Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,012,795	T→C	N482S (AAC→AGC)	yqeB ←	XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,012,795	0	T	C	100.0%	181.9 / NA	48	N482S (AAC→AGC)	yqeB	XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/24);  total (24/24)

GCCCTGATGCATCAACATCATTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGCACGGATCACCCGTC  >  NC_000913/3012650‑3012939                                                                                                                                                  |                                                                                                                                                 gCCCTGATGCATCAACATCATTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTcaa                                                                                                                                              >  2:13148/1‑150 (MQ=255)    cTGATGCATCAACATCATTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCGGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTcaaca                                                                                                                                            >  1:641749/1‑149 (MQ=255)           aTCAACATCATTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGc                                                                                                                                    >  2:40413/1‑150 (MQ=255)               aCATCATTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCAt                                                                                                                               >  2:229549/1‑151 (MQ=255)               aCATCATTAACGCCTCAAGCACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCAt                                                                                                                               >  1:529320/1‑151 (MQ=255)                 atcatTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATcc                                                                                                                             >  2:501254/1‑151 (MQ=255)                   catTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATcccc                                                                                                                           <  1:686602/151‑1 (MQ=255)                   catTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATccc                                                                                                                            <  2:210799/150‑1 (MQ=255)                    atTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCg                                                                                                                          >  1:80501/1‑151 (MQ=255)                       aaCGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTc                                                                                                                        <  2:673266/150‑1 (MQ=255)                       aaCGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTc                                                                                                                        <  1:398682/150‑1 (MQ=255)                        aCGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCaa                                                                                                                      <  2:401123/151‑1 (MQ=255)                           ccTCAAGTACGCCGCCGCCAATCGCCCGGACTTTATCAGAAACGCGCGTGAAATCAGCGGTTTCACCAAGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGACGTCGCTCAACAAGCCACGCCCCATGCCCGTCAACg                                                                                                                    <  1:632962/150‑1 (MQ=255)                                  tACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCttt                                                                                                             >  2:342425/1‑150 (MQ=255)                                   aCGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTa                                                                                                            <  2:575668/150‑1 (MQ=255)                                             cAATCGCCCGGCCTTTGTCAGAAACGCTGGTGAAATCAGCGGTTTCACCCCGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCGCAACAACCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTc                                                                                                 >  1:173417/1‑151 (MQ=255)                                                 cGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCa                                                                                             <  2:500126/151‑1 (MQ=255)                                                 cGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCa                                                                                             <  1:209009/151‑1 (MQ=255)                                                   cccGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATACCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAAt                                                                                           >  2:207887/1‑151 (MQ=255)                                                     cGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATcc                                                                                         >  2:356447/1‑151 (MQ=255)                                                                aGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATcac                                                                               >  1:48319/1‑150 (MQ=255)                                                                 gAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATcaca                                                                              >  1:153707/1‑150 (MQ=255)                                                                  aaaCGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATc                                                                            <  1:599699/151‑1 (MQ=255)                                                                         ggTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCctc                                                                      <  1:601856/150‑1 (MQ=255)                                                                          gTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCtt                                                                    >  2:106539/1‑151 (MQ=255)                                                                            gAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCttt                                                                   <  1:67050/150‑1 (MQ=255)                                                                            gAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCttt                                                                   <  1:241634/150‑1 (MQ=255)                                                                               aTCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCAcc                                                               <  1:421861/151‑1 (MQ=255)                                                                                tCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCAcc                                                               >  2:342351/1‑150 (MQ=255)                                                                                    cGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCAcc                                                               >  2:285687/1‑146 (MQ=255)                                                                                             cacGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAttt                                                 <  2:186365/151‑1 (MQ=255)                                                                                              acGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTc                                                <  1:661902/151‑1 (MQ=255)                                                                                                gAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCACCTAATTTCAc                                              <  2:345431/151‑1 (MQ=255)                                                                                                 aGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACg                                             <  2:91427/151‑1 (MQ=255)                                                                                                       gATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGAt                                       <  1:361839/151‑1 (MQ=255)                                                                                                         tatCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCg                                     >  1:398979/1‑151 (MQ=255)                                                                                                          atCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGc                                    <  1:489934/151‑1 (MQ=255)                                                                                                             aCCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATa                                 <  2:653245/151‑1 (MQ=255)                                                                                                                  ttttAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGcc                            >  2:368185/1‑151 (MQ=255)                                                                                                                   tttAAAACCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCt                           >  2:236253/1‑151 (MQ=255)                                                                                                                      aaaaCCACCAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTgc                         >  2:83232/1‑150 (MQ=255)                                                                                                                         accaccAACCACTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTgcagca                     <  1:47225/151‑1 (MQ=255)                                                                                                                             ccaaccaCTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGa                  <  2:452252/150‑1 (MQ=255)                                                                                                                              caaccaCTGCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGc                >  1:344521/1‑151 (MQ=255)                                                                                                                                      gCCAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGCACGGATc         >  2:499995/1‑150 (MQ=255)                                                                                                                                       ccAGGCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGCACGGATCa        >  1:305859/1‑150 (MQ=255)                                                                                                                                          ggCCGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGCACGGATCACCCg    >  1:593598/1‑151 (MQ=255)                                                                                                                                            ccGTCGCTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGCACGGATCACCCGTc  <  1:491770/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 GCCCTGATGCATCAACATCATTAACGCCTCAAGTACGCCGCCGCCAATCGCCCGGGCTTTATCAGAAACGCTGGTGAAATCAGCGGTTTCACCACGAGGATCGATATCACCAATTTTAAAACCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGCAGGAGCACGGATCACCCGTC  >  NC_000913/3012650‑3012939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑