Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,419,171	T→C	E2E (GAA→GAG)	yfcD ←	putative NUDIX hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,419,171	0	T	C	100.0%	177.9 / NA	46	E2E (GAA→GAG)	yfcD	putative NUDIX hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/29);  total (17/29)

ATTTTGCCCATGCCATCATGCACGACGATGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCAT  >  NC_000913/2419025‑2419316                                                                                                                                                   |                                                                                                                                                  aTTTTGCCCATGCCATCATGCACGACGATGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTcc                                                                                                                                                >  1:481218/1‑150 (MQ=255)     tGCCCATGCCATCATGCACGACGATGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTa                                                                                                                                            <  1:510947/150‑1 (MQ=255)                    gCACGACGATGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACcttctt                                                                                                                             <  1:303023/150‑1 (MQ=255)                       cgacgaTGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTc                                                                                                                         >  1:681218/1‑151 (MQ=255)                       cgacgaTGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTc                                                                                                                         <  2:469577/151‑1 (MQ=255)                             tGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGgcgc                                                                                                                   >  2:255044/1‑151 (MQ=255)                              gTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGct                                                                                                                  <  2:452305/151‑1 (MQ=255)                                aaGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGctct                                                                                                                >  2:402657/1‑151 (MQ=255)                                  gTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTa                                                                                                              <  2:69994/151‑1 (MQ=255)                                   ttGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACcttctt                                                                                                                             <  1:138/135‑1 (MQ=255)                                   ttGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACcttctt                                                                                                                             >  2:138/1‑135 (MQ=255)                                      cACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACATTATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCGTGCgga                                                                                                          <  1:145471/151‑2 (MQ=255)                                               gCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGa                                                                                                 <  1:434024/151‑1 (MQ=255)                                                  gACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAATCGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAg                                                                                                <  1:606094/149‑1 (MQ=255)                                                  gACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGtt                                                                                              <  1:7203/151‑1 (MQ=255)                                                    cacTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTtgt                                                                                            <  2:397756/151‑1 (MQ=255)                                                     acTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTtgtg                                                                                           <  2:574740/151‑1 (MQ=255)                                                          gCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTaa                                                                                      <  1:37893/151‑1 (MQ=255)                                                                  ttGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACgg                                                                               >  2:671847/1‑150 (MQ=255)                                                                     ttCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGAtt                                                                            >  1:665004/1‑150 (MQ=255)                                                                          ggctggctTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATcgc                                                                      >  1:292909/1‑151 (MQ=255)                                                                               gctTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCt                                                                 <  1:644278/151‑1 (MQ=255)                                                                                ctTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTg                                                                >  2:447929/1‑151 (MQ=255)                                                                                              tCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACttt                                                  >  2:90192/1‑151 (MQ=255)                                                                                               cGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACttt                                                  <  2:121343/150‑1 (MQ=255)                                                                                               cGTTCTCTTCATTGACAATATCCACCCATTCAGTACGTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTTTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTg                                                 <  1:213036/151‑1 (MQ=255)                                                                                                       tCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGTAAACCTTCTTTTTCTGGCGCTCTTACGTCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCAt                                            <  1:347556/148‑1 (MQ=255)                                                                                                       tCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCAtt                                           <  1:455851/149‑1 (MQ=255)                                                                                                       tCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTg                                          >  1:165793/1‑150 (MQ=255)                                                                                                        cATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAg                                        <  1:446901/151‑1 (MQ=255)                                                                                                         aTTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGt                                       <  2:69739/151‑1 (MQ=255)                                                                                                               aaTATCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGAtt                                 <  1:255040/151‑1 (MQ=255)                                                                                                                   tCCACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTacac                             >  2:117589/1‑151 (MQ=255)                                                                                                                     cACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACt                            >  2:370534/1‑150 (MQ=255)                                                                                                                     cACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACt                            >  2:500624/1‑150 (MQ=255)                                                                                                                     cACCCATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTAAGTGCGATTACACt                            >  1:283833/1‑150 (MQ=255)                                                                                                                       cccATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAg                         <  1:500653/151‑1 (MQ=255)                                                                                                                       cccATTCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTGTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTaa                          <  1:487132/150‑1 (MQ=255)                                                                                                                            tCAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACg                     <  2:178044/150‑1 (MQ=255)                                                                                                                             cAGTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTc                   <  1:276609/151‑1 (MQ=255)                                                                                                                               gTACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCAt                 <  2:295730/151‑1 (MQ=255)                                                                                                                                tACTTGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCAtt                >  2:631675/1‑151 (MQ=255)                                                                                                                                    tGCCAAACGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATcc            >  1:554403/1‑151 (MQ=255)                                                                                                                                         aaCGACGCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCAt       <  1:665492/151‑1 (MQ=255)                                                                                                                                             acgCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCa   <  2:555030/151‑1 (MQ=255)                                                                                                                                              cgCTGCTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCa   <  1:473017/150‑1 (MQ=255)                                                                                                                                               gctgctCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCAt  >  2:663207/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ATTTTGCCCATGCCATCATGCACGACGATGTAAGTTGCACGATGACGCAGACACTGTGCCCGCATTTGTTCCCGGCTGGCTTGTGCAATGACTTCGTTCTCTTCATTGACAATATCCACCCATTCAGTACTTGCCAAACGACGCTGTTCCATTATCAGGAAACCTTCTTTTTCTGGCGCTCTTACGGCGCATTTTGAGTTGTGGGTAAATTACGGATTAATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCAT  >  NC_000913/2419025‑2419316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑