Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,297,386	A→G	C246R (TGT→CGT)	adhE ←	fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,297,386	0	A	G	100.0%	119.4 / NA	32	C246R (TGT→CGT)	adhE	fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/12);  total (20/12)

CGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTT  >  NC_000913/1297242‑1297523                                                                                                                                                 |                                                                                                                                          cgTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTAc                                                                                                                                     >  2:204004/1‑151 (MQ=255)                  ttCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGttt                                                                                                                    >  2:557197/1‑151 (MQ=255)                    cAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTg                                                                                                                  <  2:659654/151‑1 (MQ=255)                     aGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTgg                                                                                                                 >  1:354507/1‑151 (MQ=255)                        aTAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGAc                                                                                                               >  1:296028/1‑150 (MQ=255)                        aTAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACa                                                                                                              <  2:318324/151‑1 (MQ=255)                        aTAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACa                                                                                                              <  1:63651/151‑1 (MQ=255)                           aCATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATc                                                                                                            >  2:252364/1‑150 (MQ=255)                             aTCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGt                                                                                                         >  2:229501/1‑151 (MQ=255)                                   aaCAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGAt                                                                                                   <  2:663203/151‑1 (MQ=255)                                        cTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAAc                                                                                              >  2:415678/1‑151 (MQ=255)                                                   ttACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGttt                                                                                     <  2:46706/149‑1 (MQ=255)                                                   ttACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGtt                                                                                      <  2:210079/148‑1 (MQ=255)                                                       ccTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATc                                                                               >  2:579452/1‑151 (MQ=255)                                                       ccTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATc                                                                               >  1:577599/1‑151 (MQ=255)                                                           cAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATcagc                                                                            >  2:400077/1‑150 (MQ=255)                                                                      cgccgTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGAt                                                                <  1:136047/151‑1 (MQ=255)                                                                          gTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATaac                                                             >  2:229861/1‑150 (MQ=255)                                                                            gggTTGCAAAACGTTCACATACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATaacaac                                                          <  1:121057/151‑1 (MQ=255)                                                                                tGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTgg                                                       <  1:675251/150‑1 (MQ=255)                                                                                      aCGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTg                                                <  1:359170/151‑1 (MQ=255)                                                                                              gTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGcc                                        >  1:653050/1‑151 (MQ=255)                                                                                               tACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCt                                       >  2:442518/1‑151 (MQ=255)                                                                                                  aGCGTCATAAACAGAGTCAACAACAACAACATGCTCTTCAGCACCACGGATTACGCCGTCGTCGAAGGTTTTGAACATCAGAACAGATACAACTGCACGTTTGATATCAGCATCTTCATTGATAACAACTGGGGTGCTGCCCGCGccacc                                     >  2:257687/1‑147 (MQ=255)                                                                                                       cATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGAt                               >  2:487750/1‑151 (MQ=255)                                                                                                        aTAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGAt                               >  2:423084/1‑150 (MQ=255)                                                                                                             cAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTgg                         >  1:515947/1‑151 (MQ=255)                                                                                                                agTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGttt                      <  2:544123/151‑1 (MQ=255)                                                                                                                   agtcaacaacaacaGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTAcc                   <  1:686138/148‑1 (MQ=255)                                                                                                                    aacaacaacaacAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCg                  >  1:339961/1‑151 (MQ=255)                                                                                                                               caGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGc       >  1:232525/1‑151 (MQ=255)                                                                                                                                    tGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCtt  >  2:221266/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                          CGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTT  >  NC_000913/1297242‑1297523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑