Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,382,351	C→T	intergenic (‑33/‑292)	frdA ← / → epmA	anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit/Elongation Factor P Lys34 lysyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,382,351	0	C	T	100.0%	212.9 / NA	57	intergenic (‑33/‑292)	frdA/epmA	anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit/Elongation Factor P Lys34 lysyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/31);  total (26/31)

TTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTAAATTATC  >  NC_000913/4382205‑4382495                                                                                                                                                   |                                                                                                                                                 ttttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGt                                                                                                                                              >  1:340714/1‑151 (MQ=255)   ttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGTGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGACTGTTTTTATCACACAGCTAAGTAc                                                                                                                                            >  1:87439/1‑151 (MQ=255)   ttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTa                                                                                                                                             <  2:635569/150‑1 (MQ=255)   ttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTAc                                                                                                                                            >  2:25527/1‑151 (MQ=255)             tGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCCGATTGTTTTTATCCCACCGCTACGTACTTCAGGGTa                                                                                                                                   >  1:488435/1‑150 (MQ=255)             tGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTa                                                                                                                                   >  2:317667/1‑150 (MQ=255)                   ttttGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTAcc                                                                                                                             <  1:415257/150‑1 (MQ=255)                     ttGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTg                                                                                                                           <  1:556400/150‑1 (MQ=255)                     tagCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTg                                                                                                                           >  2:156495/3‑150 (MQ=255)                      tGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGa                                                                                                                          >  1:412914/1‑150 (MQ=255)                       gCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGaa                                                                                                                         >  1:618123/1‑150 (MQ=255)                        cATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTg                                                                                                                           >  2:382872/1‑147 (MQ=255)                        cATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAg                                                                                                                       <  1:555732/151‑1 (MQ=255)                         aTTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGt                                                                                                                      <  1:83698/151‑1 (MQ=255)                          ttCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGtt                                                                                                                     >  2:610340/1‑151 (MQ=255)                           tCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGtt                                                                                                                     >  2:70188/1‑150 (MQ=255)                            cGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTa                                                                                                                    <  1:262460/150‑1 (MQ=255)                            cGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTAc                                                                                                                   <  2:453186/151‑1 (MQ=255)                             ggATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACg                                                                                                                  <  1:300331/151‑1 (MQ=255)                                   gCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGc                                                                                                            <  2:340725/151‑1 (MQ=255)                                       gcgcGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGaa                                                                                                         <  1:154887/150‑1 (MQ=255)                                       gcgcGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAAc                                                                                                        <  1:625182/151‑1 (MQ=255)                                         gcgGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGc                                                                                                      <  2:679421/151‑1 (MQ=255)                                           ggCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTa                                                                                                    <  2:613866/151‑1 (MQ=255)                                             cagcaATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGCTCGGCTTGAAAGGTCTGCCCGACATTCCTCCAGATTGTTTTTATCCCACAGCTACTTACTTCAGGGTAAGTACCTCAAAGTTACGGTCTGCGAATGCTAtt                                                                                                  >  1:344121/1‑151 (MQ=255)                                             cagcaATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTAtt                                                                                                  >  1:532948/1‑151 (MQ=255)                                                 aaTTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCAc                                                                                              >  1:248673/1‑151 (MQ=255)                                                                    gcgcCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTCTTCCACTGCTCCTTTATAGGTaca                                                                            >  1:624442/1‑150 (MQ=255)                                                                    gcgcCACCGGCGCATACAATGTCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTGCGTACTTCAGGGTAAGTGCCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGGaca                                                                            <  1:457468/150‑1 (MQ=255)                                                                      gcCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTacaaca                                                                         <  1:113253/151‑1 (MQ=255)                                                                        cACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGt                                                                       <  1:376427/151‑1 (MQ=255)                                                                         aCCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGt                                                                       >  2:271121/1‑150 (MQ=255)                                                                           cGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGGTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGtat                                                                     >  2:511567/1‑150 (MQ=255)                                                                            ggCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAg                                                                   <  1:573248/151‑1 (MQ=255)                                                                              cgcCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGt                                                                  >  1:217161/1‑150 (MQ=255)                                                                               gcCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCt                                                                <  1:181821/151‑1 (MQ=255)                                                                                   aCAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATg                                                            <  2:672550/151‑1 (MQ=255)                                                                                        ggCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATaa                                                       <  1:14652/151‑1 (MQ=255)                                                                                           aaGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTc                                                    >  2:108094/1‑151 (MQ=255)                                                                                            aGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCt                                                   <  1:3144/151‑1 (MQ=255)                                                                                                  gCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAAtt                                             <  1:383459/151‑1 (MQ=255)                                                                                                  gCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAAtt                                             >  1:42753/1‑151 (MQ=255)                                                                                                      gAATGGTTTGATCGGAATTCCTCCAGATTGTTTTTATCCCACAGCTGCGTTCGTCAGGGTAAGTGCCTGAAAGTTACGGTCTGCGAACGCTATGCCACTGCTCCTGTATAGGTACACCAGGATAGTCTGATGGATAAGTCTGAAATATGa                                          <  1:544846/150‑1 (MQ=255)                                                                                                        aaGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACga                                       <  1:528917/151‑1 (MQ=255)                                                                                                             ttGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACg                                        >  1:578868/1‑145 (MQ=255)                                                                                                             ttGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATc                                   <  1:60933/150‑1 (MQ=255)                                                                                                               gCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGa                                 <  1:72798/150‑1 (MQ=255)                                                                                                                 aCGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGAttt                              <  2:336091/151‑1 (MQ=255)                                                                                                                    aCATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGAtttttt                           >  2:156653/1‑151 (MQ=255)                                                                                                                     cATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGAttttttt                          >  2:437233/1‑151 (MQ=255)                                                                                                                         cctccAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTg                      <  1:228003/151‑1 (MQ=255)                                                                                                                          ctccAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTgc                     >  2:180499/1‑151 (MQ=255)                                                                                                                                   gTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAggg            <  2:621588/151‑1 (MQ=255)                                                                                                                                   gTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAggg            >  2:568523/1‑151 (MQ=255)                                                                                                                                   gTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAgg             <  1:465838/150‑1 (MQ=255)                                                                                                                                           cccACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTaaagt     <  1:564936/150‑3 (MQ=255)                                                                                                                                              acaGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTAAATTATc  >  1:667523/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                 TTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTAAATTATC  >  NC_000913/4382205‑4382495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑