| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,317,917 | T→C | T654A (ACC→GCC) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,317,917 | 0 | T | C | 97.1% | 108.8 / ‑6.8 | 34 | T654A (ACC→GCC) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (11/22); minor base A (1/0); total (12/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
TGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTAT > NC_000913/2317787‑2318061 | tGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCCCAAAGCGCCAGACCCAGACCGTCCCCCTGGAAATTACGCt < 1:507486/148‑1 (MQ=255) gCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTAc < 1:37278/151‑1 (MQ=255) cTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGtt > 2:415572/1‑151 (MQ=255) gcgcGGTACAACGGAATCCGCACGGTAAAGTGGCTGCCCATTCCCGGTTCTGAATTTACCGTGATATCGCCGTCCTTCATTCTGATCAGTTTTTCACAAATCGTGAGACCCAGACCGGCCCCCGGGAAATTGCGCTGTTCGCCGGtt < 1:1278/147‑1 (MQ=255) cGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTg < 1:64328/151‑1 (MQ=255) gTACAACGGAATACACACTGCAAACTCGCTCTCCGGACCCGGATATGAATCTTCCGAGATATCGCCGTCGTTCATGCTGATCAGTTTGTCGCCAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCATTCTGAcca < 1:101120/149‑2 (MQ=255) agcGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGgaaga < 1:379866/149‑1 (MQ=255) aaCGGAATACGCACGGTAGACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGgaaga < 2:623724/151‑1 (MQ=255) aCGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCACCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATc < 1:1232/150‑1 (MQ=255) gCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCaaa < 2:266539/151‑1 (MQ=255) gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCActtc < 1:530579/150‑1 (MQ=255) cATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCActtctg < 1:470770/149‑2 (MQ=255) aTTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCg > 2:354161/1‑151 (MQ=255) cGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGTCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGt > 2:228380/1‑151 (MQ=255) cGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGt < 1:469782/151‑1 (MQ=255) ggTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTGCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTa < 1:358953/151‑1 (MQ=255) gAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATcc < 1:580814/148‑1 (MQ=255) aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACg < 2:529355/147‑1 (MQ=255) gagaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTAt < 1:313875/151‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAaca < 2:272666/151‑1 (MQ=255) gTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACgg > 2:678814/1‑151 (MQ=255) tGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATagag > 2:407574/1‑149 (MQ=255) gATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATa < 2:635436/150‑1 (MQ=255) cAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATc < 1:69891/150‑1 (MQ=255) cAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATc < 2:394158/150‑1 (MQ=255) cacaAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgc > 1:12889/1‑151 (MQ=255) acaAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcg > 1:84304/1‑151 (MQ=255) caAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcgc > 1:567039/1‑151 (MQ=255) aaaTCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcgcg > 1:516472/1‑151 (MQ=255) ccagacccagacCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATg > 1:676911/1‑151 (MQ=255) gacccagaccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCCGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCa > 2:660874/1‑150 (MQ=255) cccagaccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCaaa < 2:107297/150‑1 (MQ=255) accGGACCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtat > 2:413005/1‑150 (MQ=255) ccGGCCCCCTGGAAATTACGCTGTCCGCCAGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACGTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACt < 1:646176/147‑1 (MQ=255) | TGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTAT > NC_000913/2317787‑2318061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |