Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,076,582	A→G	R767R (CGT→CGC)	putA ←	fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,076,582	0	A	G	100.0%	141.1 / NA	38	R767R (CGT→CGC)	putA	fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/17);  total (21/17)

GCGATCCCTTGCGCGGCAATCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTT  >  NC_000913/1076436‑1076729                                                                                                                                                   |                                                                                                                                                    gCGATCCCTTGCGCGGCAATCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCgg                                                                                                                                                  >  1:584062/1‑150 (MQ=255)    aTCCCTTGCGCGGCAATCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTggg                                                                                                                                              >  1:296317/1‑151 (MQ=255)          tGCGCGGCAATCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTc                                                                                                                                          <  2:318232/149‑1 (MQ=255)            cgcgGCAATCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGt                                                                                                                                        >  1:648407/1‑149 (MQ=255)                  aaTCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGaa                                                                                                                                 >  2:281903/1‑150 (MQ=255)                   aTCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAat                                                                                                                               >  2:207594/1‑151 (MQ=255)                     cAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAatca                                                                                                                             <  1:266442/151‑1 (MQ=255)                     cAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAatca                                                                                                                             <  1:281899/151‑1 (MQ=255)                                  tCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTc                                                                                                                >  2:203123/1‑151 (MQ=255)                                     gCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTcc                                                                                                               <  1:218264/149‑1 (MQ=255)                                     gCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCg                                                                                                              <  1:467716/150‑1 (MQ=255)                                     gCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCg                                                                                                              <  2:404812/150‑1 (MQ=255)                                         gTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCGCAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCgta                                                                                                         <  1:410471/151‑1 (MQ=255)                                                cAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTgg                                                                                                  >  2:297592/1‑151 (MQ=255)                                                         gTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATc                                                                                          <  2:468907/150‑1 (MQ=255)                                                           tACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGa                                                                                        <  2:27441/150‑1 (MQ=255)                                                             ccTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGTGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTCAGCGAAATCATCCCGCACCTGTCCGGCGTCGTAGTGGAGAAAATCGTcc                                                                                      >  1:471545/1‑150 (MQ=255)                                                             ccTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCg                                                                                     <  2:170651/151‑1 (MQ=255)                                                               tGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCt                                                                                   >  2:444111/1‑151 (MQ=255)                                                                   gccAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTcgc                                                                               >  1:420570/1‑151 (MQ=255)                                                                      aGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCgcgc                                                                             >  2:603136/1‑150 (MQ=255)                                                                        tGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACt                                                                          >  1:138407/1‑151 (MQ=255)                                                                                  aTCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATgg                                                                >  1:570981/1‑151 (MQ=255)                                                                                       cccGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGtt                                                            >  1:285943/1‑150 (MQ=255)                                                                                          ggTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTg                                                        >  2:516970/1‑151 (MQ=255)                                                                                            tGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGa                                                       >  1:390106/1‑150 (MQ=255)                                                                                               aaaTAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGt                                                   <  2:625433/151‑1 (MQ=255)                                                                                               aaaTAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGt                                                   <  1:648591/151‑1 (MQ=255)                                                                                                aaTAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGtt                                                  <  2:148363/151‑1 (MQ=255)                                                                                                 aTAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGttt                                                 >  2:289066/1‑151 (MQ=255)                                                                                                      cAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCg                                            <  1:230209/151‑1 (MQ=255)                                                                                                            gAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCa                                      <  1:386732/151‑1 (MQ=255)                                                                                                                 tCCACGGACTGATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCa                                  <  2:531908/150‑1 (MQ=255)                                                                                                                   cACGGACTGATACACACCACAGTCCCTAATGGGAGGTGGGTTTCGTTAGCGAAATCATCCAGCACCCGTCCGGCGTAGTAGCGGAAAAAATCGACCGCTTCGCGCACTTCGCCAATAGAGTTACTGAAGGTTTTTTCGGCCTCATCCAcc                                >  1:664324/1‑150 (MQ=255)                                                                                                                           gATACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCa                       <  2:154166/151‑1 (MQ=255)                                                                                                                            aTACACACCACAGGCCCTAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCa                       >  2:431811/1‑150 (MQ=255)                                                                                                                                             tAATGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTgg     >  2:11852/1‑151 (MQ=255)                                                                                                                                                tGGGCGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCtt  >  1:341766/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GCGATCCCTTGCGCGGCAATCAGCGGCGTTTGTTCTGCCGGTTTTGCCAGCACGCTGTTACCTGCCGCCAGTGCGGCGGCGATCTGCCCGGTGAAAATAGCCAGCGGGAAGTTCCACGGACTGATACACACCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTT  >  NC_000913/1076436‑1076729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑