Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,838	T→C	G179G (GGA→GGG)	ldtC ←	L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,838	0	T	C	100.0%	130.9 / NA	37	G179G (GGA→GGG)	ldtC	L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/21);  total (16/21)

GATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACC  >  NC_000913/1169700‑1169984                                                                                                                                           |                                                                                                                                                   gATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCAc                                                                                                                                         >  1:606640/1‑150 (MQ=255) gATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACt                                                                                                                                        <  2:117400/151‑1 (MQ=255)    atCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTAcc                                                                                                                                     >  1:586332/1‑151 (MQ=255)       gTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGACGCAAATAAACGCCGCCATACCCCGCCAGACGACTCGCATGCTGGCCCATTGGGCCATCCAGCCCAGCCGGCACTAcc                                                                                                                                     >  1:147184/1‑148 (MQ=255)          aTCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGc                                                                                                                               >  2:42043/1‑151 (MQ=255)                  gACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAAt                                                                                                                       <  1:171609/151‑1 (MQ=255)                  gACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAAt                                                                                                                       >  1:452373/1‑151 (MQ=255)                      aaTACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTccc                                                                                                                   >  1:525896/1‑151 (MQ=255)                       aTACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCt                                                                                                                  <  1:613918/151‑1 (MQ=255)                                     tACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAAc                                                                                                      <  2:85893/149‑1 (MQ=255)                                      aCCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACggg                                                                                                   >  2:386201/1‑151 (MQ=255)                                            aTGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCgg                                                                                              >  2:208767/1‑150 (MQ=255)                                                  aTGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTg                                                                                       <  1:407867/151‑1 (MQ=255)                                                        aaaTCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTg                                                                                 >  2:440238/1‑151 (MQ=255)                                                         aaTCGGCGTTCGTACCATGAAGCAAATAATCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGtt                                                                                  <  1:653203/149‑1 (MQ=255)                                                            cGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGc                                                                               <  2:520233/149‑1 (MQ=255)                                                            cGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCg                                                                              <  2:153854/150‑1 (MQ=255)                                                              gCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTcc                                                                           <  1:629042/151‑1 (MQ=255)                                                                  tCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGt                                                                       >  2:562845/1‑151 (MQ=255)                                                                  tCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGt                                                                       >  2:272364/1‑151 (MQ=255)                                                                    gTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTg                                                                     <  2:338552/151‑1 (MQ=255)                                                                      aCCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTggg                                                                   >  2:453554/1‑151 (MQ=255)                                                                            aaGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGc                                                              >  2:178487/1‑150 (MQ=255)                                                                                aaaTAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGtt                                                         <  2:349776/151‑1 (MQ=255)                                                                                     aaCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCt                                                    <  2:124374/151‑1 (MQ=255)                                                                                     aaCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCt                                                    >  1:680827/1‑151 (MQ=255)                                                                                     aaCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCt                                                    <  1:594510/151‑1 (MQ=255)                                                                                        gccgccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCt                                                    <  2:625685/148‑1 (MQ=255)                                                                                        gccgccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCt                                                    >  1:625654/1‑148 (MQ=255)                                                                                         ccgccATAGGCAGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGaa                                                 <  1:438979/150‑1 (MQ=255)                                                                                           gccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACg                                              <  2:84507/151‑1 (MQ=255)                                                                                            ccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACgg                                             >  1:478528/1‑151 (MQ=255)                                                                                                  gccgccAGACGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCa                                       <  2:573795/151‑1 (MQ=255)                                                                                                           cGAATCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCg                              <  2:332118/151‑1 (MQ=255)                                                                                                             aaTCGCATGATGGCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGgt                            <  2:430780/151‑1 (MQ=255)                                                                                                                        ggCCCATTGGGTTATCCAGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCgtgt                  <  2:453539/150‑1 (MQ=255)                                                                                                                         gctcATTTGGTTATCCAGCCGAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCgtgt                  <  1:119837/146‑1 (MQ=255)                                                                                                                                       ccagcccagcCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGAcc  >  2:155115/1‑151 (MQ=255)                                                                                                                                           |                                                                                                                                                   GATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACC  >  NC_000913/1169700‑1169984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑