Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,412,8980CT40.5% 36.3 / 30.1 42A190T (GCC→ACC) acrSacrAB operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (22/3);  new base T (0/17);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 4.48e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACTAAAACTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGG  >  NC_000913/3412750‑3413046
                                                                                                                                                    |                                                                                                                                                    
ccACTAAAACTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCt                                                                                                                                                     <  1:46132/150‑1 (MQ=255)
ccACTAAAACTGGCTAACTGTGACGAACTGAATTTTCAGGACAGAATGCGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCtt                                                                                                                                                    >  1:153439/1‑151 (MQ=255)
 cACTAAAACTGGTTAACTGTGACGAACTGAATTTTCAGGACAGCATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTg                                                                                                                                                   >  1:473174/1‑151 (MQ=255)
     aaaaCTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACGCTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTGTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTta                                                                                                                                               <  2:469738/151‑1 (MQ=255)
            gTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGAt                                                                                                                                        <  1:539920/151‑1 (MQ=255)
                               aTTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCAt                                                                                                                     >  2:576046/1‑151 (MQ=255)
                                        aCAGAATGTGATTTTACATGACATTTAATTCATTCTTTTGATAAATTATTTTCTTTATTTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGGGGGGGGGTTTTTTTAAAAAGATCATACCCCGCCATATTCATTAACCAGt                                                                                                             <  2:74525/150‑1 (MQ=255)
                                          aaaaTGTAATTTTACATGACATTTATTTCTTTCTTTTGATAAATTATTTTCGTTTTGTTTTCATCTGGAATAAACTTTTTTAATACTTTATCGCCCAGGGGGGGGGTTTGTTTAAAAAAATCAAACCCCGCCATATTCATTAACCAGtttt                                                                                                          <  2:245005/149‑1 (MQ=255)
                                           gAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTg                                                                                                         >  1:38118/1‑151 (MQ=255)
                                            aaTGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTg                                                                                                         >  2:331682/1‑150 (MQ=255)
                                            aaTGTAATTTTACATGACTTTTAATTCTTTCTTTTGATGAATTATTTTGTTTATTTTTTCATTTGGTATAAAAATTTTTAATACGTTATCGACAAGAGGGGGGTTTTGTTTATAAAAATCATACCCCGCCATATTCATTAACCAGttt                                                                                                           <  1:439513/148‑1 (MQ=255)
                                              ttttattttACATGTCACTTATTTCTTTTTTTTAATAATTTTATTTCTTTATTTTTTCTTTTGATATAATTTTTTTTAATCCTTTTTCGCCCAGAGGGGGGGTTTTTTAAAAAAAATCATATCCCGCCATATTCATTAACCAGtttt                                                                                                          <  1:139335/141‑1 (MQ=255)
                                                tgAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACa                                                                                                     >  2:235635/1‑150 (MQ=255)
                                                 gAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAAt                                                                                                   >  1:17255/1‑151 (MQ=255)
                                                             cacTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGc                                                                                       >  2:72696/1‑151 (MQ=255)
                                                               cTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCAc                                                                                     >  1:160561/1‑151 (MQ=255)
                                                                      cattcGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCaat                                                                              >  2:106668/1‑151 (MQ=255)
                                                                            tttGATAAATTATTTTCGTTATTTTTTAATCGGCAATAAACATTTTTAATACTTAATCGCCCAGGGGGGGGTTTTTTTTATAAAGATCAAAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCaataat                                                                           <  2:467042/148‑1 (MQ=255)
                                                                                   aattaattTCGTTATTTTTTCATCTGGAATGAACATTCTTAATACGTTACCGACCAGGGCGGGGGTTTTTTTATAAAGATCAAAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAatcat                                                                     <  2:17255/147‑1 (MQ=255)
                                                                                     ttatttTCTTTTTTTTTTCATCTGCCATAAACTTTCTTAATCTTTTATCGCCCAGGGGGGGGTTTTTTTTATAAAGATAAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCaatggtgccttcagc                                                                  <  1:413742/148‑13 (MQ=255)
                                                                                     ttaattTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCacaac                                                               >  2:637588/1‑151 (MQ=255)
                                                                                       attttCTTTTTTTTTTCATCTGGCTTGAACTTTCTTAATTCTTTACCGCCCAGGGCGGGGGTTTTTTTAAAAAGATAATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCacaaca                                                              <  2:245094/148‑1 (MQ=255)
                                                                                       aattTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACAt                                                             >  2:192641/1‑151 (MQ=255)
                                                                                         tttCTTTTTTTTTTCATCTGGAAAAAACATTTTTAATCCTTTACCGACCAGGGGGGGGGTTTTTTAAAAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATc                                                            <  2:502943/150‑1 (MQ=255)
                                                                                              ttATTTTTTCATCTGGAATAAACATTCTTAATACGTTATCGACAAGGGGGGGGGTTTTTTTATAAAGATAATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTaaa                                                        <  2:96878/149‑1 (MQ=255)
                                                                                               ttttttttCATCTGCCATAAACATTCTTAATCCGTAATCCACAAGAGCGGGGTTTTTTTTAAAAAGATAAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAAt                                                       <  2:297846/145‑1 (MQ=255)
                                                                                                   ttttCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGt                                                 >  1:376519/1‑151 (MQ=255)
                                                                                                     ttCATCTGGCATGAACATTATTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGAACATAACCCGCCATATTCATTAACCAGGTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGtt                                                >  1:562270/1‑150 (MQ=255)
                                                                                                        aTCTGGCATAAACATTCTTAATACTTTATCGACCAGGGGGGGGGTTTTTTTAAAAAGATCATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTAt                                              <  2:332364/149‑1 (MQ=255)
                                                                                                          cTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGc                                          >  2:136119/1‑151 (MQ=255)
                                                                                                           tGGAATAAACTTTTTTAATACTTTACCGACCAGGGCGGGGTTTTTTTTAAAAAAATAATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTAtt                                             <  2:496116/147‑1 (MQ=255)
                                                                                                               ataaaCATTTTTAATCCGTTATCGACAAGGGCGGGGGTTTTTTTAAAAAGATAAAACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTa                                        <  1:370881/145‑1 (MQ=255)
                                                                                                               aTGAACATTTTTAATACTTTACCGACCAGGGGGGGGGTTTTTTTAAAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTacac                                     <  2:69885/151‑1 (MQ=255)
                                                                                                                  aaCATTTTTAATACGTTACCCACGAGGGGGGGGGTTTTTTTAAAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACaa                                   <  2:602457/150‑1 (MQ=255)
                                                                                                                   aCATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAAcc                                 >  1:312147/1‑151 (MQ=255)
                                                                                                                              aCGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGac                      >  1:332694/1‑151 (MQ=255)
                                                                                                                                    tCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCt                >  1:445153/1‑151 (MQ=255)
                                                                                                                                     cGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTg               >  1:5161/1‑151 (MQ=255)
                                                                                                                                     cGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTg               >  2:647620/1‑151 (MQ=255)
                                                                                                                                           gagCGGTGTTTTTTTTATAAAAAACATACCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATa          <  1:331005/150‑1 (MQ=255)
                                                                                                                                               gggggCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTcg     >  1:583867/1‑151 (MQ=255)
                                                                                                                                                   gCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCgg  >  1:252482/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCACTAAAACTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTATAAAGATCATAACCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGG  >  NC_000913/3412750‑3413046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑