCATTTCCGCCAACAACCGCGCATTTAT‑CCAACGCCATGTGCAAAAATGC‑CTT‑A‑GCGGCGGCTGTCTGCCAGCTATTTTTACGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTCGCgggggc > 1:598196/1‑147 (MQ=255) tgtgTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTAT‑CCAACGCCATGTGCAAAAATGC‑CTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGcgaaaa > 2:613362/1‑143 (MQ=255) tgccgggggCTCCGGTGATCCATTCCCCCCAACACCCGCGCTTTTAC‑CCAACGCAATGTG‑AAAAATGCTCTC‑G‑GGGGGGGCTTTCTGCCAGCTTTTTTCCCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCa < 1:417383/135‑1 (MQ=255) gtCGGCGTCTCCCTTCATTCTTTCCCCCCAACAGCCCCTATTTTAT‑CCACCCCCATGTGAAAAATGGC‑TTT‑G‑GCGGGGTCTGTTGCCCACCTATTTTCCCGCCGAAAAAAAC‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGaaa < 1:457894/148‑1 (MQ=255) ggcgaccccGTGTTTCATTCCCCCCAA‑AACCCCGCCTTTTTACCAACCCCGTTTGCAAAAATGC‑CTTCC‑GGGGGGGTTGTC‑GCCAGGTTTTTTCCCCCCCAAAAAAAACCCGCCGTTCTC‑CCAGTAGTGGCGGGGCAAGAGAAATa < 1:297578/139‑1 (MQ=255) gcgcccaCCGTGTTTCTTCCCCCCCACCACCCGCCCTTTTAC‑CCACCCCAATGCAAAAAAATCC‑CTT‑G‑GGGGCGGTTGTCGCCCACATATTTTCCCGCCAAAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCttt < 1:197888/145‑1 (MQ=255) cgttttcctttcccGCCAACAACCCCGCATTTCC‑CCACCGCCATTTAAAAAAACGC‑CTC‑C‑GCGGCGGCTGTTGCCCAGCTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCttt < 2:430524/131‑1 (MQ=255) cccccAACACCCGCATATTTAC‑CAAACGCCGTGTAAAAAATTGC‑CTC‑C‑GGGGGGGCGTTCTGCCAGTTTTTTTCCCGCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTtgtgt < 1:600227/146‑1 (MQ=255) cacccGCGCTTTTCC‑CCACCGCAATGTGAAAAATTCC‑TTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATc < 2:372424/146‑1 (MQ=255) ttttccccacccccttgtaCAAAAATCC‑TTG‑C‑GGGGCGGTTGTCTCCCGGTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTa < 1:445186/135‑1 (MQ=255) cGCCATG‑GAAAAAAAGC‑CCT‑CGGGGGGGGCTGTCGCCCAGTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACaa < 1:647701/144‑1 (MQ=255) gtgaaaaaaGC‑CTTCC‑GCGGCGGCTGTCGCCCAGTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGAGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtg < 1:520963/137‑1 (MQ=255) aaaaTGC‑CTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAAATTTTAACCCCCCCTGTTTTGTGGGGAACGCTGCTGGGGAAAAAGAGGAGAAGGGAAgggg > 2:601853/1‑148 (MQ=255) cggggcggcTGTCTGCCATCTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGA‑AAGGGAAGTGCGGCGAATCa < 2:655303/146‑1 (MQ=255) tccctttactttccccgcagCAAGAAAT‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGA‑AAGGGAAGTGCGGCGAATCAGCTCCAGAAcc < 1:354039/133‑1 (MQ=255) cccAGTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGA‑AAGGGAAGTGCGGCGAATCAGCTCCAGAACcgc < 2:665120/147‑1 (MQ=255) cggCTTTTTTCCCCCCACAAAAAAC‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGA‑AAGGGAAGTGCGGCGAATCAGCTCCAGAAcc < 1:612055/142‑1 (MQ=255) ggtttttttcCCGCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGA‑AAGGGAAGTGCGGCGAATCAGCTCCAGa < 1:345561/135‑1 (MQ=255) | CTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTAT‑CCAACGCCATGTGCAAAAATGC‑CTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGA‑AAGGGAAGTGCGGCGAATCAGCTCCAGAACCGC > NC_000913/357740‑358015 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |