Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,307,916	C→T	D244N (GAT→AAT)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,916	0	C	T	100.0%	147.1 / NA	38	D244N (GAT→AAT)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/20);  total (18/20)

CGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAG  >  NC_000913/1307786‑1308063                                                                                                                                   |                                                                                                                                                    cGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCgg                                                                                                                                 >  1:257068/1‑151 (MQ=255)   ttttCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGgtg                                                                                                                               >  2:397607/1‑151 (MQ=255)             tCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATg                                                                                                                      <  2:336557/150‑1 (MQ=255)                    cccAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCGCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGAGATGTAATTAt                                                                                                               <  1:483001/150‑1 (MQ=255)                     ccAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCg                                                                                                             <  1:515207/151‑1 (MQ=255)                      cAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATc                                                                                                              >  1:323853/1‑149 (MQ=255)                        gTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatc                                                                                                          <  1:571207/151‑1 (MQ=255)                          cGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatca                                                                                                         <  2:218260/150‑1 (MQ=255)                             aGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcatgat                                                                                                     <  1:581737/151‑1 (MQ=255)                                 ataaataaTCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGatcatc                                                                                                 >  1:486478/1‑151 (MQ=255)                                  taaataaTCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCt                                                                                                >  1:12399/1‑151 (MQ=255)                                       aatCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCg                                                                                            <  2:488689/150‑1 (MQ=255)                                            ccATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTg                                                                                      >  1:331894/1‑151 (MQ=255)                                               tCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGgcg                                                                                   >  2:584988/1‑151 (MQ=255)                                                          aTAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATAc                                                                        <  2:625150/151‑1 (MQ=255)                                                             gggCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGc                                                                     <  1:512577/151‑1 (MQ=255)                                                              ggCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCg                                                                    >  2:355201/1‑151 (MQ=255)                                                                  ttCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTaa                                                                 >  1:224877/1‑150 (MQ=255)                                                                   tCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTaaa                                                                >  2:99577/1‑150 (MQ=255)                                                                         cgcgCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAacacac                                                         <  1:396274/151‑1 (MQ=255)                                                                         cgcgCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAacacac                                                         <  1:342634/151‑1 (MQ=255)                                                                           cgcCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGc                                                       >  1:116770/1‑151 (MQ=255)                                                                               aTCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATc                                                    >  2:394005/1‑150 (MQ=255)                                                                                  aGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATc                                                    >  2:235303/1‑147 (MQ=255)                                                                                          ccATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCttt                                        <  2:686459/151‑1 (MQ=255)                                                                                              tGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAg                                     <  1:417530/150‑1 (MQ=255)                                                                                                   tACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCg                               >  2:495106/1‑151 (MQ=255)                                                                                                    aCGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTc                                <  2:409186/149‑1 (MQ=255)                                                                                                       cccGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCa                           <  1:408895/151‑1 (MQ=255)                                                                                                         cGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACt                         >  1:346357/1‑151 (MQ=255)                                                                                                          gCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACtt                        >  2:551907/1‑151 (MQ=255)                                                                                                               ttGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAAt                    >  2:643375/1‑150 (MQ=255)                                                                                                               ttGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAAt                    >  1:524084/1‑150 (MQ=255)                                                                                                                tGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAAt                    <  1:320171/149‑1 (MQ=255)                                                                                                                    tGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCgg               <  1:577996/150‑1 (MQ=255)                                                                                                                            cGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCa      <  1:655025/151‑1 (MQ=255)                                                                                                                                gATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAg  <  2:626026/151‑1 (MQ=255)                                                                                                                                gATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGAACATGATCAGCTTGCGATGTTGGCGTAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTCCAATACCGGCATTACGCATTa   <  1:143909/150‑1 (MQ=255)                                                                                                                                   |                                                                                                                                                    CGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAG  >  NC_000913/1307786‑1308063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑