Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323,187	C→T	intergenic (‑105/‑33)	trpL ← / → yciV	trp operon leader peptide/PHP domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323,187	0	C	T	100.0%	125.9 / NA	34	intergenic (‑105/‑33)	trpL/yciV	trp operon leader peptide/PHP domain protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/14);  total (20/14)

AAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGCGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGC  >  NC_000913/1323043‑1323333                                                                                                                                                 |                                                                                                                                                   aaGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCaaa                                                                                                                                               >  2:17158/1‑150 (MQ=255)           ccaACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATcc                                                                                                                                    <  2:363844/151‑1 (MQ=255)                       tACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTg                                                                                                                        >  2:488093/1‑151 (MQ=255)                           aaaaTTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGcc                                                                                                                    >  2:559982/1‑151 (MQ=255)                              aTTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTg                                                                                                                 <  2:462984/151‑1 (MQ=255)                                                  ccTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGt                                                                                             >  1:148543/1‑151 (MQ=255)                                                       ttACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGAttt                                                                                        >  1:185298/1‑151 (MQ=255)                                                       ttACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGAtt                                                                                         <  2:444404/150‑1 (MQ=255)                                                          cGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACg                                                                                     <  2:147758/151‑1 (MQ=255)                                                              aaCTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCt                                                                                 <  1:175054/151‑1 (MQ=255)                                                                 ttGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGc                                                                               >  1:538277/1‑150 (MQ=255)                                                                   gCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGcac                                                                             <  1:567015/150‑1 (MQ=255)                                                                    cGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAg                                                                           <  1:253565/151‑1 (MQ=255)                                                                             tAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCTAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACcac                                                                  >  2:27043/1‑151 (MQ=255)                                                                               aCTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACcac                                                                  >  2:270558/1‑149 (MQ=255)                                                                               aCTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACcac                                                                  >  1:4898/1‑149 (MQ=255)                                                                                       gatgatTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGAt                                                        >  1:93621/1‑151 (MQ=255)                                                                                            ttAATTGTCAACAGCTCATTTCAGAATATTTGCAAGCACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTg                                                   >  1:392837/1‑151 (MQ=255)                                                                                                     aaCAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGcc                                          <  2:417489/151‑1 (MQ=255)                                                                                                      aCAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATTTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGGCCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCa                                         <  1:98008/151‑1 (MQ=255)                                                                                                      aCAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGc                                           >  1:132094/1‑149 (MQ=255)                                                                                                            cATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGc                                    >  2:427159/1‑150 (MQ=255)                                                                                                              tttCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCAtt                                 >  1:504104/1‑151 (MQ=255)                                                                                                               ttCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCAtt                                 <  1:624549/150‑1 (MQ=255)                                                                                                                tCAGAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTg                                <  2:33185/150‑1 (MQ=255)                                                                                                                   gAATATTTGCCAGAACCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGc                            >  2:563942/1‑151 (MQ=255)                                                                                                                              aGAACCGATATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGAGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGt                  >  1:477032/1‑150 (MQ=255)                                                                                                                                aaCCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCga               <  1:468570/151‑1 (MQ=255)                                                                                                                                aaCCGTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCg                >  1:143210/1‑150 (MQ=255)                                                                                                                                    gTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATg           >  1:127562/1‑151 (MQ=255)                                                                                                                                    gTTATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATg           >  1:347849/1‑151 (MQ=255)                                                                                                                                      tATGATGTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATgcg         >  1:363470/1‑151 (MQ=255)                                                                                                                                          atgTCGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTc     <  1:652446/151‑1 (MQ=255)                                                                                                                                              cGGTGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGc  <  1:44565/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   AAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATATTTGCCAGAACCGTTATGATGTCGGCGCAAAAAACATTATCCAGAACGGGAGTGCGCCTTGAGCGACACGAATTATGCAGTGATTTACGACCTGCACAGCCATACCACAGCTTCCGATGGCTGCCTGACGCCAGAAGCATTGGTGCACCGTGCAGTCGAGATGCGCGTCGGC  >  NC_000913/1323043‑1323333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑