Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	657,830	T→C	T37A (ACG→GCG)	flc ←	fluoride efflux channel, dual topology membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	657,830	0	T	C	100.0%	92.1 / NA	26	T37A (ACG→GCG)	flc	fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/12);  total (14/12)

GCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTG  >  NC_000913/657685‑657970                                                                                                                                                  |                                                                                                                                             gCCGAAAATGTTGAGAGGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCcccc                                                                                                                                          <  1:684007/150‑1 (MQ=255)                   gttgttAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGc                                                                                                                        >  1:612041/1‑150 (MQ=255)                     tgttAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGt                                                                                                                     >  2:490879/1‑151 (MQ=255)                       ttAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAg                                                                                                                      <  1:453428/148‑1 (MQ=255)                        tAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTggg                                                                                                                  >  2:489400/1‑151 (MQ=255)                         acaCCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGACTCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCa                                                                                                                       <  1:113695/143‑1 (MQ=255)                              gCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAAt                                                                                                            <  1:606850/151‑1 (MQ=255)                                        ccGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACtt                                                                                                   <  2:676580/150‑1 (MQ=255)                                             ggtgATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAg                                                                                              <  2:433118/150‑1 (MQ=255)                                                            ccACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTGTGCTGTCAGCTCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCgcgc                                                                                <  1:639369/149‑1 (MQ=255)                                                                          tGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGcc                                                                >  1:608616/1‑151 (MQ=255)                                                                          tGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGcc                                                                >  2:221521/1‑151 (MQ=255)                                                                                   tCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAAc                                                       >  1:263590/1‑151 (MQ=255)                                                                                          gAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTaa                                                 >  1:19873/1‑150 (MQ=255)                                                                                           aaCCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTaaa                                                >  1:237050/1‑150 (MQ=255)                                                                                             ccATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTaaa                                                >  2:163462/1‑148 (MQ=255)                                                                                               aTGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAaaga                                             >  1:182050/1‑149 (MQ=255)                                                                                                 gCGAATCCTATCCCTATGATGAATGCCCAAATCAGGTTTGCTGTCAGCCCCCCCAACGGAATCGCCTGATGCAGTGGTTTAAATCGCATACTTAACAGCCATCTCGCCACGCTCCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGtt                                         <  2:411205/151‑1 (MQ=255)                                                                                                   gAATCTTATTCCTATGATGAATGCCCAAAGCAGGTTTGCTGTAAGCCCCCCCAACGGATCCGCCTGATGCAGTGGTTTAAACCGCATAATAAACACCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAaagaa                                            <  1:472370/146‑1 (MQ=255)                                                                                                       cctattcctatGATGAATGCCCCAATCGGGTTTGCTGTCAGCGCCCCCAACGAAATCGCCTGATGCAGTGGGTTAAATCGCATCCTAAACACCCATCTCGCCCCGCTTCCCTTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAac                                    <  2:560940/150‑1 (MQ=255)                                                                                                           ttcctatGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGc                               >  1:519189/1‑151 (MQ=255)                                                                                                             cctatGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCa                              >  1:82250/1‑150 (MQ=255)                                                                                                                            ccAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTatttg               >  1:49787/1‑150 (MQ=255)                                                                                                                            ccAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTatttg               >  2:347810/1‑150 (MQ=255)                                                                                                                                        gCTGTCAGCCCCCCAAACGGAATCGCCTGATGCAGGGGGTTAAACCTCATACTTAACAACCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAg    <  1:174722/149‑1 (MQ=255)                                                                                                                                          tGTCAGCCCCCCAAGCGGAATCGCGTGATGCAGTGGTTTAAATCGCATATTTAACAGCCACCCCCCCGCGTTCCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTg  <  2:603396/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                             GCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTG  >  NC_000913/657685‑657970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑