Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	318,798	T→C	M402V (ATG→GTG)	rclA ←	reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	318,798	0	T	C	100.0%	170.5 / NA	44	M402V (ATG→GTG)	rclA	reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/20);  total (24/20)

TGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATC  >  NC_000913/318653‑318942                                                                                                                                                  |                                                                                                                                                 tGTTCTGGCGTCTGATTTTGAATTTATTTGACTAATGAAAATAGATCATTGAGTGATTCTCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGACATCCATCACCATTTTCACTATATTGATCAcctc                                                                                                                                               <  1:369856/149‑1 (MQ=255)  gTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTg                                                                                                                                            <  1:158477/151‑1 (MQ=255)     cTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTggg                                                                                                                                          >  2:133665/1‑150 (MQ=255)       ggCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGt                                                                                                                                       <  1:646390/151‑1 (MQ=255)             tgtttttGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAacac                                                                                                                                 >  1:507391/4‑151 (MQ=255)              gATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAacaca                                                                                                                                <  1:481620/151‑1 (MQ=255)               aTTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAacacac                                                                                                                               >  2:462240/1‑151 (MQ=255)                    gAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACAcagc                                                                                                                            >  2:611838/1‑149 (MQ=255)                    gAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGGGGGAGTCAAcacacatc                                                                                                                            >  1:602186/1‑147 (MQ=255)                              gACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCt                                                                                                                <  2:429264/151‑1 (MQ=255)                                  aaTGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAAc                                                                                                             <  1:126578/150‑1 (MQ=255)                                  aaTGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAAc                                                                                                             >  1:321085/1‑150 (MQ=255)                                   aTGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACAt                                                                                                           <  2:663716/151‑1 (MQ=255)                                                          tCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGAACACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATcaaca                                                                                     >  2:238222/1‑150 (MQ=255)                                                                aTCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCtt                                                                              <  1:667024/151‑1 (MQ=255)                                                                  cGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCtttt                                                                            <  1:19293/151‑1 (MQ=255)                                                                  cGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCttt                                                                             <  1:470532/150‑1 (MQ=255)                                                                  cGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCttt                                                                             <  1:71787/150‑1 (MQ=255)                                                                             tAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACg                                                                 >  1:418522/1‑151 (MQ=255)                                                                             tAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACg                                                                 >  2:58458/1‑151 (MQ=255)                                                                                 tatCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGt                                                              >  1:317062/1‑150 (MQ=255)                                                                                  atCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTa                                                             <  2:634151/150‑1 (MQ=255)                                                                                  atCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTAt                                                            >  2:38885/1‑151 (MQ=255)                                                                                   tCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTAtc                                                           >  2:420189/1‑151 (MQ=255)                                                                                       aTCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTAtcat                                                         <  1:17355/149‑1 (MQ=255)                                                                                         cgcgTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTAtcattcat                                                     <  1:351812/151‑1 (MQ=255)                                                                                         cgcgTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTAtcattca                                                      <  1:62464/150‑1 (MQ=255)                                                                                             tAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCAct                                                 >  1:659550/1‑151 (MQ=255)                                                                                             tAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCAc                                                  <  1:365272/150‑1 (MQ=255)                                                                                               atatGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCActct                                               <  1:258140/151‑1 (MQ=255)                                                                                                      ataAGGCAGCCCGGCATCCATCCCCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAcg                                         <  1:413702/150‑1 (MQ=255)                                                                                                      ataAGGCAGCCCGGCATCCATCACTATTTTCACTATATTGATCACCTCGTGGGAGGCAACACACAGCAGTGTTGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAcgc                                        >  1:562096/1‑151 (MQ=255)                                                                                                        aaGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAcgcg                                       <  1:616688/150‑1 (MQ=255)                                                                                                               cccGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGc                                >  2:378334/1‑150 (MQ=255)                                                                                                                  ggCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCt                            >  2:340528/1‑151 (MQ=255)                                                                                                                  ggCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCt                            <  2:543236/151‑1 (MQ=255)                                                                                                                   gCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCt                            >  2:40707/1‑150 (MQ=255)                                                                                                                      tccatcACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTAc                          >  2:88141/1‑149 (MQ=255)                                                                                                                         atcACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGc                      >  2:560648/1‑150 (MQ=255)                                                                                                                         atcACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGCTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGc                      >  2:53805/1‑150 (MQ=255)                                                                                                                              cATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTc                >  1:545310/1‑151 (MQ=255)                                                                                                                               aTTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTc                >  2:329808/1‑150 (MQ=255)                                                                                                                                  ttCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTcacc             >  2:393827/1‑150 (MQ=255)                                                                                                                                      cTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCt         <  1:107072/150‑1 (MQ=255)                                                                                                                                            tGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATc  >  1:358754/1‑151 (MQ=255)                                                                                                                                              atcaccTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATc  >  2:312421/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                 TGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATC  >  NC_000913/318653‑318942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑