Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,640,125	T→C	N61S (AAC→AGC)	arcA ←	response regulator in two‑component regulatory system with ArcB or CpxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,640,125	0	T	C	100.0%	177.8 / NA	47	N61S (AAC→AGC)	arcA	response regulator in two‑component regulatory system with ArcB or CpxA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/25);  total (22/25)

GTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTT  >  NC_000913/4639979‑4640265                                                                                                                                                   |                                                                                                                                             gTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCtt                                                                                                                                          >  2:630323/1‑151 (MQ=255)   tCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTa                                                                                                                                         <  2:561521/150‑1 (MQ=255)   tCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTa                                                                                                                                         <  2:432902/150‑1 (MQ=255)      cgcgGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCg                                                                                                                                     <  1:505454/151‑1 (MQ=255)             tGAACGGTTTGGCGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGAtt                                                                                                                              >  1:152521/1‑151 (MQ=255)               aaCGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTg                                                                                                                             >  1:395319/1‑150 (MQ=255)                      tGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCAt                                                                                                                     >  1:663188/1‑151 (MQ=255)                          gATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATc                                                                                                                 <  1:518835/151‑1 (MQ=255)                           aTGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCa                                                                                                                <  2:414099/151‑1 (MQ=255)                            tGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCa                                                                                                                <  1:109470/150‑1 (MQ=255)                                   aTCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTg                                                                                                        >  1:161638/1‑151 (MQ=255)                                       gCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTg                                                                                                        <  2:472567/147‑1 (MQ=255)                                       gCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGt                                                                                                    <  2:655162/151‑1 (MQ=255)                                        cACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTc                                                                                                   >  1:3116/1‑151 (MQ=255)                                          ccGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCat                                                                                                 >  2:117537/1‑151 (MQ=255)                                             aTTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATAtt                                                                                              <  1:146399/151‑1 (MQ=255)                                                  gAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCagag                                                                                         <  1:67405/151‑1 (MQ=255)                                                  gAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCagag                                                                                         <  2:462699/151‑1 (MQ=255)                                                       cGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGAt                                                                                    <  2:226132/151‑1 (MQ=255)                                                                     aCTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCcg                                                                      <  2:82280/151‑1 (MQ=255)                                                                       ttCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCcgcg                                                                    >  1:319842/1‑151 (MQ=255)                                                                             gTCACGGCCAGTCAGTAACATCAACGCGTTATGCGGCTGCTCGCGCAGTTCACGCGCTAAGAGAAGACCGCTATTGCCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCAATcg                                                              <  1:30205/151‑2 (MQ=255)                                                                               cACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGt                                                            <  2:108003/151‑1 (MQ=255)                                                                                     cAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTc                                                      >  2:122340/1‑151 (MQ=255)                                                                                      aGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCg                                                     <  1:246209/151‑1 (MQ=255)                                                                                          aGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGaaaa                                                 <  2:332309/151‑1 (MQ=255)                                                                                           ggAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAAc                                                >  2:660766/1‑151 (MQ=255)                                                                                             aaCATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAAcat                                              <  2:288232/151‑1 (MQ=255)                                                                                                aTCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAAcatcat                                           >  1:394843/1‑151 (MQ=255)                                                                                                       cAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTc                                    >  2:239108/1‑151 (MQ=255)                                                                                                        aaCATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTcc                                   >  2:256618/1‑151 (MQ=255)                                                                                                         aCATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCg                                  <  1:425134/151‑1 (MQ=255)                                                                                                              cGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCg                             >  2:383607/1‑151 (MQ=255)                                                                                                               gCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCg                             >  1:294381/1‑150 (MQ=255)                                                                                                                ccTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGaa                           <  1:412314/151‑1 (MQ=255)                                                                                                                   gCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAAt                        >  1:414381/1‑151 (MQ=255)                                                                                                                     tCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATAc                      >  1:683155/1‑151 (MQ=255)                                                                                                                         gcAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACtttt                  >  1:458682/1‑151 (MQ=255)                                                                                                                            gTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCaa               <  2:531374/151‑1 (MQ=255)                                                                                                                            gTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTGTCa                <  1:334962/150‑1 (MQ=255)                                                                                                                              tCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAAc              <  2:350588/150‑1 (MQ=255)                                                                                                                               cACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgt            <  2:37201/151‑1 (MQ=255)                                                                                                                                aCGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgtg           >  1:316732/1‑151 (MQ=255)                                                                                                                                       aaCAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtg    >  1:654703/1‑151 (MQ=255)                                                                                                                                       aaCAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgt     <  1:294269/150‑1 (MQ=255)                                                                                                                                         cAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGtt  >  2:676068/1‑151 (MQ=255)                                                                                                                                          agaagaCCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGtt  >  2:238050/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                             GTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTT  >  NC_000913/4639979‑4640265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑