Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,0610CT39.2% 62.5 / 17.9 51E136K (GAA→AAA) plsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (9/22);  new base T (20/0);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.68e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCG  >  NC_000913/4255912‑4256206
                                                                                                                                                     |                                                                                                                                                 
aGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCa                                                                                                                                                  <  2:364205/151‑1 (MQ=255)
        ccgtgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCgg                                                                                                                                          <  1:267724/151‑1 (MQ=255)
          gtgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGgcg                                                                                                                                        <  2:286911/151‑1 (MQ=255)
             ccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTAcccccc                                                                                                                                            >  1:661766/1‑144 (MQ=255)
             ccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGgcgcgc                                                                                                                                     <  1:537106/151‑1 (MQ=255)
             ccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCCTTTTTAcccccc                                                                                                                                            >  1:610370/1‑144 (MQ=255)
                    cATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTACTTCGCCCTTTTTACCCCCCGGCGCGCGAcc                                                                                                                                 >  1:278708/1‑148 (MQ=255)
                         gCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACGCCCCCGCGCGCGGCCAAACa                                                                                                                            >  2:299087/1‑148 (MQ=255)
                           cATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCCGCGCGCGACCCAACATCAcc                                                                                                                       >  2:480555/1‑151 (MQ=255)
                               cGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACCCCCCGGCGCGCGACCCAAAATCaaccac                                                                                                                    >  2:405859/1‑145 (MQ=255)
                                      gCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCa                                                                                                             <  1:51804/150‑1 (MQ=255)
                                        gAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCCGCGCGCGACCCAACATCACCGACACCGGCAcc                                                                                                           >  2:439788/1‑150 (MQ=255)
                                            cTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCGCCTGGCCTTTTTTACGCCCCGGGGCGCGCCCAAACATTAACGACCATGGAACCa                                                                                                          >  2:571549/1‑147 (MQ=255)
                                            cTGACGGCGAGAAACGCAAAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCt                                                                                                       <  1:333943/150‑1 (MQ=255)
                                             tGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTACTTTGCCTTTTTTACGCCCCGGCGCGCGAACAAAAATCACCGACACTGGCACCATCt                                                                                                       >  2:505754/1‑149 (MQ=255)
                                              gACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTAGCCTTTTTTACGCCCCGGCGCGGGGCCAAAAAACACCGACACTGGGAACAtat                                                                                                       >  2:78063/1‑146 (MQ=255)
                                                       aaaCGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAt                                                                                           <  1:657899/151‑1 (MQ=255)
                                                           gCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTgg                                                                                       <  1:207661/151‑1 (MQ=255)
                                                                    tGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACCTCGCCCTTTTTACGCCCCCGCGCGCGACCAAACAACACCACCACTGGCACCATCTTCACATCCCAATTTGGGTTGCTAc                                                                               >  1:103748/1‑150 (MQ=255)
                                                                     gTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACg                                                                              <  2:42874/150‑1 (MQ=255)
                                                                      tCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACGCCCCGGCGCCGCACCCAAAAACACCGACCCTGGCCCCATCTTCACCTCCAGATTTtgggt                                                                                    >  1:331007/1‑141 (MQ=255)
                                                                          gACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCa                                                                         <  2:496901/150‑1 (MQ=255)
                                                                             cGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTACATCCCCTTTTTTACGCCCCCGCCCGCGACCCAACAACACCGACACCGGGACCAACTGCACATCCCAATTTGGGGTGCTACGGTGCaaa                                                                       >  1:49374/1‑149 (MQ=255)
                                                                              gAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACCCCCCCGCGCGCGACCAAAAATCACCGACACTGGCACCCTCTGCACATCCCGATTTTGGTTGCTACGGGGCAAAACg                                                                    >  2:190822/1‑151 (MQ=255)
                                                                               aGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCga                                                                   <  2:207714/151‑1 (MQ=255)
                                                                                  cacaGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTTCTTCTCCTTTTTTACGCCCCCGCGCGCGCACAAACAACACCGGCACGCGCATCCCCTGCGCAACCCTATTTTGGTTGCTACCGCTCAAATCgcg                                                                  >  2:40236/1‑147 (MQ=255)
                                                                                   acaGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACCCCCCCGCGCGCGACCCAACAACACCCACACTCGCACCATCCGCACATCCCGATTTTGGGTTCTACGGTGCAAATCgag                                                                  >  1:666193/1‑148 (MQ=255)
                                                                                     aGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGt                                                             <  2:468889/151‑1 (MQ=255)
                                                                                      gTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTc                                                            <  2:650240/151‑1 (MQ=255)
                                                                                          aGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACCCCCCCGCGCGCGACCAAACATCACCGACACTGGCACCCTCTGCACATCCAGATTTTGGTTGCTACGGTGCAAATCGAGATAGTCGTgg                                                        >  2:161840/1‑151 (MQ=255)
                                                                                           gCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGa                                                       <  2:215675/151‑1 (MQ=255)
                                                                                           gCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCCCCTTTTTCACGCCCCCGCGCGCGACCAAACAACACCGAAACTGGCACCATCTGCACATCCAGATTTTGGTTGCTACGGTGCAAATCGAGATAGTCGTg                                                         >  2:465342/1‑149 (MQ=255)
                                                                                             gAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCCGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCCGATTTTGGTTGCTACGGTGCAAATCGAGATAGTCGTGGaa                                                      >  2:499902/1‑150 (MQ=255)
                                                                                              aaaaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACa                                                    <  1:598109/151‑1 (MQ=255)
                                                                                                aaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCCTTTTCACGCCCCGGCGCGCGAACAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTTGGTTTCTACCGTGGAAAACGGGATAGTTGTGGAACAGc                                                  >  2:488505/1‑151 (MQ=255)
                                                                                                            gCCGTTAAGCATACGCAGCGGCGGGTTCACTTTGCCTTTTTTACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCCTCTGCACATCCAGATTTTGGTTGCTACCGTGCAAATCGAGATAGTCGTGGGACAGCTTAATAGAc                                         >  2:445264/1‑148 (MQ=255)
                                                                                                            gCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActct                                      <  2:216580/151‑1 (MQ=255)
                                                                                                             ccGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACGCCCCCGCGCGCGACCAAACATCCCCGACACTGGCCCCCTCTGCACATCCAGATTTTGGTTGCTACGGTGCAAAACGAGATAGTCGTGGAACAGCTTAATAGACtctt                                     >  2:636295/1‑151 (MQ=255)
                                                                                                              cGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttc                                    <  1:162288/151‑1 (MQ=255)
                                                                                                              cGTTAAGCATACGCAGCGGCGGGGTCCCTTCGCCTTTTTTACGCCCCGGCGCGCGACCCAACAACAACGGCACCGGCACCCTCTGCACACCCCGAGTTTGGGTGCTACAGTGCGAATCCAGATAGTCGTGGAACAGCTTAATAGAc                                         >  1:409228/1‑146 (MQ=255)
                                                                                                                   aGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCCCCATCTGCACATCCAGATTTGGGGTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCttt                                 >  1:322193/1‑149 (MQ=255)
                                                                                                                     cATCCGCAGCGGCGGGTTCACTTCGCCTTCTTCCCGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGc                             <  1:40236/151‑1 (MQ=255)
                                                                                                                          gCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTa                        <  2:258837/151‑1 (MQ=255)
                                                                                                                             gcggcgGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATa                     <  2:524777/151‑1 (MQ=255)
                                                                                                                               ggcggGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAgg                   >  1:540913/1‑151 (MQ=255)
                                                                                                                                   ggTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGa                >  2:388888/1‑150 (MQ=255)
                                                                                                                                      tCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAacac            >  2:555214/1‑151 (MQ=255)
                                                                                                                                      tCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAaca             <  2:148151/150‑1 (MQ=255)
                                                                                                                                            cGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACAcgcg       <  1:523001/150‑1 (MQ=255)
                                                                                                                                             gCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACCGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGc     >  1:595203/1‑151 (MQ=255)
                                                                                                                                                tttttCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCccg  >  1:286418/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCG  >  NC_000913/4255912‑4256206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑