Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,769,479	A→G	T136A (ACG→GCG)	ydiK →	UPF0118 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,769,479	0	A	G	100.0%	140.2 / NA	41	T136A (ACG→GCG)	ydiK	UPF0118 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/25);  total (16/25)

TCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACTGGCGCGGTG  >  NC_000913/1769339‑1769617                                                                                                                                              |                                                                                                                                           tcgtcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCg                                                                                                                                          >  2:302891/1‑143 (MQ=255)       aCGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGa                                                                                                                             >  2:420635/1‑150 (MQ=255)                  ccGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCtt                                                                                                                 >  1:523178/1‑151 (MQ=255)                   cGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTta                                                                                                                <  1:490087/151‑1 (MQ=255)                    gCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTtat                                                                                                               <  1:479009/151‑1 (MQ=255)                       aaTTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATAtt                                                                                                            <  2:639770/151‑1 (MQ=255)                               ccATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcactac                                                                                                    <  2:687426/151‑1 (MQ=255)                                  tttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGTGATCATGGCGAAAGTCCGCCCTTATATTGGcaccacca                                                                                                  <  1:111702/150‑1 (MQ=255)                                       aGCGGTTACCGGACGTCACCCGATCTGGCGGGGCTTAATACCAGTCCGGAGATGGGAGCGCAGCTGTATGCAGGCGGGCACAACTTGCTGGATAT‑GGCGGGGGCGGCGGTCATGCTGGAACTCCCCGCTTATATTCTTcccaccaccacc                                                                                             >  1:155669/1‑150 (MQ=255)                                         cGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTgg                                                                                          <  1:508599/151‑1 (MQ=255)                                           gTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGtt                                                                                        <  2:680675/151‑1 (MQ=255)                                           gTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGtt                                                                                        <  1:174907/151‑1 (MQ=255)                                                          ccGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAggcggc                                                                         >  2:71086/1‑151 (MQ=255)                                                             aTCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGgcgc                                                                       >  2:623727/1‑150 (MQ=255)                                                             aTCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGgcgc                                                                       >  1:549801/1‑150 (MQ=255)                                                               cTGGCGGGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCat                                                                     >  1:656859/1‑150 (MQ=255)                                                                 ggCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCGCCACCTGGTTCGTTGGGCAggcggc                                                                         <  2:495492/144‑1 (MQ=255)                                                                 ggCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCatat                                                                   <  1:480786/150‑1 (MQ=255)                                                                  gCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCg                                                                 >  2:293977/1‑151 (MQ=255)                                                                     tGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCgg                                                                <  1:402274/149‑1 (MQ=255)                                                                      ggCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgcg                                                             <  1:419070/151‑1 (MQ=255)                                                                      ggCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgcg                                                             >  2:240659/1‑151 (MQ=255)                                                                      ggCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgc                                                              <  1:256273/150‑1 (MQ=255)                                                                           aaTACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGAGCTGCGTTCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTa                                                        <  1:539996/151‑1 (MQ=255)                                                                             tACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATg                                                      >  1:573491/1‑151 (MQ=255)                                                                               ccATTACGACGATTGGCGCGAAGCTGTATGAAGGTTGGCACAACTTGCTGGATAT‑GGCGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATGGGCACCACCGCCACCTGGTGCGTGGGACAGGCGGCGCATATCGGgcgtt                                                           <  1:423411/144‑3 (MQ=255)                                                                                    ccGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCAt                                                <  2:235722/151‑1 (MQ=255)                                                                                              gcgcGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGAt                                     <  2:677440/151‑1 (MQ=255)                                                                                                gcgAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGc                                   >  1:508470/1‑151 (MQ=255)                                                                                                   aaGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTtc                                >  2:317958/1‑151 (MQ=255)                                                                                                     gCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTtctc                              <  2:274173/151‑1 (MQ=255)                                                                                                         tATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCAACACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTc                           >  2:688185/1‑150 (MQ=255)                                                                                                           tGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGt                        >  2:294200/1‑151 (MQ=255)                                                                                                            gCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTg                       <  1:279507/151‑1 (MQ=255)                                                                                                             cAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGc                      <  2:519730/151‑1 (MQ=255)                                                                                                               ggCTGGCACAACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGccc                    <  2:487458/151‑1 (MQ=255)                                                                                                                   ggCACATCTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCCCCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCctgct                <  1:565861/151‑1 (MQ=255)                                                                                                                       caACTTGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGTTTCGTTGGGCAGTCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTa             <  1:127737/150‑1 (MQ=255)                                                                                                                            tGCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTGTGGTGCATTGTGCGCTGCTGCTTCTCTTCAGTGCCCTGCTGTACTGgc        <  1:574704/150‑1 (MQ=255)                                                                                                                             gCTGGATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACTGgcg       <  2:625186/150‑1 (MQ=255)                                                                                                                                 gATAT‑GGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACTGGCGCGGTg  >  1:517493/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                           TCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACTGGCGCGGTG  >  NC_000913/1769339‑1769617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑