Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,988,580	T→C	K163E (AAA→GAA)	hemX ←	putative uroporphyrinogen III methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,988,580	0	T	C	100.0%	170.4 / NA	44	K163E (AAA→GAA)	hemX	putative uroporphyrinogen III methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/24);  total (20/24)

AAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTTTGTTGGACTTCATCCAAC  >  NC_000913/3988439‑3988725                                                                                                                                              |                                                                                                                                                  aaaGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGc                                                                                                                                          >  2:314547/1‑151 (MQ=255)   aGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTg                                                                                                                                        <  2:5528/151‑1 (MQ=255)      tGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGc                                                                                                                                       >  2:196128/1‑149 (MQ=255)           aaGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTc                                                                                                                                <  1:390785/151‑1 (MQ=255)             gCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGt                                                                                                                              >  1:542819/1‑151 (MQ=255)             gCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGt                                                                                                                              <  2:464754/151‑1 (MQ=255)                     atgatgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCt                                                                                                                      <  1:518488/151‑1 (MQ=255)                     atgatgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCt                                                                                                                      <  1:48992/151‑1 (MQ=255)                       gatgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTg                                                                                                                     <  1:382986/150‑1 (MQ=255)                       gatgCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGa                                                                                                                    <  2:422121/151‑1 (MQ=255)                             gTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTc                                                                                                              >  2:637683/1‑151 (MQ=255)                             gTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTc                                                                                                              <  2:363717/151‑1 (MQ=255)                               cATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCca                                                                                                            <  2:476354/151‑1 (MQ=255)                                 tAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCcac                                                                                                           >  1:278817/1‑150 (MQ=255)                                    tCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCACCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCt                                                                                                       >  1:368218/1‑151 (MQ=255)                                       aCCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTtcc                                                                                                    <  2:119531/151‑1 (MQ=255)                                               tACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCCCCGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCgg                                                                                              <  1:189266/149‑1 (MQ=255)                                                    cAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGttt                                                                                       >  2:482340/1‑151 (MQ=255)                                                      gAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTc                                                                                      >  1:584538/1‑150 (MQ=255)                                                       aaaGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCAc                                                                                    >  2:338201/1‑151 (MQ=255)                                                        aaGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCAcc                                                                                   <  1:250423/151‑1 (MQ=255)                                                         aGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCAcc                                                                                   >  2:160909/1‑150 (MQ=255)                                                                cGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATc                                                                           <  2:659588/151‑1 (MQ=255)                                                                        tcGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTg                                                                     >  1:29731/1‑149 (MQ=255)                                                                               ttGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCcagc                                                              >  2:345641/1‑149 (MQ=255)                                                                                        gaACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGtttt                                                   >  1:321264/1‑151 (MQ=255)                                                                                             gTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGACAGCAGCCAGGTTTTAGCAt                                              <  2:122525/151‑1 (MQ=255)                                                                                               aaTCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTTCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATc                                             <  1:498212/150‑1 (MQ=255)                                                                                                  cAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCt                                          <  1:637648/150‑1 (MQ=255)                                                                                                  cAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCt                                          <  1:94258/150‑1 (MQ=255)                                                                                                   aGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGc                                        <  2:24812/151‑1 (MQ=255)                                                                                                   aGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGc                                        <  2:422687/151‑1 (MQ=255)                                                                                                             cattcatATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAAtgg                               <  1:502858/150‑1 (MQ=255)                                                                                                               ttcatATCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAAtggtgg                            >  1:562400/1‑151 (MQ=255)                                                                                                                    atCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGTCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGAcc                       <  2:607085/151‑1 (MQ=255)                                                                                                                    atCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGAcc                       >  1:241410/1‑151 (MQ=255)                                                                                                                     tCCGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGAcc                       <  1:373403/150‑1 (MQ=255)                                                                                                                       cGCCAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCttt                    >  1:599886/1‑151 (MQ=255)                                                                                                                          cAGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTttgt                 >  1:654136/1‑151 (MQ=255)                                                                                                                           aGGCTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTttgtt                >  1:453522/1‑151 (MQ=255)                                                                                                                              cTGGCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTTTGTTgg              >  2:456814/1‑150 (MQ=255)                                                                                                                                ggCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTTTGTTGGa             <  1:662755/149‑1 (MQ=255)                                                                                                                                ggCGTCTGCACTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTTTGTTGGACt           <  1:656411/151‑1 (MQ=255)                                                                                                                                         aCTTTCCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTTTGTTGGACTTCATCCAAc  >  1:310230/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                  AAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAGCATCGCTGCCGGAAATGGTGGCGACCTTTTGTTGGACTTCATCCAAC  >  NC_000913/3988439‑3988725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑