Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	467,738	T→C	G109G (GGT→GGC)	cof →	thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	467,738	0	T	C	100.0%	161.8 / NA	44	G109G (GGT→GGC)	cof	thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/22);  total (22/22)

CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTT  >  NC_000913/467590‑467884                                                                                                                                                      |                                                                                                                                                   cGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACggctg                                                                                                                                                  <  1:329610/151‑1 (MQ=255)        gATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTAcc                                                                                                                                           <  1:281577/151‑1 (MQ=255)         aTTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCg                                                                                                                                          >  1:307122/1‑151 (MQ=255)           tACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCggg                                                                                                                                        >  1:603226/1‑151 (MQ=255)               ggCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAag                                                                                                                                    <  2:173856/151‑1 (MQ=255)                   aCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGAt                                                                                                                                <  1:329016/151‑1 (MQ=255)                            gcgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCgtt                                                                                                                       <  2:334603/151‑1 (MQ=255)                                gCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGttgttg                                                                                                                   <  2:465499/151‑1 (MQ=255)                                  aTTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGc                                                                                                                  >  2:133578/1‑150 (MQ=255)                                            aGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCTG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTc                                                                                                       >  2:360375/1‑151 (MQ=255)                                            aGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTc                                                                                                       <  1:467596/151‑1 (MQ=255)                                                 aaCTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAg                                                                                                  <  2:251517/151‑1 (MQ=255)                                                  aCTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGc                                                                                                 <  1:355945/151‑1 (MQ=255)                                                        aCATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGtttt                                                                                           <  1:104185/151‑1 (MQ=255)                                                           tCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGt                                                                                        >  2:118056/1‑151 (MQ=255)                                                            cGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGtt                                                                                       >  1:506984/1‑151 (MQ=255)                                                            cGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGtt                                                                                       >  1:195248/1‑151 (MQ=255)                                                            cGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGt                                                                                        <  1:442906/150‑1 (MQ=255)                                                               gatgatTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATc                                                                                    <  2:294093/151‑1 (MQ=255)                                                                     ttACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑CGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATa                                                                               >  1:311673/1‑150 (MQ=255)                                                                      tACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATaa                                                                              <  2:324709/150‑1 (MQ=255)                                                                         cTGCGGATGTCGCGGAGCTGGAGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCg                                                                           >  1:112353/1‑150 (MQ=255)                                                                           gCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGAt                                                                         <  2:640614/150‑1 (MQ=255)                                                                                   cgcgGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCaaaaa                                                                 >  2:330108/1‑150 (MQ=255)                                                                                            ggTGCTGTATCAGCAATGGGGTACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCAc                                                         <  1:306239/149‑1 (MQ=255)                                                                                              tGCTGTATCAGCCATGGGAAACCCGCAGCC‑GGATGGATAACTTCAATCACGACCGCTGGTTTACCGGGAAAGAGATCCCTGCCTTGTTGCAGGCATTTGTTTATAGCCGTTTTCGGTATCCGATAATCTATGGCAAAAAAATGCCACTc                                                       <  1:33540/149‑1 (MQ=255)                                                                                               gCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCgg                                                     <  2:104453/150‑1 (MQ=255)                                                                                                 tGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGc                                                    >  1:372539/1‑149 (MQ=255)                                                                                                  gTATCAGCAATGGGATCCCCG‑AGCCAGAATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATCATCGCTGTCAAAAAAATGCCCCTCGGCa                                                   <  1:71017/149‑1 (MQ=255)                                                                                                      cAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTc                                              >  1:239731/1‑150 (MQ=255)                                                                                                       aGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCAc                                            <  2:511814/151‑1 (MQ=255)                                                                                                           aTGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCa                                          >  2:596511/1‑149 (MQ=255)                                                                                                               gATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCt                                    <  2:466734/151‑1 (MQ=255)                                                                                                                aTACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGGTGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCt                                    >  1:447726/1‑150 (MQ=255)                                                                                                                aTACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTg                                   >  2:487707/1‑151 (MQ=255)                                                                                                                 tACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGc                                  >  1:67330/1‑151 (MQ=255)                                                                                                                   cccG‑AGCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCtt                                >  1:62533/1‑151 (MQ=255)                                                                                                                         gCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGGTGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtgtg                           <  1:581906/151‑1 (MQ=255)                                                                                                                         gCCAGCATGCATATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtgt                            >  1:647443/1‑150 (MQ=255)                                                                                                                               atgcatATCTTCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATc                     >  1:554359/1‑151 (MQ=255)                                                                                                                                         tCAATGACGACGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCAcgacga              <  1:598199/148‑1 (MQ=255)                                                                                                                                              gacgacGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTacac      >  1:444267/1‑151 (MQ=255)                                                                                                                                              gacgacGGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTacac      <  2:238136/151‑1 (MQ=255)                                                                                                                                                 gacgGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCt   >  1:386995/1‑151 (MQ=255)                                                                                                                                                  acgGCTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCtt  >  1:542803/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                   CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCG‑AGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTT  >  NC_000913/467590‑467884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑