Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,214,702	T→C	intergenic (‑58/‑164)	yehU ← / → mlrA	inner membrane putative sensory kinase in two‑component system with YehT/transcriptional activator of csgD and csgBA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,214,702	0	T	C	100.0%	169.0 / NA	42	intergenic (‑58/‑164)	yehU/mlrA	inner membrane putative sensory kinase in two‑component system with YehT/transcriptional activator of csgD and csgBA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/21);  total (21/21)

GAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACCG  >  NC_000913/2214555‑2214840                                                                                                                                                    |                                                                                                                                           gAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAActtcctt                                                                                                                                         <  2:171485/151‑1 (MQ=255)        ggCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAActc                                                                                                                                  >  1:197672/1‑151 (MQ=255)        ggCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAActc                                                                                                                                  <  2:611778/151‑1 (MQ=255)                aCTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGaa                                                                                                                          <  2:304037/151‑1 (MQ=255)                          cATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAAt                                                                                                                <  1:406177/151‑1 (MQ=255)                                          aaaCGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCAc                                                                                                >  1:600605/1‑151 (MQ=255)                                           aaCGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACt                                                                                               >  2:205554/1‑151 (MQ=255)                                               cacaTCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCaaa                                                                                           <  1:159734/151‑1 (MQ=255)                                               cacaTCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTAAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCaaa                                                                                           <  1:162411/151‑1 (MQ=255)                                                     tgctgAAGCAGCCGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGcc                                                                                     >  2:481223/1‑151 (MQ=255)                                                      gctgAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAGCCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATc                                                                                        <  1:417886/147‑1 (MQ=255)                                                      gctgAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATc                                                                                        <  1:84154/147‑1 (MQ=255)                                                      gctgAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATc                                                                                        <  2:86459/147‑1 (MQ=255)                                                        tgAAGCAGCAGCAACACCAGATTAAAATCGTACATACCTGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTgg                                                                                  >  2:451254/1‑151 (MQ=255)                                                             cagcagcaACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCaaaa                                                                             <  1:590364/151‑1 (MQ=255)                                                              agcagcaACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCaaaaa                                                                            <  1:299270/151‑1 (MQ=255)                                                              agcagcaACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCaaaa                                                                             >  2:631321/1‑150 (MQ=255)                                                                cagcaACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAaaaat                                                                           >  1:171270/1‑150 (MQ=255)                                                                 agcaACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAaaaata                                                                          <  2:377286/150‑1 (MQ=255)                                                                  gcaACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAaaaataa                                                                         >  2:569828/1‑150 (MQ=255)                                                                    aaCACCAGATTAAAATCGTACATACCCACAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAaaaataaaat                                                                      >  2:168355/1‑151 (MQ=255)                                                                      cacCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCa                                                                    <  1:674530/151‑1 (MQ=255)                                                                        ccAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCa                                                                                                 <  1:97998/120‑1 (MQ=255)                                                                        ccAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCa                                                                                                 >  2:97999/1‑120 (MQ=255)                                                                           gATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCta                                                               <  1:78337/151‑1 (MQ=255)                                                                                aaaTCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCtat                                                              >  2:119545/1‑147 (MQ=255)                                                                                aaaTCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCtat                                                              >  1:383416/1‑147 (MQ=255)                                                                                  aTCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGc                                                        <  1:390538/151‑1 (MQ=255)                                                                                     gTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGcaca                                                     <  1:145685/151‑1 (MQ=255)                                                                                      tACATACCCGCAAACCACACTTCCCTTTAAAACACGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGcaca                                                     >  2:518819/1‑150 (MQ=255)                                                                                       aCATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACaa                                                    <  2:659171/150‑1 (MQ=255)                                                                                       aCATACCCGCAAACCACACTTCCCTTTAAAACGCGGAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACaa                                                    >  1:610138/1‑150 (MQ=255)                                                                                         aTACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACaaaa                                                  >  2:524630/1‑150 (MQ=255)                                                                                             ccGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACgg                                             >  2:185940/1‑151 (MQ=255)                                                                                                 aaaCCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCaa                                         <  2:372122/151‑1 (MQ=255)                                                                                                       cacTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTAc                                   >  2:194638/1‑151 (MQ=255)                                                                                                         cTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTAcc                                  <  1:362470/150‑1 (MQ=255)                                                                                                                   aaaCGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCaa                        >  1:62184/1‑150 (MQ=255)                                                                                                                    aaCGCGTAACATACATTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCaaaa                      <  1:600675/151‑1 (MQ=255)                                                                                                                            acatacatTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCt              >  1:265060/1‑151 (MQ=255)                                                                                                                                acatTGCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTaaa           <  1:59498/150‑1 (MQ=255)                                                                                                                                     gCCTGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTaa      >  2:54909/1‑150 (MQ=255)                                                                                                                                        tGCGTTTAACTTCCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACCg  >  1:378629/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                           GAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCGTAACATACATTGCCTGCGTTTAACTTTCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACCG  >  NC_000913/2214555‑2214840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑