| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,892,270 | A→G | P19P (CCA→CCG) | queD → | 6‑pyruvoyl tetrahydrobiopterin synthase (PTPS) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,892,270 | 0 | A | G | 91.4% | 112.6 / ‑4.4 | 35 | P19P (CCA→CCG) | queD | 6‑pyruvoyl tetrahydrobiopterin synthase (PTPS) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (17/15); minor base C (3/0); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
CAGCGATTTTGCGCAACAAAGTCGTTTTAGATAATGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGA > NC_000913/2892122‑2892413 | cAGCGATTTTGCGCAACAAAGTCGTTTTAGATAATGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACcgca < 1:605741/151‑1 (MQ=255) gATTTTGCGCAACAAAGTCGTTTTAGATAATGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTc > 2:382843/1‑151 (MQ=255) ttttGCGCAACAAAGTCGTTTTAGATAATGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGTTTTTTTGTCATGTAGAGAAAATATGATGTCCACCCAGTTATTTTAAAATTTTACCTTCTAAGGCGCCCACCGCTTACCGCC‑CGTccc > 1:343861/1‑151 (MQ=255) tttGCGCAACAAAGTCGTTTTAGATAATGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTccc < 2:306639/150‑1 (MQ=255) cGTTTTAGATAATGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGTTTTATCTTCCTGTAGAGAAAATATGATGTTCACCACGTTTTTTTAAGATTTTACCTTCGAAGCCGCCCACCCCTTACCCCA‑CGTCCCGGGAGGGCATaaa > 2:151189/1‑148 (MQ=255) cGTTTTAGATAATGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAAtg < 2:400541/150‑1 (MQ=255) tAGATAATGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGGTTTTTAGCCCTGTTAAGAAAATGGTATGGCCACCCAATTTTTTTAAAATTTTGCCTTTGAAGGCGCTGAACCCTTTCCCCACCGGCCCGGAgggggtaaaaatg > 1:664755/1‑138 (MQ=255) gATAATGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGcc < 2:559672/151‑1 (MQ=255) tGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGTTTTTTCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTTAAGATTTCCCCTTCGAAGCCGCCCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCAc > 2:548302/1‑151 (MQ=255) aaCAGCCTTTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTc < 1:416422/151‑1 (MQ=255) aCAGCCTTTCCGGTACTCTACGGCGGTTTTTTCGTCCTGTTGAGAAATTTTTATGTCCCCCACGTTATTTTAAAATTTTAACCTCCAAGCCGCTCCCCCCTTACCCCA‑CCTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTcc > 1:74999/1‑151 (MQ=255) aGCCTTTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGGAAATATGGTGTCCACCACGTTATTTTAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCCCGGGCATTCCtt > 2:104243/1‑151 (MQ=255) gcggTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTa < 1:826/151‑1 (MQ=255) aTCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGaa < 2:504245/151‑1 (MQ=255) tGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATc > 1:70893/1‑151 (MQ=255) gTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATcc > 2:506893/1‑151 (MQ=255) agagAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGc > 2:642016/1‑151 (MQ=255) aaTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACg < 1:379529/151‑1 (MQ=255) aTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACgg < 2:655425/151‑1 (MQ=255) atgatgTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCt > 1:441008/1‑151 (MQ=255) tgTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGAt > 1:594885/1‑151 (MQ=255) caccacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATc < 2:106966/151‑1 (MQ=255) cacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGAt > 2:65471/1‑151 (MQ=255) cacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGAt > 2:9984/1‑151 (MQ=255) tttAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTg > 2:61929/1‑151 (MQ=255) tttAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTg > 1:286884/1‑151 (MQ=255) aCCTTCGAAGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAgcgg > 2:369511/1‑151 (MQ=255) aaGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTaa < 1:423564/151‑1 (MQ=255) aGCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTaaa < 1:311574/151‑1 (MQ=255) gCCGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAaac < 2:125200/151‑1 (MQ=255) ccGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAaacc < 1:678651/151‑1 (MQ=255) cGCTCACCGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAaacca < 2:432428/151‑1 (MQ=255) cGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTTGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACg > 1:180135/1‑151 (MQ=255) cGCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGTTCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACg > 1:550041/1‑151 (MQ=255) gCTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGa < 1:451352/151‑1 (MQ=255) cTTACCGCA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACg > 1:340373/1‑149 (MQ=255) | CAGCGATTTTGCGCAACAAAGTCGTTTTAGATAATGCGAAAAAACAGCCTTTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACA‑CGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGA > NC_000913/2892122‑2892413 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |