Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913501,5750AC51.5% 40.3 / 53.5 33V555G (GTG→GGG) ybaLinner membrane putative NAD(P)‑binding transporter
Reads supporting (aligned to +/- strand):  ref base A (14/2);  new base C (0/17);  total (14/19)
Fisher's exact test for biased strand distribution p-value = 1.47e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-06

TTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCC  >  NC_000913/501426‑501718
                                                                                                                                                     |                                                                                                                                               
ttAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcaccac                                                                                                                                                >  1:435857/1‑151 (MQ=255)
   aGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTc                                                                                                                                             >  2:14397/1‑151 (MQ=255)
         cgtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccg                                                                                                                                        <  2:382005/150‑1 (MQ=255)
                           aTAGGGCGTCCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCGGGCGCGTCTTATCATGCCTACATTTTTTTCATATTTTACATCCGGCAACCCCCGTTTCCCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCcagcag                                                                                                                     <  1:244899/151‑1 (MQ=38)
                                   ttCCCGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGCCGCTGGCGCGTCTTATCATGCCTACATTTTTTTCATTTTTTACACCCGGCAACCACCGTTTCCCCCGTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAg                                                                                                               <  2:541013/149‑1 (MQ=38)
                                              tCTGGTTTTTGGCTCTCGATCCCTGATGCGCCGCGGGCGCTTTTTATCATGCCTATATTTTTTTCTTTTTTTACACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTAc                                                                                                       <  1:45012/146‑1 (MQ=255)
                                                 ggCTTTTGGCTCTCGATGCCTGATGCGCCGCTGGCGCTTCTTATCATCCCTACATATTTTTCATATTTTACATCCGGCAACCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACggg                                                                                                    <  2:388376/146‑1 (MQ=255)
                                                        gcctctCGATGCCTGAGGCGCCGTTGGGGCGTTTTATCATCCCTACATTTTTTCCATTTTTTACATCGGGCACCCCCCTTTACCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                                                                         <  1:99761/148‑1 (MQ=255)
                                                          ctctCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCg                                                                                      >  1:7840/1‑151 (MQ=255)
                                                               gATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCAt                                                                                 >  2:264053/1‑151 (MQ=255)
                                                                   ccTGATGCGCCGCTGGCGCGTCTTATCATGCCTACATTTTTTTCATATTTTCCATCCGGCAACCACCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATc                                                                                <  2:319371/148‑1 (MQ=255)
                                                                     tGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                                                           >  2:22422/1‑151 (MQ=255)
                                                                                 ggCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTCCCCCGTCACCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGca                                                                 <  2:556907/149‑1 (MQ=255)
                                                                                      gttttATCTTGCCTACATATTTTTCATTTTTTACATCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATCCGCACCACGt                                                           <  1:325921/147‑1 (MQ=255)
                                                                                      gttttATCATGCCTACATATTTTTCATTTTTAACATCCGGCACCCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGt                                                           <  2:75967/147‑1 (MQ=255)
                                                                                            tCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTg                                                     >  1:115639/1‑150 (MQ=255)
                                                                                                 ccactttttttttAATTTTTTACATCCGGCAACCCCCTTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGat                                                   <  1:565682/139‑1 (MQ=255)
                                                                                                   tACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGc                                             >  2:448673/1‑151 (MQ=255)
                                                                                                       tatTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACt                                         >  2:138406/1‑151 (MQ=255)
                                                                                                          ttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTc                                       >  2:573108/1‑150 (MQ=255)
                                                                                                          ttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTc                                       >  2:318415/1‑150 (MQ=255)
                                                                                                          ttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTc                                       >  1:267245/1‑150 (MQ=255)
                                                                                                            ttCATATTTTCCACCCGGCACCCACCTTTTCCCCCGCCCCCACCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCAt                                     <  1:106500/150‑1 (MQ=255)
                                                                                                            ttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATc                                    >  2:550699/1‑151 (MQ=255)
                                                                                                                     tACATCCGGCAACCACCGTTTGCCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATg                           >  1:684739/1‑151 (MQ=255)
                                                                                                                          ccGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGgcg                       <  2:326751/150‑1 (MQ=255)
                                                                                                                              caccccccttttCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCggg                   <  1:317301/141‑1 (MQ=255)
                                                                                                                               accccccttttCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCa                 <  2:174670/143‑1 (MQ=255)
                                                                                                                                 cccccGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaat               <  2:630151/148‑1 (MQ=255)
                                                                                                                                    ccGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaat               <  2:305369/148‑1 (MQ=255)
                                                                                                                                    ccGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaataat            >  2:293608/1‑151 (MQ=255)
                                                                                                                                           ccccGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAat      <  2:224700/150‑1 (MQ=255)
                                                                                                                                           ccccGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCa        <  1:644932/148‑1 (MQ=255)
                                                                                                                                              cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATcc  >  1:134717/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
TTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCC  >  NC_000913/501426‑501718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑