Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,978,279	A→G	A207A (GCT→GCC)	lysA ←	diaminopimelate decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,978,279	0	A	G	100.0%	153.8 / NA	41	A207A (GCT→GCC)	lysA	diaminopimelate decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/14);  total (27/14)

ATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCC  >  NC_000913/2978136‑2978425                                                                                                                                                |                                                                                                                                                   aTTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGcaccac                                                                                                                                             >  2:293918/1‑151 (MQ=255)    tGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACcacac                                                                                                                                           >  2:462112/1‑150 (MQ=255)    tGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACcacac                                                                                                                                           >  2:642118/1‑150 (MQ=255)    tGCTCACGCGCGGCACTCCACAGACCATAATAATGTTCGGTATCAACGGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACcacac                                                                                                                                           >  1:466392/1‑150 (MQ=255)          cgcgcgGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGtt                                                                                                                                    <  2:93532/151‑1 (MQ=255)                     ccACAGACAATAATAAGTTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCGCCACACGCCTGTTCCAGATggg                                                                                                                           <  1:437078/149‑1 (MQ=255)                          gACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATc                                                                                                                    >  2:537055/1‑151 (MQ=255)                              ataataATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAAc                                                                                                                 >  2:623644/1‑150 (MQ=255)                               taataaTGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGc                                                                                                               >  2:199011/1‑151 (MQ=255)                               taataaTGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGc                                                                                                               >  2:12857/1‑151 (MQ=255)                                   aatGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGa                                                                                                           <  2:183516/151‑1 (MQ=255)                                      gTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAAcc                                                                                                        >  2:645128/1‑151 (MQ=255)                                             aTCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGCAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGc                                                                                                 >  1:436698/1‑151 (MQ=255)                                                 aCCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCAtgt                                                                                             <  1:548546/151‑1 (MQ=255)                                                     ccTCTTCACCCTTTTGATACGGAACAGAAAGCCCCCCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGTACCATGGCCCCACTCACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATCTGAAt                                                                                         <  1:638782/151‑1 (MQ=255)                                                      ctctTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATg                                                                                        >  2:470861/1‑151 (MQ=255)                                                             cccTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGAcc                                                                                  <  1:582518/150‑1 (MQ=255)                                                             cccTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGAcc                                                                                  >  2:603389/1‑150 (MQ=255)                                                              ccTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGAcc                                                                                  >  1:522094/1‑149 (MQ=255)                                                                 gTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGc                                                                               <  2:400704/149‑1 (MQ=255)                                                                         ggAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAgatgat                                                                     >  2:201173/1‑151 (MQ=255)                                                                           aaCAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGatgatg                                                                    >  2:331334/1‑150 (MQ=255)                                                                            aCAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGAc                                                                  <  2:27416/151‑1 (MQ=255)                                                                              aGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGt                                                                >  1:484958/1‑151 (MQ=255)                                                                                      cACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCAc                                                        >  1:19289/1‑151 (MQ=255)                                                                                            ccgcAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTcc                                                    >  2:478712/1‑149 (MQ=255)                                                                                               cAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTgc                                               >  1:588424/1‑151 (MQ=255)                                                                                                 gAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTgcgg                                             >  1:304624/1‑151 (MQ=255)                                                                                                 gAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTgcgg                                             >  2:611866/1‑151 (MQ=255)                                                                                                  aaaTAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTgcggc                                            <  2:57116/151‑1 (MQ=255)                                                                                                           gTAAATCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGc                                        >  2:513435/1‑146 (MQ=255)                                                                                                             aaaTCCTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGc                                        >  2:36000/1‑144 (MQ=255)                                                                                                                 ccTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTa                             <  1:627353/151‑1 (MQ=255)                                                                                                                 ccTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTa                             <  2:170405/151‑1 (MQ=255)                                                                                                                  cTGACCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTAc                            >  2:515949/1‑151 (MQ=255)                                                                                                                     aCCGAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTAcc                           >  1:275486/1‑149 (MQ=255)                                                                                                                        gAATTCGATGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAGATa                      <  2:582518/151‑1 (MQ=255)                                                                                                                                tGACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAGATACCGtgctt              >  2:6236/1‑151 (MQ=255)                                                                                                                                 gACCTGACGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAGATACCGtgctt              <  2:415030/150‑1 (MQ=255)                                                                                                                                       aCGCACCATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGttt       <  1:257269/151‑1 (MQ=255)                                                                                                                                             cATGGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGcc  >  1:167103/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                                   ATTTGCTCACGCGCGGCATTCCACAGACCATAATAATGTTCGGTATCAACCGCCTCTTCACCCTGTTGATAAGGAACAGAAAGCCCACCGCCCGCAGAAATAGCCTGTAAATCCTGACCGAATTCGATGACCTGACGCACCATAGCACCACACACCTGTTCCAGATGGGCATAATCAACGCCAGAACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCACGTCCAGTGCGGCGGGCAGATCGGTGTACCAGATACCGTGCTTGCTGTTTTCGCC  >  NC_000913/2978136‑2978425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑