Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,785,581	T→C	intergenic (‑570/+181)	ypjC ← / ← ileY	pseudogene/tRNA‑Ile

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,785,581	0	T	C	100.0%	141.1 / NA	37	intergenic (‑570/+181)	ypjC/ileY	pseudogene/tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/17);  total (20/17)

GCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAG  >  NC_000913/2785433‑2785717                                                                                                                                                     |                                                                                                                                         gCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCAc                                                                                                                                        <  2:356/151‑1 (MQ=255)    aaCAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACttt                                                                                                                                     >  2:550892/1‑151 (MQ=255)     aCAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACtttt                                                                                                                                    <  1:471786/151‑1 (MQ=255)     aCAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACtttt                                                                                                                                    <  2:146648/151‑1 (MQ=255)      cAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTg                                                                                                                                   <  2:104327/151‑1 (MQ=255)           aaTAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATg                                                                                                                               >  2:542142/1‑150 (MQ=255)               gTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTAc                                                                                                                          >  2:575602/1‑151 (MQ=255)                      gATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATgg                                                                                                                    >  2:109674/1‑150 (MQ=255)                         ttGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATa                                                                                                                 <  2:456611/150‑1 (MQ=255)                          tGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATaa                                                                                                                <  2:609859/150‑1 (MQ=255)                          tGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATaa                                                                                                                >  1:577058/1‑150 (MQ=255)                           gAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATaaa                                                                                                               <  2:238176/150‑1 (MQ=255)                                  tAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGc                                                                                                       >  1:142763/1‑151 (MQ=255)                                      cTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTg                                                                                                   >  2:382991/1‑151 (MQ=255)                                               tGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCAc                                                                                          >  2:583247/1‑151 (MQ=255)                                                 aaGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGt                                                                                        >  2:202671/1‑151 (MQ=255)                                                 aaGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGt                                                                                        <  1:555390/151‑1 (MQ=255)                                                    gggTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTAcc                                                                                     >  2:470184/1‑151 (MQ=255)                                                       tataATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCaa                                                                                   >  2:567888/1‑150 (MQ=255)                                                        ataataAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCaaa                                                                                  >  2:176097/1‑150 (MQ=255)                                                         taataaATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGCCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGCCGTGCCACTTACCaaaa                                                                                 >  1:559649/1‑150 (MQ=255)                                                           ataaATGATAATACTCTGGCATTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAaca                                                                               >  1:18858/1‑150 (MQ=255)                                                               aTGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGc                                                                          >  2:152337/1‑151 (MQ=255)                                                                tGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGcc                                                                         >  2:336793/1‑151 (MQ=255)                                                                 gATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGcc                                                                         <  2:383400/150‑1 (MQ=255)                                                                  ataataCTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCt                                                                       <  1:199772/151‑1 (MQ=255)                                                                       aCTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTc                                                                  >  1:192055/1‑151 (MQ=255)                                                                                ttATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAacca                                                           <  1:545571/149‑1 (MQ=255)                                                                                        aaTTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCAc                                                 <  2:623867/151‑1 (MQ=255)                                                                                               tAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCATGCATTTTTTCCAGTCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCTCActtctt                                            <  1:435286/149‑1 (MQ=255)                                                                                                 aTTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCtt                                        >  2:316467/1‑151 (MQ=255)                                                                                                 aTTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCtt                                        <  2:631624/151‑1 (MQ=255)                                                                                                 aTTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCtt                                        >  2:555275/1‑151 (MQ=255)                                                                                                                         gCTTGGCATAGCAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAatat                <  2:20228/151‑1 (MQ=255)                                                                                                                                    cAGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATc     >  2:646417/1‑151 (MQ=255)                                                                                                                                     aGGCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCg    <  2:565474/151‑1 (MQ=255)                                                                                                                                       gCATTTTTTCCAGGCACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAg  <  2:369250/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                         GCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGATAAATACATTGCAGTGGCGTGCCACGTACCAAAACACCAGCCCTCATTCGAAACCACCCACCGCACTTCTTCCTTGAAATGGCGTTAGTCATGAAATATAGACCGCCATCGAG  >  NC_000913/2785433‑2785717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑