Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,390,211 C→T D130N (GAC→AAC)  mpaA ← murein peptide amidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,390,2110CT100.0% 219.4 / NA 56D130N (GAC→AAC) mpaAmurein peptide amidase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/29);  total (27/29)

AGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGTAACCCCAACTGGCAGCCGTCAG  >  NC_000913/1390075‑1390357
                                                                                                                                        |                                                                                                                                                  
agccaTTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATa                                                                                                                                      <  2:541433/151‑1 (MQ=255)
       cGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATc                                                                                                                               <  1:315313/151‑1 (MQ=255)
          cTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATcgcg                                                                                                                            >  2:686374/1‑151 (MQ=255)
          cTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATcgcg                                                                                                                            >  1:192337/1‑151 (MQ=255)
           tAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGt                                                                                                                           >  1:131221/1‑151 (MQ=255)
            aaTTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGtt                                                                                                                          >  1:104582/1‑151 (MQ=255)
                 gCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCa                                                                                                                     <  1:84415/151‑1 (MQ=255)
                 gCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCa                                                                                                                     <  2:577430/151‑1 (MQ=255)
                   tgtgTCGGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGATTGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACATGTCAGTAATACGACATCGCGTTCTTCAgc                                                                                                                   >  1:441946/1‑151 (MQ=255)
                    gtgtCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAg                                                                                                                    <  1:203772/149‑1 (MQ=255)
                               cTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGGTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTcc                                                                                                        >  1:259174/1‑150 (MQ=255)
                                ttCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTcc                                                                                                        >  2:309915/1‑149 (MQ=255)
                                 tCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCa                                                                                                       <  1:676669/149‑1 (MQ=255)
                                 tCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCa                                                                                                       <  1:118151/149‑1 (MQ=255)
                                  cAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCg                                                                                                    >  2:528025/1‑151 (MQ=255)
                                         ggCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACg                                                                                             <  2:21676/151‑1 (MQ=255)
                                                ggATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCa                                                                                       <  1:253957/150‑1 (MQ=255)
                                                            gagaCCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGt                                                                          <  2:618334/151‑1 (MQ=255)
                                                            gagaCCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGt                                                                          >  1:99884/1‑151 (MQ=255)
                                                                ccaccCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTGCCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGtt                                                                         <  1:286110/148‑1 (MQ=255)
                                                                ccaccCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTcgc                                                                      >  2:7519/1‑151 (MQ=255)
                                                                ccaccCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGGTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTcg                                                                       >  2:338541/1‑150 (MQ=255)
                                                                 caccCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTcgcc                                                                     >  1:103475/1‑151 (MQ=255)
                                                                 caccCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTcgcc                                                                     <  1:553610/151‑1 (MQ=255)
                                                                  accCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTcgcc                                                                     >  1:385017/1‑150 (MQ=255)
                                                                   cccAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTcgccg                                                                    <  1:116972/150‑1 (MQ=255)
                                                                      aggcaggTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCgg                                                                >  1:53169/1‑151 (MQ=255)
                                                                      aggcaggTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCgg                                                                <  1:19232/151‑1 (MQ=255)
                                                                       ggcaggTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGccgccg                                                                 >  1:637215/1‑149 (MQ=255)
                                                                          aggTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGaaa                                                             <  1:17498/150‑1 (MQ=255)
                                                                             ttGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGttt                                                         >  2:577607/1‑151 (MQ=255)
                                                                               gAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCt                                                       <  2:584759/151‑1 (MQ=255)
                                                                                aaTGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTg                                                      >  1:145243/1‑151 (MQ=255)
                                                                                   gCGGTGTATAAGCTGGCGCAGCGCCTGGGTTGCAGGTTAGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGttt                                                   <  1:404900/151‑1 (MQ=255)
                                                                                    cGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTa                                                  <  1:264574/151‑1 (MQ=255)
                                                                                     ggTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTaa                                                 <  2:88058/151‑1 (MQ=255)
                                                                                         tataAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATcc                                             >  2:208700/1‑151 (MQ=255)
                                                                                            aaGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCcac                                           <  1:39098/150‑1 (MQ=255)
                                                                                              gCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCacac                                         <  2:55660/150‑1 (MQ=255)
                                                                                                tGGCACAGCCCCCGGGTTTCAGGTTCGGAGCCCGGTTTCTTACCTGTCAGTAATACGACATCGCGTTCTTAAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCAt                                      >  1:502997/1‑151 (MQ=255)
                                                                                                  gCACAGCGTCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTTTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCTCCGGAAAGTTTCTGTTTAAATCCACACCa                                       <  1:487116/148‑1 (MQ=255)
                                                                                                  gCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTa                                    <  2:252369/151‑1 (MQ=255)
                                                                                                   cacaGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAg                                   <  1:416775/151‑1 (MQ=255)
                                                                                                      aGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCAt                                >  1:664006/1‑151 (MQ=255)
                                                                                                        cgcCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTg                              >  2:556394/1‑151 (MQ=255)
                                                                                                         gcCTGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTgg                             >  2:32778/1‑151 (MQ=255)
                                                                                                            tGGGTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGccc                          >  2:338925/1‑151 (MQ=255)
                                                                                                               gTTTCAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGTa                       <  2:192340/151‑1 (MQ=255)
                                                                                                                   cAGGTTCGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGTAAccc                   >  1:450198/1‑151 (MQ=255)
                                                                                                                         cGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGTAACCCCAACTg             <  1:601775/151‑1 (MQ=255)
                                                                                                                         cGGAGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGTAACCCCAACTg             >  2:672106/1‑151 (MQ=255)
                                                                                                                            aGCCAGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGTAACCCCAACTGGc           >  1:444030/1‑150 (MQ=255)
                                                                                                                                aGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGTAACCCCAACTGGCAGCCg      <  2:362405/151‑1 (MQ=255)
                                                                                                                                aGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGTAACCCCAACTGGCAGCCg      <  1:257928/151‑1 (MQ=255)
                                                                                                                                aGGTTTGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGTAACCCCAACTGGCAGCCg      >  1:216245/1‑151 (MQ=255)
                                                                                                                                     tGTTACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGTAACCCCAACTGGCGGCCGTCAg  <  2:189253/150‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                  
AGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCTGGGTTTCAGGTTCGGAGCCAGGTTTGTCACCTGTCAGTAATACGACATCGCGTTCTTCAGCGGCGCTGTTCCAGCGATAAACGGTTTCACCTTCCTTCCAGTTCGCCGCCGGAAAGTTTCTGTTTAAATCCACACCATTAGCATTGGCCCGTAACCCCAACTGGCAGCCGTCAG  >  NC_000913/1390075‑1390357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: