Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	309,778	C→T	L83L (TTG→TTA)	ecpB ←	ECP production pilus chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	309,778	0	C	T	100.0%	112.2 / NA	30	L83L (TTG→TTA)	ecpB	ECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/14);  total (16/14)

GTGGCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCGGTACTGTTTTTGATCGTTTTGCTCAGC  >  NC_000913/309632‑309919                                                                                                                                                   |                                                                                                                                              gTGGCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGc                                                                                                                                           <  2:220933/151‑1 (MQ=255)   ggCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGTGCCTGATCAAACCAGACAATGCGGTAGTAGCTCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCTCTCGCTTGTGCGGGTAGCAGTAAGCt                                                                                                                                          <  1:130523/150‑1 (MQ=255)    gCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTgg                                                                                                                                        <  2:258648/151‑1 (MQ=255)       acaGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCgg                                                                                                                                     <  1:627039/151‑1 (MQ=255)         aGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGga                                                                                                                                   <  1:628219/151‑1 (MQ=255)                   ttGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGc                                                                                                                          >  2:95729/1‑150 (MQ=255)                     ggCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCaa                                                                                                                        >  1:368686/1‑150 (MQ=255)                      gCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACt                                                                                                                      <  2:252799/151‑1 (MQ=255)                            tCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCg                                                                                                                >  2:35932/1‑151 (MQ=255)                             cgcgCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCgg                                                                                                               >  1:652986/1‑151 (MQ=255)                                  tGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGt                                                                                                          >  2:567656/1‑151 (MQ=255)                                            tGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGGAGCAACTCATCCGGCTTGTCCaat                                                                                                     >  1:216203/1‑144 (MQ=255)                                              cgggCCTGATCAAATCAGACATTGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGCAGAAGCGGATCACTTCGATGGCTTGGGCGGGTAGCAGTAAGCCGGCGGGAGAGAGTAGCAACTCATCCGGCTTGTTCCTTGAGATa                                                                                               >  1:436455/2‑150 (MQ=255)                                                                 cAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCgg                                                                           <  1:287568/151‑1 (MQ=255)                                                                 cAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGAGAGTAACAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCgg                                                                           >  1:423276/1‑151 (MQ=255)                                                                    tGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTg                                                                         >  1:641874/1‑150 (MQ=255)                                                                              gcgcTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGt                                                              <  2:381115/151‑1 (MQ=255)                                                                                gcTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTc                                                            <  1:31606/151‑1 (MQ=255)                                                                                     ttttCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGAc                                                       >  1:160259/1‑151 (MQ=255)                                                                                      tttCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGAc                                                       <  1:569829/150‑1 (MQ=255)                                                                                            atGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGAGAATAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGACCGGCGGTAAGCGGTGAAGAGAGCCGTTCGAGACGGatat                                                 >  1:45345/2‑150 (MQ=255)                                                                                                     ccTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCg                                       >  2:42241/1‑151 (MQ=255)                                                                                                       ttATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGAc                                     >  2:457939/1‑151 (MQ=255)                                                                                                           agaagaAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCAc                                  <  2:139174/150‑1 (MQ=255)                                                                                                                aagCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCgg                            >  1:346303/1‑151 (MQ=255)                                                                                                                     gATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCGGTACTg                       <  1:514189/151‑1 (MQ=255)                                                                                                                     gATCACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCGGTACTg                       <  1:156613/151‑1 (MQ=255)                                                                                                                        cACTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCGGTACTGtt                     <  1:15973/150‑1 (MQ=255)                                                                                                                          cTTCGCTGGCTTGGGCGGGTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGGGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCGGTACTGttttt                  >  1:618280/1‑151 (MQ=255)                                                                                                                                          gggTAGCAGTAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCGGTACTGTTTTTGATCGTTTTGCTCAGc  >  2:474573/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                              GTGGCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGCGGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCGGTACTGTTTTTGATCGTTTTGCTCAGC  >  NC_000913/309632‑309919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑