Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,617	A→G	D307G (GAC→GGC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,617	0	A	G	100.0%	137.4 / NA	37	D307G (GAC→GGC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/19);  total (18/19)

GCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCG  >  NC_000913/1756472‑1756763                                                                                                                                                  |                                                                                                                                                   gCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAg                                                                                                                                               <  2:671330/151‑1 (MQ=255)     agaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGAt                                                                                                                                           >  1:638921/1‑151 (MQ=255)       aagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATg                                                                                                                                          >  1:286019/1‑150 (MQ=255)        agaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCt                                                                                                                                        <  2:587994/151‑1 (MQ=255)             atgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTg                                                                                                                                   >  1:460343/1‑151 (MQ=255)              tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTgg                                                                                                                                  >  2:434054/1‑151 (MQ=255)               gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGt                                                                                                                                 <  1:139561/151‑1 (MQ=255)                  cGAAAAATGTATCCGTGCACGTAAATTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCAGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGa                                                                                                                               <  1:486704/150‑1 (MQ=255)                  cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGaa                                                                                                                              <  1:22419/151‑1 (MQ=255)                     aaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATcc                                                                                                                           >  2:155203/1‑151 (MQ=255)                      aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCg                                                                                                                          <  2:680309/151‑1 (MQ=255)                      aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCg                                                                                                                          >  2:640285/1‑151 (MQ=255)                        aTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCa                                                                                                                        <  2:672586/151‑1 (MQ=255)                           tATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCaaa                                                                                                                      >  2:543433/1‑150 (MQ=255)                            aTCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCaaaa                                                                                                                     <  2:20742/150‑1 (MQ=255)                            aTCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAg                                                                                                                    <  1:326166/151‑1 (MQ=255)                                 tGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTaa                                                                                                                >  2:188219/1‑150 (MQ=255)                                        aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGc                                                                                                        <  2:342167/151‑1 (MQ=255)                                        aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGc                                                                                                        <  2:410307/151‑1 (MQ=255)                                              gTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGa                                                                                                    >  2:113216/1‑149 (MQ=255)                                                       gCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCt                                                                                          <  1:421918/150‑1 (MQ=255)                                                                aTGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCg                                                                                 >  2:328311/1‑150 (MQ=255)                                                                    tGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGAcc                                                                              >  1:156879/1‑149 (MQ=255)                                                                     ggATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGCAAATACCCGCTGGAAGCGGTTTCTATCATGACGAccct                                                                            >  1:298479/1‑148 (MQ=255)                                                                        ttCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGc                                                                        >  1:303455/1‑151 (MQ=255)                                                                                     aaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTaccgacc                                                           <  1:56861/151‑1 (MQ=255)                                                                                     aaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTaccgacc                                                           <  2:373335/151‑1 (MQ=255)                                                                                                    cgcgCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGcc                                            >  1:346371/1‑151 (MQ=255)                                                                                                       gcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGtc                                         <  1:509378/151‑1 (MQ=255)                                                                                                       gcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGtc                                         >  2:266007/1‑151 (MQ=255)                                                                                                         aGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGtctc                                       >  2:487459/1‑151 (MQ=255)                                                                                                                         gCAAACGCCATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGAc                        <  1:131735/150‑1 (MQ=255)                                                                                                                                 cATCCTCGACGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGACTCGAGTTCAACAATGACAACCGTaaa               <  2:663127/151‑1 (MQ=255)                                                                                                                                         aCGGTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCAt       <  2:209717/151‑1 (MQ=255)                                                                                                                                           ggTACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTa     <  2:477478/151‑1 (MQ=255)                                                                                                                                             tACTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAcc   >  2:183997/1‑151 (MQ=255)                                                                                                                                               cTGGCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCg  >  1:46040/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                   GCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCG  >  NC_000913/1756472‑1756763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑