Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,312,802	A→T	I30N (ATC→AAC)	yciB ←	IspA family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,312,802	0	A	T	100.0%	193.5 / NA	49	I30N (ATC→AAC)	yciB	IspA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/24);  total (25/24)

AACCTTCCATTTAATAAACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGATCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATC  >  NC_000913/1312657‑1312932                                                                                                                                                  |                                                                                                                                   aaCCTTCCATTTAATAAACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAg                                                                                                                                <  2:478817/150‑1 (MQ=255)           ttAATAAACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGt                                                                                                                       >  1:2274/1‑149 (MQ=255)             aataaACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGcc                                                                                                                   >  2:269781/1‑151 (MQ=255)               taaACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGc                                                                                                                 >  2:538404/1‑151 (MQ=255)                aaaCTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGc                                                                                                                 <  1:538378/150‑1 (MQ=255)                aaaCTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCa                                                                                                                <  1:233332/151‑1 (MQ=255)                  aCTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATa                                                                                                              <  2:488057/151‑1 (MQ=255)                     catcatTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAg                                                                                                             <  2:158285/149‑1 (MQ=255)                     catcatTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTAAGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGAt                                                                                                           <  1:189954/151‑1 (MQ=255)                        catTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTc                                                                                                        >  1:683021/1‑151 (MQ=255)                         atTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCa                                                                                                       <  1:392419/151‑1 (MQ=255)                         atTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCa                                                                                                       <  2:408096/151‑1 (MQ=255)                          ttGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCAt                                                                                                      >  2:488133/1‑151 (MQ=255)                              ggAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAAt                                                                                                  >  2:44088/1‑151 (MQ=255)                                  gaaCAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTg                                                                                              <  1:292278/151‑1 (MQ=255)                                  gaaCAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTg                                                                                              <  1:42352/151‑1 (MQ=255)                                           cAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAAcgcg                                                                                     >  2:58973/1‑151 (MQ=255)                                              gCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGaaa                                                                                  <  1:446803/151‑1 (MQ=255)                                                     aaGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACa                                                                            >  2:416698/1‑150 (MQ=255)                                                        acgacCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAAcc                                                                         >  2:5643/1‑150 (MQ=255)                                                         cgacCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAg                                                                       <  2:70440/151‑1 (MQ=255)                                                           accaccAGAACAAAAGTAATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCg                                                                     <  1:576430/151‑1 (MQ=255)                                                             caccaGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGt                                                                   <  2:351935/151‑1 (MQ=255)                                                             caccaGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGt                                                                   >  2:448552/1‑151 (MQ=255)                                                              accaGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTa                                                                  <  1:318299/151‑1 (MQ=255)                                                                caGAACAAAAGTGATCAGGGCCATCTTCTCATCCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAACCGCGAAAAAGACAACCAGCGGTa                                                                  <  1:89764/149‑1 (MQ=255)                                                                caGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTaa                                                                 >  1:578818/1‑150 (MQ=255)                                                                 aGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTaaa                                                                >  1:663786/1‑150 (MQ=255)                                                                      aaaaGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATc                                                           <  2:145691/150‑1 (MQ=255)                                                                      aaaaGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCa                                                          <  2:414635/151‑1 (MQ=255)                                                                                aGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCt                                                >  2:508973/1‑151 (MQ=255)                                                                                   gCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCa                                             <  2:629450/151‑1 (MQ=255)                                                                                    ccATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCAt                                            <  1:181902/151‑1 (MQ=255)                                                                                      aTCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCAttt                                          >  1:452780/1‑151 (MQ=255)                                                                                          tctcAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCAttt                                          <  2:299359/147‑1 (MQ=255)                                                                                             cAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGAtt                                   >  2:129731/1‑151 (MQ=255)                                                                                             cAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGAtt                                   >  1:680095/1‑151 (MQ=255)                                                                                                ccTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCg                                >  1:165988/1‑151 (MQ=255)                                                                                                  ttACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGt                               >  2:298009/1‑150 (MQ=255)                                                                                                     cGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATc                           >  1:553746/1‑151 (MQ=255)                                                                                                      gAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATc                           >  1:494483/1‑150 (MQ=255)                                                                                                      gAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATc                           >  2:198691/1‑150 (MQ=255)                                                                                                            gAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCt                    <  2:585849/151‑1 (MQ=255)                                                                                                                     tataGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTAt           >  1:673347/1‑151 (MQ=255)                                                                                                                      ataGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTa            <  2:195465/149‑1 (MQ=255)                                                                                                                        aGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTg         <  1:493140/150‑1 (MQ=255)                                                                                                                           tAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCg      >  2:347587/1‑150 (MQ=255)                                                                                                                            aaGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGa     >  2:358693/1‑150 (MQ=255)                                                                                                                              gCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATc  >  1:481763/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                   AACCTTCCATTTAATAAACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGATCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATC  >  NC_000913/1312657‑1312932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑