Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	379,831	A→G	R17R (CGT→CGC)	frmR ←	regulator protein that represses frmRAB operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	379,831	0	A	G	100.0%	174.5 / NA	46	R17R (CGT→CGC)	frmR	regulator protein that represses frmRAB operon
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/26);  total (20/26)

GTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATT  >  NC_000913/379684‑379977                                                                                                                                                    |                                                                                                                                                   gTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATgcg                                                                                                                                                  >  2:565705/1‑150 (MQ=255)    aaaCGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATgcgacg                                                                                                                                               >  1:275871/1‑150 (MQ=255)     aaCGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGcgacga                                                                                                                                              >  2:489331/1‑150 (MQ=255)          ttCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCg                                                                                                                                         >  1:23440/1‑150 (MQ=255)          ttCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCg                                                                                                                                         <  2:586380/150‑1 (MQ=255)                                   tCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTcc                                                                                                                <  1:385322/150‑1 (MQ=255)                                        cATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGCACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGt                                                                                                           >  1:307061/1‑150 (MQ=255)                                         aTCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTAc                                                                                                         >  2:638774/1‑151 (MQ=255)                                          tCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACt                                                                                                        >  1:482005/1‑151 (MQ=255)                                           cAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACt                                                                                                        <  2:604732/150‑1 (MQ=255)                                            aGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTgg                                                                                                      <  1:606876/151‑1 (MQ=255)                                             gCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTggg                                                                                                     <  2:158016/151‑1 (MQ=255)                                               ccATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGc                                                                                                    >  1:455729/1‑150 (MQ=255)                                                cATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCAt                                                                                                  <  1:279108/151‑1 (MQ=255)                                                cATTAGCCGCGCCCCGAACGCCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCAt                                                                                                  <  2:275697/151‑1 (MQ=255)                                                    aGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCg                                                                                              <  1:491842/151‑1 (MQ=255)                                                               gAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTcatcat                                                                                   <  2:135826/151‑1 (MQ=255)                                                                 aCGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCt                                                                                 <  2:679130/151‑1 (MQ=255)                                                                    gCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCtgc                                                                               >  1:566977/1‑150 (MQ=255)                                                                       gCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCtgcatgc                                                                           >  2:214396/1‑151 (MQ=255)                                                                                 ggAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACtat                                                                 <  2:215998/151‑1 (MQ=255)                                                                                 ggAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACta                                                                  <  1:85288/150‑1 (MQ=255)                                                                                           aCGGCATTCGGCATCACCGTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTAt                                                       >  2:126834/1‑151 (MQ=255)                                                                                           aCGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTAt                                                       <  1:207647/151‑1 (MQ=255)                                                                                           aCGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTAt                                                       >  1:584240/1‑151 (MQ=255)                                                                                            cGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTAtt                                                      >  1:368707/1‑151 (MQ=255)                                                                                              gCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCt                                                    >  1:646523/1‑151 (MQ=255)                                                                                                 ttCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCg                                                                                              <  1:37251/106‑1 (MQ=255)                                                                                                 ttCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCg                                                                                              >  2:37251/1‑106 (MQ=255)                                                                                                  tCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCtata                                                 <  2:586478/150‑1 (MQ=255)                                                                                                   cGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTatat                                                <  2:307732/150‑1 (MQ=255)                                                                                                       aTCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCaa                                           <  1:513047/151‑1 (MQ=255)                                                                                                         cACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAAt                                          <  1:448229/150‑1 (MQ=255)                                                                                                         cACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATg                                         <  1:590727/151‑1 (MQ=255)                                                                                                           cccTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCa                                       <  1:315342/151‑1 (MQ=255)                                                                                                               ccAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATAc                                    <  2:501649/150‑1 (MQ=255)                                                                                                                 aGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATAcccc                                 >  2:187976/1‑151 (MQ=255)                                                                                                                             agagCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGtatat                     >  1:73076/1‑151 (MQ=255)                                                                                                                                gCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATaa                   <  1:319215/150‑1 (MQ=255)                                                                                                                                gCATCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAac                  >  2:203384/1‑151 (MQ=255)                                                                                                                                  aTCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAacac                >  2:76633/1‑151 (MQ=255)                                                                                                                                  aTCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAacac                <  2:543331/151‑1 (MQ=255)                                                                                                                                  aTCAATCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAacac                <  2:372961/151‑1 (MQ=255)                                                                                                                                       tCTGCCCCCGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTgg             >  1:439227/1‑149 (MQ=255)                                                                                                                                           gccccGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGgag           <  1:636514/146‑1 (MQ=255)                                                                                                                                            ccccGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATa       >  2:363239/1‑150 (MQ=255)                                                                                                                                              ccGAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATaaaa    <  1:208434/151‑1 (MQ=255)                                                                                                                                                gAATGCGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAAtt  >  1:229283/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATT  >  NC_000913/379684‑379977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑