Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	871,451	T→C	L162P (CTG→CCG)	gsiC →	glutathione ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	871,451	0	T	C	100.0%	94.0 / NA	26	L162P (CTG→CCG)	gsiC	glutathione ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/12);  total (14/12)

GGCGGCGGGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCTGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTAAGCGAAGATTATATGCGTACCGCGAGGGCGA  >  NC_000913/871308‑871597                                                                                                                                                |                                                                                                                                                   ggcggcGGGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTAcc                                                                                                                                             <  2:436486/151‑1 (MQ=255) ggcggcGGGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTAcc                                                                                                                                             <  1:96760/151‑1 (MQ=255)   cggcggGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGt                                                                                                                                           <  1:281354/151‑1 (MQ=255)      cggGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTggg                                                                                                                                        >  1:641811/1‑151 (MQ=255)                cgccgcCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGt                                                                                                                              >  2:315632/1‑151 (MQ=255)                            gCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTAc                                                                                                                  >  2:11789/1‑151 (MQ=255)                                 aCCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACAtttt                                                                                                             <  1:172293/151‑1 (MQ=255)                                 aCCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACAtttt                                                                                                             <  1:261695/151‑1 (MQ=255)                                   cGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTAc                                                                                                           <  2:74622/151‑1 (MQ=255)                                          cGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTAccctccct                                                                                                    >  1:257261/1‑151 (MQ=255)                                           ggATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTg                                                                                                   <  1:570916/151‑1 (MQ=255)                                               cGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACg                                                                                                <  1:345267/150‑1 (MQ=255)                                                         tGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGc                                                                                     >  2:475005/1‑151 (MQ=255)                                                              aTTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTgg                                                                                >  1:191002/1‑151 (MQ=255)                                                                  cGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGgccgc                                                                            >  1:196462/1‑151 (MQ=255)                                                                                      ccGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTAccc                                                        <  2:252349/151‑1 (MQ=255)                                                                                      ccGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTAccc                                                        >  1:438483/1‑151 (MQ=255)                                                                                               gCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGcgtcgt                                               <  2:454376/151‑1 (MQ=255)                                                                                                      ggATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCg                                         >  2:30084/1‑150 (MQ=255)                                                                                                           cTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGttt                                   >  1:41837/1‑151 (MQ=255)                                                                                                             tttAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGtttt                                  >  2:681308/1‑150 (MQ=255)                                                                                                               tAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTaa                                <  2:124604/150‑1 (MQ=255)                                                                                                               tAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTaa                                <  1:76540/150‑1 (MQ=255)                                                                                                                     aGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTAAGCGa                            >  1:512726/1‑148 (MQ=255)                                                                                                                       gTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCACTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTAAGCGAAGAt                        >  1:54014/1‑150 (MQ=255)                                                                                                                           tctcCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTAAGCGAAGATTatat                   >  1:228175/1‑151 (MQ=255)                                                                                                                                             ggccgCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTAAGCGAAGATTATATGCGTACCGCGAGGGCGa  <  2:477383/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   GGCGGCGGGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCTGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTAAGCGAAGATTATATGCGTACCGCGAGGGCGA  >  NC_000913/871308‑871597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑