Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,910,582	T→C	Y35C (TAC→TGC)	mazG ←	nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,910,582	0	T	C	100.0%	138.2 / NA	37	Y35C (TAC→TGC)	mazG	nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/18);  total (19/18)

ATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGG  >  NC_000913/2910437‑2910717                                                                                                                                                  |                                                                                                                                        atCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGTGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGgc                                                                                                                                    <  1:660082/151‑1 (MQ=255)   cATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGgcg                                                                                                                                   >  2:237813/1‑150 (MQ=255)       aaaGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAAtg                                                                                                                              >  1:367010/1‑151 (MQ=255)       aaaGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAAt                                                                                                                               <  1:243077/150‑1 (MQ=255)        aaGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAAtgg                                                                                                                             <  2:313777/151‑1 (MQ=255)        aaGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAAtgg                                                                                                                             >  1:278240/1‑151 (MQ=255)         aGTCAACGCGCCCTTCGTCCTGAGCCATTTGCGCGTAAAACACCGCCTGGAATAGCAGATCGCCCAGTTCCCCGTGTAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAAtgg                                                                                                                             <  1:136089/150‑1 (MQ=255)         aGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAAtggt                                                                                                                            >  2:652756/1‑151 (MQ=255)                  gcCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGt                                                                                                                   >  2:301907/1‑151 (MQ=255)                  gcCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGt                                                                                                                   >  1:300838/1‑151 (MQ=255)                         ttcCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGctct                                                                                                            >  1:370468/1‑151 (MQ=255)                         ttcCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGctct                                                                                                            >  1:517287/1‑151 (MQ=255)                                      tGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGc                                                                                               >  1:20326/1‑151 (MQ=255)                                       gcgcGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCa                                                                                              <  2:144361/151‑1 (MQ=255)                                       gcgcGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTAGCCCACGGGCa                                                                                              <  1:411553/151‑1 (MQ=255)                                        agcgTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGc                                                                                               >  1:227995/2‑149 (MQ=255)                                           gTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGcc                                                                                           <  1:149459/150‑1 (MQ=255)                                                       cTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATc                                                                               >  1:53419/1‑150 (MQ=255)                                                        tGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATcgc                                                                             >  1:125517/1‑151 (MQ=255)                                                          gAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCgcgc                                                                           >  1:309564/1‑151 (MQ=255)                                                              aGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGgc                                                                       <  1:117687/151‑1 (MQ=255)                                                                 aGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCt                                                                    >  1:400701/1‑151 (MQ=255)                                                                       cccAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCataa                                                              <  1:198231/151‑1 (MQ=255)                                                                                  gcgAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCaa                                                    >  1:367728/1‑150 (MQ=255)                                                                                      aGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGt                                               >  2:413329/1‑151 (MQ=255)                                                                                          cGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGAt                                           <  1:533167/151‑1 (MQ=255)                                                                                             cAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGAttt                                         <  2:220467/150‑1 (MQ=255)                                                                                                     tCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCAtt                                 <  2:437752/150‑1 (MQ=255)                                                                                                     tCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCAtt                                 <  2:411460/150‑1 (MQ=255)                                                                                                          ggCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTg                           >  1:349912/1‑151 (MQ=255)                                                                                                          ggCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTg                           <  1:215440/151‑1 (MQ=255)                                                                                                            cGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTc                         <  1:376174/151‑1 (MQ=255)                                                                                                             gATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTcc                        <  2:50715/151‑1 (MQ=255)                                                                                                                ggCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTg                      <  2:182707/150‑1 (MQ=255)                                                                                                                            ttCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGt          >  1:184895/1‑150 (MQ=255)                                                                                                                            ttCGTAGGTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTc         >  2:261628/1‑151 (MQ=255)                                                                                                                                   gTTTCTTCAAGGGTGCAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAgg  <  2:495443/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                        ATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCGCAATGGTGGCAAATGTCTGCTCTTTATCCCACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGG  >  NC_000913/2910437‑2910717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑