Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,122,268	T→C	D513G (GAC→GGC)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,122,268	0	T	C	100.0%	172.4 / NA	46	D513G (GAC→GGC)	glcB	malate synthase G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/26);  total (20/26)

GGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCA  >  NC_000913/3122122‑3122415                                                                                                                                                   |                                                                                                                                                    gggCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCg                                                                                                                                                  <  2:683669/150‑1 (MQ=255)           ggCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCcac                                                                                                                                       <  1:429826/151‑1 (MQ=255)             cTTGCACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGTAGCGTAGCAGCGGTTGGTGACGGCACCCAGGCTGGGTTTGCCCCGGCACGCGGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCCTCAGGCCCGGCATTGCCcaca                                                                                                                                      >  1:46331/1‑150 (MQ=255)              ttGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATg                                                                                                                                    >  1:367870/1‑151 (MQ=255)               tGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATg                                                                                                                                    <  1:322775/150‑1 (MQ=255)                  aCGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCt                                                                                                                                 <  2:660873/150‑1 (MQ=255)                     ctctGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTAc                                                                                                                             >  1:249304/1‑151 (MQ=255)                       ctGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTAcc                                                                                                                            >  2:330183/1‑150 (MQ=255)                             gTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTgc                                                                                                                     >  2:432291/1‑151 (MQ=255)                              ttggtttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTgc                                                                                                                     >  1:416152/1‑150 (MQ=255)                               tggtttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCACTGCCCACATGCCTTTACCAATTTgcgc                                                                                                                   >  1:671784/1‑151 (MQ=255)                                ggtttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCt                                                                                                                  <  2:331415/151‑1 (MQ=255)                                   ttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCtt                                                                                                                                <  1:317758/134‑1 (MQ=255)                                   ttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCtt                                                                                                                                >  2:317763/1‑134 (MQ=255)                                                 gcgcATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCcaca                                                                                                 <  2:686038/151‑1 (MQ=255)                                                       ggAGCGTAGCAGCGTTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTTCATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCacagaacag                                                                                           <  1:103506/151‑1 (MQ=255)                                                          gCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGAcc                                                                                        <  2:32978/151‑1 (MQ=255)                                                            gTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCgg                                                                                      <  2:371401/151‑1 (MQ=255)                                                               gcaggGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTGGGTCGCGCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCGTTGCCAATTGGCGCTTTACCGTGCAGCCCACAGAACAGTCCGGaaa                                                                                   <  1:547550/151‑1 (MQ=255)                                                               gcagcGGTTGGTGACGGAACCCAGGCTGTGGTTGCCCCCGCACGCAGTTGGTCGCCCTTCTGGCTGGACATGTCTGCCATCAGGCCCGGCATAGCCCACAGGCCTTAACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGaa                                                                                    <  1:299384/150‑1 (MQ=255)                                                                 agcGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGc                                                                                 >  1:527141/1‑151 (MQ=255)                                                                       tGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGtt                                                                            <  2:367883/150‑1 (MQ=255)                                                                        ggTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGttat                                                                          <  1:562627/151‑1 (MQ=255)                                                                                   ccAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAg                                                               <  2:409895/151‑1 (MQ=255)                                                                                       gCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCtt                                                           <  2:122337/151‑1 (MQ=255)                                                                                         tgtgTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCttt                                                          >  2:293855/1‑150 (MQ=255)                                                                                         tgtgTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTg                                                         >  2:470845/1‑151 (MQ=255)                                                                                          gtgtTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCttt                                                          <  2:18886/149‑1 (MQ=255)                                                                                          gtgtTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCttt                                                          <  2:258714/149‑1 (MQ=255)                                                                                           tgtTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGAt                                                       >  1:95101/1‑151 (MQ=255)                                                                                            gtTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGAt                                                       <  2:682628/150‑1 (MQ=255)                                                                                              ttGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCCCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATcc                                                     >  2:151383/1‑150 (MQ=255)                                                                                                  cccGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGc                                                <  1:25875/151‑1 (MQ=255)                                                                                                     ggCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTc                                             <  2:395840/151‑1 (MQ=255)                                                                                                            aGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTc                                       >  1:354095/1‑150 (MQ=255)                                                                                                                     cccTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGAtttt                             >  2:353075/1‑151 (MQ=255)                                                                                                                        ttCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCGTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTAc                          >  1:407929/1‑151 (MQ=255)                                                                                                                         tCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTa                           <  1:586668/149‑1 (MQ=255)                                                                                                                          cTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTa                           <  1:516080/148‑1 (MQ=255)                                                                                                                          cTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTa                           <  1:136711/148‑1 (MQ=255)                                                                                                                            ggCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACgc                        >  2:459322/1‑149 (MQ=255)                                                                                                                                  aCATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCgg                <  2:324607/151‑1 (MQ=255)                                                                                                                                   cATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCggg               >  2:61109/1‑151 (MQ=255)                                                                                                                                          gCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCtt        >  1:645248/1‑151 (MQ=255)                                                                                                                                                aGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATc   >  2:14659/1‑150 (MQ=255)                                                                                                                                                aGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCa  <  2:549187/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCA  >  NC_000913/3122122‑3122415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑