Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	599,828	T→C	L372P (CTG→CCG)	cusA →	copper/silver efflux system, membrane component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	599,828	0	T	C	100.0%	147.4 / NA	43	L372P (CTG→CCG)	cusA	copper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

ACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCGAGAATGCGCATAAACGGCTGGAAGAGTG  >  NC_000913/599680‑599975                                                                                                                                                     |                                                                                                                                                    acaacaTACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCgg                                                                                                                                                   >  1:333259/1‑151 (MQ=255)        aCGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTtgt                                                                                                                                             <  2:668578/150‑1 (MQ=255)         cGATCGCAGCCAGCTCATTGACCGCGCCATCGCCAACCTCAGCGGCAAGTTGCTGGAAGAGATTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGtt                                                                                                                                               >  1:636628/1‑147 (MQ=255)         cGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTtgt                                                                                                                                             >  1:197284/1‑149 (MQ=255)          gATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTa                                                                                                                                          <  1:178254/151‑1 (MQ=255)           aTCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGCGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTAt                                                                                                                                         >  1:364115/1‑151 (MQ=255)            acGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGGAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGGGGCGATTATTTCGTTGCCGCCGGGGGTGTGGAtt                                                                                                                                        >  1:252876/2‑151 (MQ=255)             cGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTg                                                                                                                                       >  2:405081/1‑151 (MQ=255)               cagccagcTCATTGACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCt                                                                                                                                     <  2:78670/151‑1 (MQ=255)                     gcTCATTGACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTAtt                                                                                                                               <  2:395412/151‑1 (MQ=255)                          ttGACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCAt                                                                                                                          >  2:599248/1‑151 (MQ=255)                                  gcCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTcc                                                                                                                  >  2:296698/1‑151 (MQ=255)                                   ccATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATTTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCGCTTCCa                                                                                                                 <  1:617219/151‑1 (MQ=255)                                       cGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAggg                                                                                                              <  2:243282/150‑1 (MQ=255)                                          cAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTg                                                                                                          <  2:160002/151‑1 (MQ=255)                                           aaCCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGGAGTCTGTGCGCTGTTTCCCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCCGGGACTg                                                                                                          >  1:484868/1‑150 (MQ=255)                                             ccTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAAt                                                                                                       >  1:656772/1‑151 (MQ=255)                                                cAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATg                                                                                                      <  2:683024/149‑1 (MQ=255)                                                    ggCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAata                                                                                                <  1:66540/151‑1 (MQ=255)                                                        aGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTa                                                                                                                                 <  1:130096/114‑1 (MQ=255)                                                        aGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAAtattat                                                                                            >  1:128238/1‑151 (MQ=255)                                                        aGTTGCTGGAAGAGTTTATTGTGGGGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTa                                                                                                                                 >  2:130099/1‑114 (MQ=255)                                                               ggAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGGATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGt                                                                                       >  2:309517/1‑149 (MQ=255)                                                                   gagTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTg                                                                                 <  1:579476/151‑1 (MQ=255)                                                                         aTTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTg                                                                           <  2:255549/151‑1 (MQ=255)                                                                           tgtgGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGc                                                                          <  1:401509/150‑1 (MQ=255)                                                                              ggtggCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGAt                                                                       <  1:176530/150‑1 (MQ=255)                                                                               gtggCGGTAGTCTGTGCGCTGTTTTTCTGGGATGTGCGCCCGGCGCTCGGGGCGGGTATTTTGTTGCCGCCGGGGGTGTGTATTGCTTTTATTTTCATTCACTTCCAGGGGCTGGATGCCCATATTATGTCGTTGGGTGGCAttttccgt                                                                      >  1:572122/1‑144 (MQ=255)                                                                                       aGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCgg                                                              <  2:269481/150‑1 (MQ=255)                                                                                            gtgCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCg                                                          <  1:405068/149‑1 (MQ=255)                                                                                              gcgcTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATg                                                       <  1:275465/150‑1 (MQ=255)                                                                                                 cTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCg                                                   >  2:529509/1‑151 (MQ=255)                                                                                                                      tCGGCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCg                              >  2:310549/1‑151 (MQ=255)                                                                                                                        ggCGCTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCgag                            <  2:512038/151‑1 (MQ=255)                                                                                                                         gcgcTGGTGGCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCg                              >  2:219246/1‑148 (MQ=255)                                                                                                                              ggtggCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCGAGAATg                        <  2:436225/149‑1 (MQ=255)                                                                                                                                tggCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCGAGAATgcgc                     >  2:129476/1‑150 (MQ=255)                                                                                                                                 ggCGATTATTTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCGAGAATGCGCAt                   <  1:354037/151‑1 (MQ=255)                                                                                                                                        attTCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCGAGAATGCGCATAAACgg             <  1:12654/150‑1 (MQ=255)                                                                                                                                          ttCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCGAGAATGCGCATAAACGGCt           <  1:547548/150‑1 (MQ=255)                                                                                                                                          ttCGTTGCCGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCGAGAATGCGCATAAACGGCt           <  2:619431/150‑1 (MQ=255)                                                                                                                                                  cGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCGAGAATGCGCATAAACGGCTGGAAGAGTg  >  2:606000/1‑151 (MQ=255)                                                                                                                                                  cGCCGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCGAGAATGCGCATAAACGGCTGGAAGAGTg  >  1:551049/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    ACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTGGGGTTGTGTATTGCTTTTATTGTCATGCACTTCCAGGGACTGAATGCCAATATTATGTCGTTGGGTGGCATTGCGATTGCCGTCGGGGCGATGGTCGATGCTGCTATCGTCATGATCGAGAATGCGCATAAACGGCTGGAAGAGTG  >  NC_000913/599680‑599975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑