Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,664,969	T→C	L155P (CTT→CCT)	dmsD →	twin‑argninine leader‑binding protein for DmsA and TorA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,664,969	0	T	C	100.0%	173.6 / NA	46	L155P (CTT→CCT)	dmsD	twin‑argninine leader‑binding protein for DmsA and TorA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/21);  total (25/21)

CGTCAGTGGATGCGCGAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCTTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCG  >  NC_000913/1664824‑1665116                                                                                                                                                  |                                                                                                                                                    cGTCAGTGGATGCGCGAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCctttt                                                                                                                                                 <  2:219962/150‑1 (MQ=255)   tCAGTGGATGCGCGAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCg                                                                                                                                              >  1:504611/1‑151 (MQ=255)        ggATGCGCGAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGt                                                                                                                                          <  2:504916/150‑1 (MQ=255)        ggATGCGCGAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTc                                                                                                                                         >  1:444204/1‑151 (MQ=255)             cgcgAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAacac                                                                                                                                    >  2:60197/1‑151 (MQ=255)              gcgAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACg                                                                                                                                   <  1:475968/151‑1 (MQ=255)                  gaAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGtttt                                                                                                                               >  1:682730/1‑151 (MQ=255)                     aGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCtt                                                                                                                            <  2:546444/151‑1 (MQ=255)                           tCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGt                                                                                                                       <  2:512305/150‑1 (MQ=255)                           tCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGt                                                                                                                       <  1:224079/150‑1 (MQ=255)                             aGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGtttt                                                                                                                    <  1:359373/151‑1 (MQ=255)                                   aaaTGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGa                                                                                                              <  2:627426/151‑1 (MQ=255)                                       gAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGaaaaa                                                                                                          <  1:654230/151‑1 (MQ=255)                                       gAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGaaaa                                                                                                           <  1:541156/150‑1 (MQ=255)                                         aGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGc                                                                                                        <  1:8976/151‑1 (MQ=255)                                             aaaCGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGa                                                                                                     >  2:430510/1‑150 (MQ=255)                                                cGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAacac                                                                                                 >  2:327010/1‑151 (MQ=255)                                                   aCCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCt                                                                                              <  2:478003/151‑1 (MQ=255)                                                      ggAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTc                                                                                           >  2:649420/1‑151 (MQ=255)                                                        aaGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCt                                                                                          >  2:432695/1‑150 (MQ=255)                                                          gATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTAcc                                                                                       >  1:616094/1‑151 (MQ=255)                                                           aTCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAAAACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCg                                                                                      >  1:161674/1‑151 (MQ=255)                                                            tCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGt                                                                                     >  2:607951/1‑151 (MQ=255)                                                             cATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTg                                                                                    <  1:4000/151‑1 (MQ=255)                                                             cATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTg                                                                                    >  1:97423/1‑151 (MQ=255)                                                               ttttGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGc                                                                                   >  2:337935/1‑150 (MQ=255)                                                                        aCTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGa                                                                          >  1:284367/1‑150 (MQ=255)                                                                            ctgTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTgg                                                                     >  1:652095/1‑151 (MQ=255)                                                                                 gATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGc                                                                <  1:440515/151‑1 (MQ=255)                                                                                    ggCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCtt                                                              >  2:589084/1‑150 (MQ=255)                                                                                      cTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTACCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAAc                                                           >  2:479955/1‑151 (MQ=255)                                                                                       tGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACg                                                          >  2:636387/1‑151 (MQ=255)                                                                                            ggTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGgc                                                     <  1:148575/151‑1 (MQ=255)                                                                                                          ggTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATcac                                       >  1:28222/1‑151 (MQ=255)                                                                                                          ggTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATca                                        <  2:202151/150‑1 (MQ=255)                                                                                                            tCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATcaca                                      >  2:407014/1‑150 (MQ=255)                                                                                                             cGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACaa                                     <  2:76333/150‑1 (MQ=255)                                                                                                               ccAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTg                                  >  2:150678/1‑151 (MQ=255)                                                                                                               ccAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTg                                  >  2:125340/1‑151 (MQ=255)                                                                                                                   acagaATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTa                               >  2:247315/1‑150 (MQ=255)                                                                                                                          gCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTg                        <  2:304194/150‑1 (MQ=255)                                                                                                                                    gCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTaa              >  2:620509/1‑150 (MQ=255)                                                                                                                                    gCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTaa              <  2:620204/150‑1 (MQ=255)                                                                                                                                      tggcatggcaCTCTTTTCCGTGGTCAACACGGTTTCTTGATGTTTCTATCGAAAAAGCAGAACACCCTTTTCACCGTGCACTGGGTGAACTGGCGCGCTTAACGTTGGCGCAATAGCAATCACAACTGTTAATTCCTGTCACGGTTAAAcc           >  1:137483/1‑151 (MQ=255)                                                                                                                                       ggcatggcaCCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAAcc           <  2:678833/150‑1 (MQ=255)                                                                                                                                       ggcatggcaCCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAAcc           <  2:7744/150‑1 (MQ=255)                                                                                                                                               aCCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCg  <  2:357702/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CGTCAGTGGATGCGCGAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCTTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCG  >  NC_000913/1664824‑1665116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑