Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,084,191	A→G	V272V (GTA→GTG)	efeB →	deferrrochelatase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,084,191	0	A	G	100.0%	169.7 / NA	46	V272V (GTA→GTG)	efeB	deferrrochelatase, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/17);  total (29/17)

GCAAAGAGACGCCGATTAATTTGCTGGGTTTCAAAGACGGCACTGCCAATCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTACGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTGATTACGCCAGCGACCCGGAAG  >  NC_000913/1084043‑1084336                                                                                                                                                     |                                                                                                                                                  gCAAAGAGACGCCGATTAATTTGCTGGGTTTCAAAGACGGCACTGCCAATCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCg                                                                                                                                                 >  1:142296/1‑151 (MQ=255)   caaGAGACGCCGATTAATTTGCTGGGTTTCAAAGACGGCACTGCCAATCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCg                                                                                                                                                 >  1:626309/2‑149 (MQ=255)   aaaGAGACGCCGATTAATTTGCTGGGTTTCAAAGACGGCACTGCCAATCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCt                                                                                                                                               >  1:129656/1‑151 (MQ=255)      gagaCGCCGATTAATTTGCTGGGTTTCAAAGACGGCACTGCCAATCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGa                                                                                                                                            <  2:238546/151‑1 (MQ=255)          cGCCGATTAATTTGCTGGGTTTCAAAGACGGCACTGCCAATCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTc                                                                                                                                         >  2:452101/1‑150 (MQ=255)          cGCCGATTAATTTGCTGGGTTTCAAAGACGGCACTGCCAATCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCa                                                                                                                                        <  1:123923/151‑1 (MQ=255)               aTTAATTTGCTGGGTTTCAAAGACGGCACTGCCAATCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGttt                                                                                                                                    <  2:255644/150‑1 (MQ=255)                ttAATTTGCTGGGTTTCAAAGACGGCACTGCCAATCCCGATAGCCAGACTGATAAGTTTATGCAAACAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCg                                                                                                                                  >  1:79677/1‑151 (MQ=255)                  aaTTTGCTGGGCTTCAAAGACGGCACTGCCAATCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCg                                                                                                                                  >  1:569571/1‑149 (MQ=255)                              ttCAAAGACGGCACTGCCAATCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTggg                                                                                                                    >  1:686012/1‑151 (MQ=255)                                 aaaGACGGCACTGCCAATCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACa                                                                                                                 <  2:264912/151‑1 (MQ=255)                                                  tCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGa                                                                                                 >  1:413221/1‑150 (MQ=255)                                                      gATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAAcagc                                                                                            >  1:148520/1‑151 (MQ=255)                                                      gATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAAcagc                                                                                            >  2:218545/1‑151 (MQ=255)                                                       aTAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAAcagca                                                                                           <  2:116293/151‑1 (MQ=255)                                                       aTAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAAcagc                                                                                            >  2:442045/1‑150 (MQ=255)                                                       aTAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAAcagc                                                                                            >  1:523369/1‑150 (MQ=255)                                                            cAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACg                                                                                       >  2:609506/1‑150 (MQ=255)                                                             aGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGAt                                                                                     <  2:111164/151‑1 (MQ=255)                                                              gAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCTGCTGAAAGAACAGCAGACGAtt                                                                                    >  1:595711/1‑151 (MQ=255)                                                              gAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGAtt                                                                                    >  1:285052/1‑151 (MQ=255)                                                              gAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGAtt                                                                                    <  2:413234/151‑1 (MQ=255)                                                              gAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGAtt                                                                                    <  2:348911/151‑1 (MQ=255)                                                               aaTGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGCttt                                                                                   >  1:531596/1‑151 (MQ=255)                                                                    tAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGc                                                                              >  1:423023/1‑151 (MQ=255)                                                                      aGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCg                                                                            >  2:267408/1‑151 (MQ=255)                                                                        ttGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTg                                                                          >  1:317803/1‑151 (MQ=255)                                                                           aTGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATa                                                                       <  2:595740/151‑1 (MQ=255)                                                                            tGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATa                                                                       >  2:112099/1‑150 (MQ=255)                                                                              cAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAg                                                                     >  1:133981/1‑150 (MQ=255)                                                                              cAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGc                                                                    <  1:150633/151‑1 (MQ=255)                                                                                  aaGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAAc                                                                >  2:570012/1‑151 (MQ=255)                                                                                                    cAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAAt                                              <  2:296114/151‑1 (MQ=255)                                                                                                     aGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATg                                             >  2:468219/1‑151 (MQ=255)                                                                                                     aGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATg                                             >  2:466443/1‑151 (MQ=255)                                                                                                      gATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATgc                                            <  2:425386/151‑1 (MQ=255)                                                                                                       aTCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATgc                                            >  1:447452/1‑150 (MQ=255)                                                                                                       aTCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATgc                                            >  1:471585/1‑150 (MQ=255)                                                                                                        tCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATgc                                            >  1:662797/1‑149 (MQ=255)                                                                                                                          gACAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCt                        <  2:356916/151‑1 (MQ=255)                                                                                                                           aCAATCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTg                       <  2:57260/151‑1 (MQ=255)                                                                                                                              aTCGGTGGCAGCTATCAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTGAtt                    <  2:458069/151‑1 (MQ=255)                                                                                                                                cGGTGGCAGCTATCTGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGACCGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACAGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTGATTAc                  >  1:251543/1‑151 (MQ=255)                                                                                                                                  gTGGCAGCTATAAGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGAAGACGATTTTTGGCCGGGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTGATTACg                 >  1:192449/1‑150 (MQ=255)                                                                                                                                              aGGCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTGATTACGCCAGCGACCCgga    <  2:129658/151‑1 (MQ=255)                                                                                                                                                gCAGTGCGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTGATTACGCCAGCGACCCggaag  <  2:344411/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GCAAAGAGACGCCGATTAATTTGCTGGGTTTCAAAGACGGCACTGCCAATCCCGATAGCCAGAATGATAAGTTGATGCAAAAAGTGGTGTGGGTAACGGCAGATCAGCAGGAGCCTGCGTGGACAATCGGTGGCAGCTATCAGGCAGTACGCTTGATTCAGTTTCGAGTGGAATTTTGGGACAGAACGCCGCTGAAAGAACAGCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTGATTACGCCAGCGACCCGGAAG  >  NC_000913/1084043‑1084336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑