Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,688,959	A→G	V183A (GTT→GCT)	yfhG ←	putative outer membrane protein modulating the QseEF response

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,688,959	0	A	G	100.0%	148.9 / NA	38	V183A (GTT→GCT)	yfhG	putative outer membrane protein modulating the QseEF response
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/17);  total (21/17)

GGATGGCGCGGGCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAACGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGAACCTGTGCCGGGATC  >  NC_000913/2688823‑2689105                                                                                                                                         |                                                                                                                                                   ggATGGCGCGGGCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTgg                                                                                                                                      <  1:575280/151‑1 (MQ=255)  gATGGCGCGGGCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGgc                                                                                                                                     <  2:389996/151‑1 (MQ=255)     ggCGCGGGCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCa                                                                                                                                  <  2:69370/151‑1 (MQ=255)         cgGGCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGt                                                                                                                              >  2:368959/1‑151 (MQ=255)            gCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGtctc                                                                                                                           >  1:41340/1‑151 (MQ=255)                ttCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAg                                                                                                                        <  2:50103/150‑1 (MQ=255)                     tctcGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCtcgct                                                                                                                  >  2:308598/1‑151 (MQ=255)                            cggcgTAGCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCtcgctgtcgctg                                                                                                           <  1:69381/151‑1 (MQ=255)                               cgTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGAc                                                                                                        <  1:125757/151‑1 (MQ=255)                                gTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGAct                                                                                                       <  2:251398/151‑1 (MQ=255)                                  aTCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGActgc                                                                                                     >  2:67512/1‑151 (MQ=255)                                  aTCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGActg                                                                                                      <  2:267847/150‑1 (MQ=255)                                   tCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGActgct                                                                                                    >  2:53938/1‑151 (MQ=255)                                     cGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTgg                                                                                                  >  2:306667/1‑151 (MQ=255)                                         gagaAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGtt                                                                                              <  2:5055/151‑1 (MQ=255)                                             aaTTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACt                                                                                          >  1:422496/1‑151 (MQ=255)                                              aTTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTg                                                                                         <  2:201108/151‑1 (MQ=255)                                                        ggTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGGCTGCTGGAGTTTACTGTAACGTTGgc                                                                               >  1:179295/1‑151 (MQ=255)                                                                    gagaGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGcc                                                                    >  2:327427/1‑150 (MQ=255)                                                                            gCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCcagttgcagt                                                           >  2:630974/1‑151 (MQ=255)                                                                                  aaTATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAgc                                                     >  2:301318/1‑151 (MQ=255)                                                                                    tatCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAgcgc                                                   >  2:121844/1‑151 (MQ=255)                                                                                    tatCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAgc                                                     >  1:403635/1‑149 (MQ=255)                                                                                     atCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGcc                                                  <  1:12257/151‑1 (MQ=255)                                                                                       cGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCt                                                 <  2:528724/150‑1 (MQ=255)                                                                                       cGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTg                                                >  2:157269/1‑151 (MQ=255)                                                                                           cAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGAcc                                             <  1:586672/150‑1 (MQ=255)                                                                                              gTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATcg                                         <  2:175622/151‑1 (MQ=255)                                                                                                         tGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGtt                               <  1:458575/150‑1 (MQ=255)                                                                                                          gCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTg                              >  1:399035/1‑150 (MQ=255)                                                                                                           cGGGTGGTGAGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGAt                            >  1:290176/1‑151 (MQ=255)                                                                                                                    aGTTCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGc                   >  1:166675/1‑151 (MQ=255)                                                                                                                      ttCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCg                  >  1:227388/1‑150 (MQ=255)                                                                                                                       tCCAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGa                 <  1:351962/150‑1 (MQ=255)                                                                                                                         cAGTTGCTGCTGTAGAGCGTGATGTTGCTGGCGCAATGTAGCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTGGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGAAcc              <  1:138721/151‑1 (MQ=255)                                                                                                                                tgctgTAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGAACCTGTGCCg       >  2:141987/1‑151 (MQ=255)                                                                                                                                     tAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGAACCTGTGCCGGGAtc  >  2:375963/1‑151 (MQ=255)                                                                                                                                     tAGAGCGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGAACCTGTGCCGGGAtc  >  2:433372/1‑151 (MQ=255)                                                                                                                                         |                                                                                                                                                   GGATGGCGCGGGCTTTTCACTCTCGTGCGGCGTATCCGGCGAGAAATTTCCAGCCGGTTTGCGGGTCGAGAGCTGGCGTTCAATATCGGTCAGGTTTTCCAGCTTGCGGGTGGTGAGTTCCAGTTGCTGCTGTAGAACGTGATGTTGCTGGCGCAATGTATCCAGCTCGCTGTCGCTGGACTGCTGGAGTTTACTGTAACGTTGGCGCTCTTCCGCCAGTTGCAGTTGCAGCGCCTGACCATCGCGCCAGAGTTGATACAGTGGGCGAACCTGTGCCGGGATC  >  NC_000913/2688823‑2689105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑