| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,222,366 | T→C | V61A (GTG→GCG) | dld → | D‑lactate dehydrogenase, FAD‑binding, NADH independent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,222,366 | 0 | T | C | 97.7% | 114.4 / ‑6.6 | 43 | V61A (GTG→GCG) | dld | D‑lactate dehydrogenase, FAD‑binding, NADH independent |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (11/31); minor base G (1/0); total (12/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 | |||||||||||
TTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTC > NC_000913/2222221‑2222512 | ttGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCt > 2:455803/1‑149 (MQ=255) gaatgaaCTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGaaa < 1:162130/151‑1 (MQ=255) aatgaaCTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAg < 1:96317/151‑1 (MQ=255) aatgaaCTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCGGaaa > 1:8890/1‑150 (MQ=255) gaaCTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCGGGAAg > 1:209688/1‑148 (MQ=255) gtggGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACa < 2:304838/151‑1 (MQ=255) cacCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTg < 1:105707/150‑1 (MQ=255) cTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGGCAAAAATATTCTGAt > 2:147343/1‑149 (MQ=255) cACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGCCGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGgcc < 1:125531/151‑1 (MQ=255) cACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGgcc < 2:95718/151‑1 (MQ=255) aCCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAACCTGCGGCACCGCCGACAAAATTATTCTGATGCAGgcc > 2:69327/1‑150 (MQ=255) cGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGgccg‑cc < 2:121175/151‑1 (MQ=255) gATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCCGTTGAAGTCTGCGGCCACCCCGGCCAAAATATTCTTATTGAGgccg‑cc > 1:341938/1‑150 (MQ=255) cGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAACCTGCGTCACCGCCGACAAAATTATTTTGATGCAGGCCG‑CCCATACAGGCCTGAcc > 2:226248/1‑147 (MQ=255) aTCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATc < 2:493614/151‑1 (MQ=255) gggCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑cc < 2:540258/150‑1 (MQ=255) ttCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAAc < 1:430759/150‑1 (MQ=255) ccGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACgg < 2:534615/150‑1 (MQ=255) ccGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACg < 2:168262/149‑1 (MQ=255) ggTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACg < 1:533766/148‑1 (MQ=255) ggTAAGGGCGACGCGCTGGCTGTCGTTTCCCCTGGCTCCCTCCTAGAATTGTGGCGGGCGCTGAAAGCTTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACg < 1:657615/148‑1 (MQ=255) aGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTAt < 1:201095/149‑1 (MQ=255) aGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGa < 2:338361/151‑1 (MQ=255) gACGCGCTGGCTGTCTTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATcg < 2:233160/149‑1 (MQ=255) gACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTTAAAACCTGCGTCACCGCCGACAAAATTATTCTGATGCAAGCCG‑CCAATACAAGCCTGACCGAAGGATCGACG‑CCCAACGGTAAACATTAttaac > 2:3217/1‑144 (MQ=255) gACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATcgc < 2:217394/150‑1 (MQ=255) gcgcTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATg < 2:343398/151‑1 (MQ=255) ggctgTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGtt < 2:349001/151‑1 (MQ=255) ggctgTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGt < 2:55212/150‑1 (MQ=255) ctgTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGTTa < 1:398131/150‑1 (MQ=255) ctcttgTTTTCCCTGCCTCACTACTAGAATTGTGGCGGGCGCTGAAGGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGt < 1:138749/143‑1 (MQ=255) tgTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCCCCGACAAAATTATTTTTATGCAGGCCGCCCAAAACA‑GCCTGACCGAAGGGGCGACGCCCAAACGG‑AACGATTATTATTCCGACGGCGTTat > 1:677877/1‑150 (MQ=255) tttCCCTGGCTCACTACTAGAATTGTGGCGGGGGCTGCAAGCCTTCGTCACCCCCGGCCACATTATTTTTTTGAAAGCCG‑CCCAAAAAAGCCCGATCGCAAGAACGACG‑CCCAAAGGAAAACATTATGGTCGCGGTTTCGtttttttcaa > 1:579384/1‑142 (MQ=255) tttCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAg < 2:114460/150‑1 (MQ=255) ttCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGc < 1:170295/150‑1 (MQ=255) ggCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCt < 1:289516/149‑1 (MQ=255) cTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCTCCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCt < 2:418920/147‑1 (MQ=255) actaGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGAc < 1:384254/151‑1 (MQ=255) aGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACa < 1:631567/149‑1 (MQ=255) aaTTGTGGCGGGCGCTGAAAGCCGGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCt < 1:163802/151‑1 (MQ=255) aaTTGTGGCGGGCGCCGAAAACCCGCGTCACCGCCCCCAAAAATATTTTGATGCAGGCCG‑CCAATACAAGGCCGGCCGAAGGATCGGCG‑CCAAACGGGAACGATTATTATCCCGATGTCCTTATCATCACCACCCCGCGCCTCGGCaaa > 1:270884/1‑148 (MQ=255) cGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTc > 1:294129/1‑151 (MQ=255) gggCGCTGAAAGCTTGCTTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTc < 1:341587/150‑1 (MQ=255) | TTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCG‑CCAATACAGGCCTGACCGAAGGATCGACG‑CCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTC > NC_000913/2222221‑2222512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |