Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,870,836	T→C	L151P (CTG→CCG)	yeaI →	putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,870,836	0	T	C	100.0%	170.5 / NA	45	L151P (CTG→CCG)	yeaI	putative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/30);  total (15/30)

CCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTT  >  NC_000913/1870689‑1870977                                                                                                                                                    |                                                                                                                                              ccGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGAt                                                                                                                                            <  2:457022/151‑1 (MQ=255)  cGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATc                                                                                                                                           <  1:343469/151‑1 (MQ=255)         tttcttttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACa                                                                                                                                     >  1:642140/1‑150 (MQ=255)           tcttttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACaaa                                                                                                                                   <  2:198854/150‑1 (MQ=255)              tttcATGTTAATGAAATATTTCACTAACACACAAATTTTACCAACACTCAGTTTAAGCCTTGCATTTTTAAGTGGCCTTGTCGATTTAGTTCAAACCATTGTAAATATCCAAAAGCCAATTAACGGCAGTACACCGATCCAGTCAAAgg                                                                                                                                 <  1:130514/149‑2 (MQ=255)              tttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAg                                                                                                                                  <  2:305809/148‑1 (MQ=255)              tttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAg                                                                                                                                  <  2:364481/148‑1 (MQ=255)              tttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCg                                                                                                                               >  1:180231/1‑151 (MQ=255)                    gTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAatgat                                                                                                                         >  1:393083/1‑151 (MQ=255)                                   cACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctattttctat                                                                                                          >  2:443630/1‑151 (MQ=255)                                       aacaaacaAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATAttt                                                                                                      <  1:324288/151‑1 (MQ=255)                                       aacaaacaAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATAttt                                                                                                      <  1:245651/151‑1 (MQ=255)                                              aaaTTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCa                                                                                               <  2:403184/151‑1 (MQ=255)                                                aTTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAAc                                                                                             >  2:16591/1‑151 (MQ=255)                                                              cATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTAttt                                                                                 <  1:374132/149‑1 (MQ=255)                                                                 tttAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGttt                                                                             >  1:18407/1‑150 (MQ=255)                                                                  ttAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTa                                                                            <  1:86894/150‑1 (MQ=255)                                                                    aaGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAAcc                                                                         <  1:27221/151‑1 (MQ=255)                                                                     aGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCt                                                                        <  2:145007/151‑1 (MQ=255)                                                                          tGCATTTTTAAGTGGCCTTAACTATTTAGTTGAATCCATTGTAATTATCCATAAACCAATTAACGGCAGTGTTCCGATCCAGACAATGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTGTAACCGCGCt                                                                    <  1:595370/150‑1 (MQ=255)                                                                                 ttAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGctct                                                            >  1:504023/1‑151 (MQ=255)                                                                                       ggCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGt                                                       <  2:670326/150‑1 (MQ=255)                                                                                           ttATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATgg                                                  >  2:84063/1‑151 (MQ=255)                                                                                            tATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATgg                                                  >  2:131096/1‑150 (MQ=255)                                                                                            tATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATg                                                   <  2:542705/149‑1 (MQ=255)                                                                                               cTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGaaa                                               >  1:531604/1‑150 (MQ=255)                                                                                                 aTTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGaaa                                               >  2:394111/1‑148 (MQ=255)                                                                                                 aTTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGaa                                                <  1:332006/147‑1 (MQ=255)                                                                                                 aTTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGaa                                                <  1:580806/147‑1 (MQ=255)                                                                                                 aTTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGa                                            <  1:402229/151‑1 (MQ=255)                                                                                                        tGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACAt                                      <  2:374479/150‑1 (MQ=255)                                                                                                         gAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATcc                                    <  1:96823/151‑1 (MQ=255)                                                                                                                 tGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGacaac                            >  1:32602/1‑151 (MQ=255)                                                                                                                 tGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGacaa                             <  2:177037/150‑1 (MQ=255)                                                                                                                   tAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGacaaca                           >  2:225350/1‑150 (MQ=255)                                                                                                                    aaTTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAAt                         <  2:540460/151‑1 (MQ=255)                                                                                                                       tATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATaa                       <  2:224094/150‑1 (MQ=255)                                                                                                                        aTCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGa                     <  2:454791/151‑1 (MQ=255)                                                                                                                          ccATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGaa                    >  1:90059/1‑150 (MQ=255)                                                                                                                            aTAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGaaaaa                 <  1:448398/151‑1 (MQ=255)                                                                                                                               aaCCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAAc              <  2:137531/151‑1 (MQ=255)                                                                                                                                   aaTTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCCATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACggg           >  1:444309/1‑150 (MQ=255)                                                                                                                                      tAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAAt        <  2:530579/150‑1 (MQ=255)                                                                                                                                         cGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTg    <  2:532675/151‑1 (MQ=255)                                                                                                                                           gCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGtt  <  2:429675/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              CCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTT  >  NC_000913/1870689‑1870977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑