Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,634,685	G→A	intergenic (‑227/‑156)	yhiL ← / → yhiM	pseudogene, DUF4049 family protein/acid resistance protein, inner membrane

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,634,685	0	G	A	97.9%	177.1 / ‑4.1	47	intergenic (‑227/‑156)	yhiL/yhiM	pseudogene, DUF4049 family protein/acid resistance protein, inner membrane
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (25/21);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AATAATAATACTTATATGTTCGCTGATTTTAAGCACCGAGACTATCCGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGGCCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCATTAATGCATATTTCAATATTAGCA  >  NC_000913/3634538‑3634819                                                                                                                                                    |                                                                                                                                       aataataatACTTATATGTTCGCTGATTTTAAGCACCGAGACTATCCGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCt                                                                                                                                     >  2:90088/1‑151 (MQ=255)  ataataataCTTATATGTTCGCTGATTTTAAGCACCGAGACTATCCGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCtt                                                                                                                                    >  1:342336/1‑151 (MQ=255)      taataGTTATATGTTCGCTGATTTTAAGCACCGAGACTATCCGGGCTTTTACCAAAGAATGAAGAATAATCCATCCATGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTg                                                                                                                                 >  1:421567/1‑150 (MQ=255)      taataCTTATATGTTCGCTGATTTTAAGCACCGAGACTATCCGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGGCCTTTTg                                                                                                                                 >  2:445414/1‑150 (MQ=255)            ttATATGTTCGCTGATTTTAAGCACCGAGACTATCCGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTg                                                                                                                          >  2:292424/1‑151 (MQ=255)             tataTGTTCGCTGATTTTAAGCACCGAGACTATCCGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTg                                                                                                                          >  2:50024/1‑150 (MQ=255)                   ttCGCTGATTTTAAGCACCGAGACTATCCGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGt                                                                                                                    >  1:513642/1‑150 (MQ=255)                     cGCTGATTTTAAGCACCGAGACTATCCGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAgc                                                                                                                 >  2:193195/1‑151 (MQ=255)                           ttttAAGCACCGAGACTATCCGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTg                                                                                                           >  1:246711/1‑151 (MQ=255)                                            aTCCGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCta                                                                                          <  1:648027/151‑1 (MQ=255)                                            aTCCGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCta                                                                                          <  1:554843/151‑1 (MQ=255)                                              ccGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCtat                                                                                         >  2:290642/1‑150 (MQ=255)                                               cGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAg                                                                                       <  2:69008/151‑1 (MQ=255)                                                 gTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGtt                                                                                     <  1:84869/151‑1 (MQ=255)                                                     tttACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTtatgc                                                                                 >  1:72708/1‑151 (MQ=255)                                                     tttACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTtat                                                                                   <  1:109602/149‑1 (MQ=255)                                                      ttACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTtatgct                                                                                >  1:521483/1‑151 (MQ=255)                                                         ccAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTtatgctatg                                                                             >  2:190416/1‑151 (MQ=255)                                                            aataatGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCAt                                                                          <  2:492834/151‑1 (MQ=255)                                                                  gaagaaTAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATAATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCt                                                                    >  1:419009/1‑151 (MQ=255)                                                                   aagaaTAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGGTAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCtt                                                                   >  2:671326/1‑151 (MQ=255)                                                                            ccctccctGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGata                                                           <  1:63826/150‑1 (MQ=255)                                                                             cctccctGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATAtc                                                         <  2:170335/151‑1 (MQ=255)                                                                                      aaGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCAtt                                                 >  1:141172/1‑150 (MQ=255)                                                                                         aTAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTatat                                             <  1:386071/151‑1 (MQ=255)                                                                                         aTAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTatat                                             <  1:220225/151‑1 (MQ=255)                                                                                           aaTTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATAtt                                            >  1:7184/1‑150 (MQ=255)                                                                                                  aaaaaCAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGAt                                    <  1:190413/151‑1 (MQ=255)                                                                                                   aaaaCAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATg                                   >  1:422002/1‑151 (MQ=255)                                                                                                   aaaaCAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATg                                   >  2:430689/1‑151 (MQ=255)                                                                                                     aacaacAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGaa                                 <  1:150901/151‑1 (MQ=255)                                                                                                      acaacaGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGaaa                                <  1:118208/151‑1 (MQ=255)                                                                                                             cATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCAt                         >  2:96894/1‑151 (MQ=255)                                                                                                              aTAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATg                                   >  2:529049/1‑140 (MQ=255)                                                                                                              aTAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATg                                   <  1:529023/140‑1 (MQ=255)                                                                                                              aTAAGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCAtt                        <  2:515872/151‑1 (MQ=255)                                                                                                                aaGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCTTCATTATATTAACAGGATGAAATTATCATTa                       <  2:487509/150‑1 (MQ=255)                                                                                                                aaGAATTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCATTa                       <  2:270235/150‑1 (MQ=255)                                                                                                                   aaTTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCATTAATGc                   >  1:310424/1‑151 (MQ=255)                                                                                                                   aaTTAATCTTAGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCATTAATGc                   >  2:160571/1‑151 (MQ=255)                                                                                                                             aGGATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCATTAATGCATATTTCaa          <  2:811/150‑1 (MQ=255)                                                                                                                              ggATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCATTAATGCATATTTCAata        <  1:616259/151‑1 (MQ=255)                                                                                                                               gATAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCATTAATGCATATTTCAatat       >  2:416052/1‑151 (MQ=255)                                                                                                                                 tAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCATTAATGCATATTTCAATAtt      >  2:541761/1‑150 (MQ=255)                                                                                                                                 tAAATTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCATTAATGCATATTTCAATAtt      >  1:10150/1‑150 (MQ=255)                                                                                                                                    aTTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCATTAATGCATATTTCAATATTAGCa  <  2:419022/151‑1 (MQ=255)                                                                                                                                    aTTTTTATTTATCATGACCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCATTAATGCATATTTCAATATTAGCa  <  1:324125/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                       AATAATAATACTTATATGTTCGCTGATTTTAAGCACCGAGACTATCCGGTCTTTTACCAAATAATGAAGAATAATCCCTCCCTGGAAGATAATTGTAAAAAACAACAGCATAAGAATTAATCTTAGGATAAATTTTTATTTATCATGGCCTTTTGAACGCTGAAAAGTAGCGCTCTGCAAAGTAAACCCCGCTATAGTTATGCTATGCATTTGTCTTTAATGATATCTCGTCATTATATTAACAGGATGAAATTATCATTAATGCATATTTCAATATTAGCA  >  NC_000913/3634538‑3634819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑