Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,011,598	T→C	W175R (TGG→CGG)	yigM →	putative inner membrane EamA‑like transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,011,598	0	T	C	100.0%	169.0 / NA	45	W175R (TGG→CGG)	yigM	putative inner membrane EamA‑like transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/26);  total (19/26)

GGGATTATTCGCTATGATCAGGTCACCGACCATTTCTGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGT  >  NC_000913/4011451‑4011739                                                                                                                                                    |                                                                                                                                              gggATTATTCGCTATGATCAGGTCACCGACCATTTCTGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGt                                                                                                                                            >  2:168371/1‑151 (MQ=255)          cGCTATGATCAGGTCACCGACCATTTCTGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCtt                                                                                                                                    >  1:207687/1‑150 (MQ=255)              aTGATCAGGTCACCGACCATTTCTGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGgcgc                                                                                                                               >  1:176496/1‑151 (MQ=255)              aTGATCAGGTCACCGACCATTTCTGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGgc                                                                                                                                 <  2:264656/149‑1 (MQ=255)                 aTCAGGTCACCGACCATTTCTGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGtt                                                                                                                            <  2:608141/151‑1 (MQ=255)                 aTCAGGTCACCGACCATTTCTGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGtt                                                                                                                            <  2:353451/151‑1 (MQ=255)                      gTCACCGACCATTTCTGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCtgg                                                                                                                       <  1:119971/151‑1 (MQ=255)                        cACCGACCATTTCTGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCtggtg                                                                                                                     <  2:158851/151‑1 (MQ=255)                                  ttCTGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGAtt                                                                                                            <  1:230929/150‑1 (MQ=255)                                   tCTGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTg                                                                                                           <  1:616577/150‑1 (MQ=255)                                     tGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCAt                                                                                                        >  2:272580/1‑151 (MQ=255)                                      ggACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATg                                                                                                       >  2:50993/1‑151 (MQ=255)                                      ggACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATg                                                                                                       <  1:226923/151‑1 (MQ=255)                                           ggCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAATCTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGtt                                                                                                    <  1:358607/149‑1 (MQ=255)                                             cTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTg                                                                                                >  2:71632/1‑151 (MQ=255)                                               tgctgcTGGTGCAACTCTCCAATCTCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGCTGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGCGGCAGTGATTGCATGGGTCTTGc                                                                                               >  1:7421/1‑150 (MQ=255)                                                gctgctGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTg                                                                                             <  1:422563/151‑1 (MQ=255)                                                   gctgGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTggg                                                                                           <  1:220608/150‑1 (MQ=255)                                                         gCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATgcg                                                                                    <  2:311412/151‑1 (MQ=255)                                                         gCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATgc                                                                                     <  1:534177/150‑1 (MQ=255)                                                                tcCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGaaa                                                                              >  2:44660/1‑150 (MQ=255)                                                                  cAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGtt                                                                                                    <  2:362016/126‑1 (MQ=255)                                                                  cAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGtt                                                                                                    >  1:362004/1‑126 (MQ=255)                                                                      atCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCg                                                                        <  2:458410/150‑1 (MQ=255)                                                                      atCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGc                                                                       >  2:60666/1‑151 (MQ=255)                                                                      atCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGc                                                                       >  1:147128/1‑151 (MQ=255)                                                                      atCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGc                                                                       >  2:372434/1‑151 (MQ=255)                                                                      atCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGc                                                                       >  2:417492/1‑151 (MQ=255)                                                                       tCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGc                                                                       >  2:384035/1‑150 (MQ=255)                                                                          cTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAAc                                                                   >  2:70362/1‑151 (MQ=255)                                                                             ttGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCa                                                                 <  1:380493/150‑1 (MQ=255)                                                                                 cATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTg                                                            <  1:107209/151‑1 (MQ=255)                                                                                   ttGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGc                                                           >  2:527523/1‑150 (MQ=255)                                                                                          ggtggGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATgggg                                                    <  2:466394/150‑1 (MQ=255)                                                                                          ggtggGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGc                                                   <  1:666042/151‑1 (MQ=255)                                                                                             gggTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCAtt                                                <  2:409841/151‑1 (MQ=255)                                                                                                       cGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTc                                      <  1:476439/151‑1 (MQ=255)                                                                                                                      cGCCCGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCtt                       <  1:50993/151‑1 (MQ=255)                                                                                                                         ccGATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCg                    >  2:135494/1‑151 (MQ=255)                                                                                                                           gATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCggg                  >  1:407899/1‑151 (MQ=255)                                                                                                                           gATGCCACAGCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCggg                  <  2:608468/151‑1 (MQ=255)                                                                                                                                    gCATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTAc         >  2:664943/1‑151 (MQ=255)                                                                                                                                     cATAACGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACt        <  2:121540/151‑1 (MQ=255)                                                                                                                                        aaCGCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACttt      <  2:213224/150‑1 (MQ=255)                                                                                                                                           gCCTTTGCGCGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTAtgt  <  2:206907/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              GGGATTATTCGCTATGATCAGGTCACCGACCATTTCTGGACTGGCTTGCTGCTGGTGCAACTCTCCAATATCACTTTTGCCATTGGCATGGTGGGTTACAAACGCCTGATGGAAACTCGCCCGATGCCACAGCATAACGCCTTTGCGTGGTTCTATCTTGGCGCGTTTCTGGTGGCAGTGATTGCATGGTTCTTGCTGGGAAATGCGCAGAAAATGCCGCAAACCACGCTGCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGT  >  NC_000913/4011451‑4011739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑