Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,370,354	A→G	V228A (GTG→GCG)	nanE ←	putative N‑acetylmannosamine‑6‑P epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,370,354	0	A	G	100.0%	144.1 / NA	40	V228A (GTG→GCG)	nanE	putative N‑acetylmannosamine‑6‑P epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/14);  total (26/14)

AATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACC  >  NC_000913/3370208‑3370497                                                                                                                                                   |                                                                                                                                                aaTGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGcgccgc                                                                                                                                             >  1:199436/1‑151 (MQ=255)  aTGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCgccgcc                                                                                                                                            >  1:153616/1‑151 (MQ=255)   tGCGGATAAGGCATCACGCAGGGCTTCGGGTGTCTGGCGGGCTGGCGTAGGAAGTTCCCGACGATCGGGGATCTCCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCgccgcc                                                                                                                                            <  2:292226/150‑1 (MQ=255)    gCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCtt                                                                                                                                          >  2:438197/1‑151 (MQ=255)      cgATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGAAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGACGATATCAATCGCCAGTGTGGTCATAGCGCCGCCtttt                                                                                                                                        >  2:54317/2‑151 (MQ=255)       gATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCttttt                                                                                                                                       >  1:330826/1‑151 (MQ=255)              cATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCt                                                                                                                                >  2:584126/1‑151 (MQ=255)                                   tCTGGTTGGCTGGAGTAGGAAGTCCCCGCCGATCGCGTATCTGCCCGGCAGCGCCAATCGGGGCGGGGGCGATTTTTTTGCCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAAt                                                                                                            <  1:514064/150‑1 (MQ=255)                                      ggcgggctgggGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAACCGCCGCGGCGGCAAGTTTTTTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACaa                                                                                                              <  1:369677/145‑1 (MQ=255)                                                    ggAAGTTCGCGGCGATCGCGGATCTGCCCATCAGCGCAAATCGGCGCGGGGGAAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGAcgcg                                                                                              <  2:270656/147‑1 (MQ=255)                                                         ttCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGc                                                                                       >  2:30488/1‑149 (MQ=255)                                                            acgacgATCGCGGATCTGCCCGGCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGaa                                                                                   <  1:627799/150‑1 (MQ=255)                                                              gacgaTCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAacc                                                                                 >  2:264377/1‑150 (MQ=255)                                                                 gaTCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAaccgacc                                                                             >  2:275509/1‑151 (MQ=255)                                                                     gcgGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTc                                                                          >  2:468595/1‑150 (MQ=255)                                                                     gcgGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTc                                                                          >  1:2078/1‑150 (MQ=255)                                                                       ggATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCAcc                                                                       >  1:68897/1‑151 (MQ=255)                                                                                 gTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACgcgc                                                             >  1:13744/1‑151 (MQ=255)                                                                                  tCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGcc                                                            >  1:540866/1‑151 (MQ=255)                                                                                    aGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGt                                                          >  2:21531/1‑151 (MQ=255)                                                                                          aaTCAGCGGGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgca                                                    <  2:578058/151‑1 (MQ=255)                                                                                           accAGCGGGGCGGAAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcat                                                   <  2:488928/149‑1 (MQ=255)                                                                                               gcgcGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcatcgc                                                >  1:611541/1‑150 (MQ=255)                                                                                                cgcgGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcatcgcat                                              >  1:325572/1‑151 (MQ=255)                                                                                                   ggcggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccg                                           >  2:356844/1‑151 (MQ=255)                                                                                                   ggcggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATcc                                            <  2:459177/150‑1 (MQ=255)                                                                                                      ggcAAGTTTATTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccgcc                                         <  2:188200/150‑1 (MQ=255)                                                                                                      ggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccgccg                                        >  2:201765/1‑151 (MQ=255)                                                                                                      ggaaaGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccgccg                                        <  2:509109/148‑1 (MQ=255)                                                                                                          attttAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCgccgcc                                      <  2:28260/147‑1 (MQ=255)                                                                                                           gTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTg                                    >  2:262875/1‑150 (MQ=255)                                                                                                           gTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTg                                    >  1:670947/1‑150 (MQ=255)                                                                                                            tttAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCt                                     >  2:597391/1‑148 (MQ=255)                                                                                                            tttAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTg                                    >  1:456322/1‑149 (MQ=255)                                                                                                                 tACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGc                             >  1:487908/1‑151 (MQ=255)                                                                                                                   ccgccgATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCgt                           <  2:218074/151‑1 (MQ=255)                                                                                                                    cgccgATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCgtg                          <  1:677003/151‑1 (MQ=255)                                                                                                                        gATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGtgttgt                      >  1:41464/1‑151 (MQ=255)                                                                                                                           atCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAAc                   >  1:233528/1‑151 (MQ=255)                                                                                                                                 cGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCtt             <  2:330832/151‑1 (MQ=255)                                                                                                                                  gCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGACCCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTc            <  2:684017/151‑1 (MQ=255)                                                                                                                                      gtgtGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGc         >  2:113044/1‑150 (MQ=255)                                                                                                                                            tCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCAcc  >  1:408236/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                AATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACC  >  NC_000913/3370208‑3370497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑