Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	602,007	C→T	P17S (CCG→TCG)	pheP →	phenylalanine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	602,007	0	C	T	97.6%	155.0 / NA	41	P17S (CCG→TCG)	pheP	phenylalanine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (17/23);  minor base G (0/1);  total (17/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGG  >  NC_000913/601867‑602150                                                                                                                                             |                                                                                                                                                aCCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCa                                                                                                                                       <  2:472548/151‑1 (MQ=255)   cccGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATc                                                                                                                                     <  2:523919/151‑1 (MQ=255)         tAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGAt                                                                                                                               >  2:344181/1‑151 (MQ=255)           aCCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTa                                                                                                                             <  2:447027/151‑1 (MQ=255)                gtgGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATaa                                                                                                                        <  1:666661/151‑1 (MQ=255)                gtgGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATa                                                                                                                         >  2:21117/1‑150 (MQ=255)                gtgGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATa                                                                                                                         <  2:592979/150‑1 (MQ=255)                gtgGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATa                                                                                                                         >  1:477339/1‑150 (MQ=255)                  ggATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAAcc                                                                                                                      <  2:282707/151‑1 (MQ=255)                   gATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCg                                                                                                                     >  1:34735/1‑151 (MQ=255)                            ttGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTc                                                                                                             >  2:189046/1‑150 (MQ=255)                             tGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACTGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCa                                                                                                            <  1:418775/150‑1 (MQ=255)                             tGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCa                                                                                                            <  1:158944/150‑1 (MQ=255)                             tGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCa                                                                                                            <  2:173328/150‑1 (MQ=255)                                  cgaTGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGGATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGAt                                                                                                      >  2:688288/1‑151 (MQ=255)                                    aTGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTg                                                                                                    <  2:678843/151‑1 (MQ=255)                                       ggCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGt                                                                                                 <  1:334814/151‑1 (MQ=255)                                          aCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTgg                                                                                              >  1:365454/1‑151 (MQ=255)                                          aCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTgg                                                                                              <  2:298450/151‑1 (MQ=255)                                          aCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTg                                                                                               <  2:573755/150‑1 (MQ=255)                                             aaaTGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTg                                                                                           <  1:330855/151‑1 (MQ=255)                                                   tAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCa                                                                                      <  2:477360/150‑1 (MQ=255)                                                    aaaaGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAAt                                                                                    <  2:250178/151‑1 (MQ=255)                                                         gTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGt                                                                                >  1:553722/1‑150 (MQ=255)                                                         cggCCCCTCAAAAAACAAGTCTCACAGGGGAAAGGCGGGAGAAACGCGGCAACAGGATCGTTAGATACTACGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGACATATTCAACTGATTGCGTTGGGCGGCGCaa                                                                                     >  1:400995/3‑145 (MQ=255)                                                            ccccTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTg                                                                            <  1:596194/151‑1 (MQ=255)                                                                              acacaGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTg                                                          >  2:381538/1‑151 (MQ=255)                                                                                 caGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGcc                                                       <  2:369994/151‑1 (MQ=255)                                                                                       aaaGGCGGGAAAAACGCATCAACCGTATCGGAAGATACTGCGGCGAATCAAGAGGCGGCGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCg                                                  <  1:84479/150‑1 (MQ=255)                                                                                         aGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGACTGTTTCTTGGCATTGGCCCGGCGAtt                                               >  1:384799/1‑151 (MQ=255)                                                                                            cGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAg                                            <  1:406086/151‑1 (MQ=255)                                                                                                        gTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCg                                <  1:240559/151‑1 (MQ=255)                                                                                                           aaCCGTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCt                             <  1:255350/151‑1 (MQ=255)                                                                                                               gTATCGGAAGATACTGCGTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTAt                         >  1:471178/1‑151 (MQ=255)                                                                                                                                gTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTc        >  1:132354/1‑151 (MQ=255)                                                                                                                                gTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTc        <  2:224239/151‑1 (MQ=255)                                                                                                                                gTCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTc        <  1:378652/151‑1 (MQ=255)                                                                                                                                 tCGAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCg       >  1:370121/1‑151 (MQ=255)                                                                                                                                   gAATCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGGCCGGCTGTATTGCTGGGCTACGGCGTCGcc     >  1:661929/1‑151 (MQ=255)                                                                                                                                     aTCAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCgg   >  1:552909/1‑151 (MQ=255)                                                                                                                                       cAAGAGTCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCGGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCggg  >  1:110884/1‑150 (MQ=255)                                                                                                                                             |                                                                                                                                                ACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGG  >  NC_000913/601867‑602150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑