Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,216,705	A→G	intergenic (+70/‑43)	ariR → / → ymgC	RcsB connector protein for regulation of biofilm and acid‑resistance/Blue light, low temperature and stress induced protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,216,705	0	A	G	100.0%	183.7 / NA	46	intergenic (+70/‑43)	ariR/ymgC	RcsB connector protein for regulation of biofilm and acid‑resistance/Blue light, low temperature and stress induced protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/24);  total (22/24)

ACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATT  >  NC_000913/1216558‑1216852                                                                                                                                                    |                                                                                                                                                    acaaGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATgcac                                                                                                                                                  >  1:4546/1‑151 (MQ=255)    aGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGc                                                                                                                                               >  2:408353/1‑151 (MQ=255)     gTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGc                                                                                                                                               >  2:16922/1‑150 (MQ=255)          atTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAAt                                                                                                                                          <  1:442352/150‑1 (MQ=255)                   gCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGt                                                                                                                                >  2:337150/1‑151 (MQ=255)                     tGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGtt                                                                                                                               >  2:536051/1‑150 (MQ=255)                      gATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTa                                                                                                                              <  2:136970/150‑1 (MQ=255)                               aGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTAc                                                                                                                    <  1:21479/151‑1 (MQ=255)                                  aaaaCACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAg                                                                                                                 <  1:501743/151‑1 (MQ=255)                                  aaaaCACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAg                                                                                                                 <  2:316322/151‑1 (MQ=255)                                      cacTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTg                                                                                                             <  2:103180/151‑1 (MQ=255)                                       acTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTg                                                                                                             <  1:55064/150‑1 (MQ=255)                                                 gtgtTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGaataat                                                                                                  >  2:463554/1‑151 (MQ=255)                                                     tGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCa                                                                                               >  2:462379/1‑150 (MQ=255)                                                     tGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCa                                                                                               >  1:531837/1‑150 (MQ=255)                                                           acacAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCag                                                                                        <  2:420830/151‑1 (MQ=255)                                                           acacAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCag                                                                                        <  2:258583/151‑1 (MQ=255)                                                             acaGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCagag                                                                                      <  2:147005/151‑1 (MQ=255)                                                             acaGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCagag                                                                                      <  2:470258/151‑1 (MQ=255)                                                                  tgatgaTATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGatt                                                                                 >  1:434190/1‑151 (MQ=255)                                                                  tgatgaTATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGatt                                                                                 >  1:326268/1‑151 (MQ=255)                                                                      gatATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGatttat                                                                              <  1:384062/150‑1 (MQ=255)                                                                                 tCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAAtgtg                                                                   <  2:297650/150‑1 (MQ=255)                                                                                    aaTTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCttt                                                               >  2:234840/1‑151 (MQ=255)                                                                                    aaTTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCttt                                                               <  2:222259/151‑1 (MQ=255)                                                                                    aaTTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCttt                                                               >  1:204369/1‑151 (MQ=255)                                                                                    aaTTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCtt                                                                <  2:636020/150‑1 (MQ=255)                                                                                     attattAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTg                                                              <  1:49691/151‑1 (MQ=255)                                                                                        attaAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGttt                                                           <  1:181612/151‑1 (MQ=255)                                                                                           aaGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTaa                                                         <  2:570256/150‑1 (MQ=255)                                                                                             gTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTaaaa                                                       >  1:153465/1‑150 (MQ=255)                                                                                               ataAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAAt                                                     <  2:601639/150‑1 (MQ=255)                                                                                                 aaGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACa                                                                                                          >  2:152196/1‑95 (MQ=255)                                                                                                 aaGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACa                                                                                                          <  1:152192/95‑1 (MQ=255)                                                                                                  aGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCg                                                 <  2:572575/151‑1 (MQ=255)                                                                                                      tataGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCgaga                                              <  1:140153/150‑1 (MQ=255)                                                                                                      tataGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCgag                                               >  1:505779/1‑149 (MQ=255)                                                                                                        taGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAgaggg                                           >  1:54045/1‑151 (MQ=255)                                                                                                             aaCTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGgtg                                      >  2:146080/1‑151 (MQ=255)                                                                                                               cTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGtt                                    >  1:393797/1‑151 (MQ=255)                                                                                                                   ttcatCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATACCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGTTCATg                                >  1:601871/1‑151 (MQ=255)                                                                                                                     catCTTATTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGTTCATGAc                              >  1:569561/1‑151 (MQ=255)                                                                                                                           aTTTTTGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGTTCATGACGCATgg                        <  2:4546/151‑1 (MQ=255)                                                                                                                               ttGTCTGTCGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGTTCATGACGCATGGGTAt                    >  2:535609/1‑151 (MQ=255)                                                                                                                                tgtctgtcGCTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATg                   >  1:104513/1‑151 (MQ=255)                                                                                                                                         cTTTAGACTATGCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTc          >  1:650410/1‑151 (MQ=255)                                                                                                                                            tAGACTATGCACAGCATAATTTTATTTGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGTTCATGACGCATCGGTATGTTGATAGTCat        <  1:37851/150‑1 (MQ=255)                                                                                                                                                 tatgCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATattatt  >  1:637247/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ACAAGTGATATTCTCAAAGCTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATT  >  NC_000913/1216558‑1216852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑