Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,355,080	T→C	V119A (GTC→GCC)	gltB →	glutamate synthase, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,355,080	0	T	C	100.0%	147.1 / NA	39	V119A (GTC→GCC)	gltB	glutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/22);  total (17/22)

CGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCT  >  NC_000913/3354933‑3355227                                                                                                                                                    |                                                                                                                                                    cgcAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGcccc                                                                                                                                                  >  2:637892/1‑151 (MQ=255)  gcAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCcccc                                                                                                                                                 >  1:136515/1‑151 (MQ=255)                ggCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGc                                                                                                                                     <  2:499526/149‑1 (MQ=255)                 gCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTg                                                                                                                                  <  2:268783/151‑1 (MQ=255)                               aCTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGc                                                                                                                    >  2:47588/1‑151 (MQ=255)                                  aCGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcct                                                                                                                 <  2:671587/151‑1 (MQ=255)                                      tGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctctcc                                                                                                             >  2:349916/1‑151 (MQ=255)                                      tGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctctcc                                                                                                             >  1:50002/1‑151 (MQ=255)                                         cGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctctcctct                                                                                                          >  2:321297/1‑151 (MQ=255)                                          gggATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCctctcctctc                                                                                                         <  2:128962/151‑1 (MQ=255)                                           ggATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCctctct                                                                                                        <  1:25439/151‑1 (MQ=255)                                               gCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGcc                                                                                                     >  1:185294/1‑150 (MQ=255)                                                     ccTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCAt                                                                                               <  2:553107/150‑1 (MQ=255)                                                        gAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTg                                                                                             <  2:251821/149‑1 (MQ=255)                                                        gAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGa                                                                                            <  2:188383/150‑1 (MQ=255)                                                          aTAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCa                                                                                         <  2:324597/151‑1 (MQ=255)                                                               gATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAAttt                                                                                    <  1:481412/151‑1 (MQ=255)                                                                      aaCTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGaa                                                                             <  1:47588/151‑1 (MQ=255)                                                                       aCTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAAc                                                                            <  2:30858/151‑1 (MQ=255)                                                                         tCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGc                                                                          >  2:488101/1‑151 (MQ=255)                                                                          cgccgcTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGc                                                                          <  1:118704/150‑1 (MQ=255)                                                                              gcTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCgg                                                                     >  2:316536/1‑151 (MQ=255)                                                                                tGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGc                                                                    >  2:414451/1‑150 (MQ=255)                                                                                  ccGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAgg                                                                 <  1:380581/151‑1 (MQ=255)                                                                                          cgcATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTc                                                         >  2:579509/1‑151 (MQ=255)                                                                                             aTCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCAc                                                      >  2:339734/1‑151 (MQ=255)                                                                                             aTCGCTGAAGAAGAACTGCAACGCGAAACCTTGCCGATTGTGGGCTGGCGTCATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCAc                                                      <  1:14006/151‑1 (MQ=255)                                                                                                  tGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGat                                                 >  1:400711/1‑151 (MQ=255)                                                                                                     agaagaaCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATg                                              <  1:509660/151‑1 (MQ=255)                                                                                                       aagaaCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATgg                                             >  2:313704/1‑150 (MQ=255)                                                                                                         gaaCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAgc                                          <  2:506902/151‑1 (MQ=255)                                                                                                         gaaCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAgc                                          >  1:260911/1‑151 (MQ=255)                                                                                                         gaaCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAgc                                          >  1:121211/1‑151 (MQ=255)                                                                                                               cAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGt                                     <  1:557000/150‑1 (MQ=255)                                                                                                                aaCGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCt                                   <  1:321510/151‑1 (MQ=255)                                                                                                                     gAAACCTTGTCGATTGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTa                              <  2:65728/151‑1 (MQ=255)                                                                                                                                  ttGTGGGCTGGCGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCccgccgccg                 >  2:128754/1‑151 (MQ=255)                                                                                                                                             cGTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGc      >  2:470673/1‑151 (MQ=255)                                                                                                                                              gTGATGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCg     <  2:505074/151‑1 (MQ=255)                                                                                                                                                 aTGCCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGtct  <  2:527928/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCT  >  NC_000913/3354933‑3355227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑